Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst
The use of intramuscular injections of procaine penicillin G (bensylpenicillin procaine) in the horse is now and then associated with acute adverse reactions (penicillin-chock). The etiology is not yet clearly understood, but the theories are several. One possibility is that it can be caused by proc...
| Autor principal: | |
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| Formato: | Otro |
| Lenguaje: | sueco sueco |
| Publicado: |
2005
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| Materias: | |
| Acceso en línea: | https://stud.epsilon.slu.se/11527/ |
| _version_ | 1855571889628381184 |
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| author | Gustafsson, Linda |
| author_browse | Gustafsson, Linda |
| author_facet | Gustafsson, Linda |
| author_sort | Gustafsson, Linda |
| collection | Epsilon Archive for Student Projects |
| description | The use of intramuscular injections of procaine penicillin G (bensylpenicillin procaine) in the horse is now and then associated with acute adverse reactions (penicillin-chock). The etiology is not yet clearly understood, but the theories are several. One possibility is that it can be caused by procaine toxicity. Procaine penicillin G is a salt which is quickly dissolved in plasma. Procaine is then metabolized by plasmaesterases to non-toxic metabolites, p-aminobenzoic acid (PABA) and diethylaminoethanol. The purpose of this study was to investigate if there are any differences in the esterase activity in plasma between horses that had suffered from adverse reactions and a group of horses that had no reactions, following intramuscular injection of procaine penicillin G. Furthermore the rate of hydrolysis of procaine was investigated in a normal, healthy group of horses. In this group the possible variation of the procaine hydrolysis during a period of twenty-four hours was also examined. Procaine was incubated in horse plasma and the amount of PABA formed was measured by HPLC analysis. The results showed a tendency that the horses suffering from adverse reactions had a lower activity of procaine hydrolysis compared to the control group. The difference was not statistically significant at a level of 95% (the p-value was 0,076). The study also showed that there is a great individual variation in esterase activity in a normal population of horses. Furthermore there is no marked variation in esterase activity during a period of twenty-four hours. |
| format | Otro |
| id | RepoSLU11527 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish swe |
| publishDate | 2005 |
| publishDateSort | 2005 |
| record_format | eprints |
| spelling | RepoSLU115272017-10-03T10:49:09Z https://stud.epsilon.slu.se/11527/ Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst Gustafsson, Linda Veterinary science and hygiene - General aspects Faculty of Veterinary Medicine and Animal Science The use of intramuscular injections of procaine penicillin G (bensylpenicillin procaine) in the horse is now and then associated with acute adverse reactions (penicillin-chock). The etiology is not yet clearly understood, but the theories are several. One possibility is that it can be caused by procaine toxicity. Procaine penicillin G is a salt which is quickly dissolved in plasma. Procaine is then metabolized by plasmaesterases to non-toxic metabolites, p-aminobenzoic acid (PABA) and diethylaminoethanol. The purpose of this study was to investigate if there are any differences in the esterase activity in plasma between horses that had suffered from adverse reactions and a group of horses that had no reactions, following intramuscular injection of procaine penicillin G. Furthermore the rate of hydrolysis of procaine was investigated in a normal, healthy group of horses. In this group the possible variation of the procaine hydrolysis during a period of twenty-four hours was also examined. Procaine was incubated in horse plasma and the amount of PABA formed was measured by HPLC analysis. The results showed a tendency that the horses suffering from adverse reactions had a lower activity of procaine hydrolysis compared to the control group. The difference was not statistically significant at a level of 95% (the p-value was 0,076). The study also showed that there is a great individual variation in esterase activity in a normal population of horses. Furthermore there is no marked variation in esterase activity during a period of twenty-four hours. I samband med behandling av hästar med intramuskulära injektioner av bensylpenicillinprokain förekommer det ibland att hästen drabbas av en så kallad penicillinchock, vilken kan ha flera olika bakomliggande orsaker. Den vanligaste orsaken anses vara prokaintoxicitet. Bensylpenicillinprokain är ett salt som består av penicillin och prokain. Saltet löses snabbt upp när det når cirkulationen. I blodet hydrolyseras prokain av plasmaesteraser till icke-toxiska metaboliter, paraaminobenzoesyra (PABA) och dietylaminoetanol. Syftet med studien var att undersöka om hydrolyshastigheten av prokain i plasma skiljer sig mellan hästar som fått biverkningar och en kontrollgrupp som ej har fått biverkningar efter behandling med bensylpenicillinprokain. Dessutom undersöktes hastigheten med vilken prokain hydrolyseras hos en normalpopulation av hästar samt om nedbrytningsförmågan varierar under dygnet. För att bestämma hydrolyshastigheten av prokain inkuberades prokain i hästplasma och därefter mättes mängden bildad PABA genom analys med HPLC. Resultatet visar att det finns en tendens till att chockhästarna har en lägre esterasaktivitet jämfört med hästarna i kontrollgruppen, skillnaden är dock inte statistiskt signifikant på en 95 %-nivå (pvärdet var 0,076). Studien visar också att det föreligger en relativt stor variation i förmågan att hydrolysera prokain hos en normalpopulation hästar, samt att aktiviteten hos esteraserna inte verkar variera under dygnet. 2005-01-21 Other NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/11527/1/gustafsson_l_171003.pdf Gustafsson, Linda, 2005. Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst. UNSPECIFIED, Uppsala. Uppsala: (VH) > Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-713.html> urn:nbn:se:slu:epsilon-s-7441 swe |
| spellingShingle | Veterinary science and hygiene - General aspects Faculty of Veterinary Medicine and Animal Science Gustafsson, Linda Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst |
| title | Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst |
| title_full | Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst |
| title_fullStr | Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst |
| title_full_unstemmed | Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst |
| title_short | Betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst |
| title_sort | betydelsen av prokainets nedbrytning i plasma vid penicillinchock hos häst |
| topic | Veterinary science and hygiene - General aspects Faculty of Veterinary Medicine and Animal Science |
| url | https://stud.epsilon.slu.se/11527/ https://stud.epsilon.slu.se/11527/ |