Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP
Feline infectious peritonitis (FIP) is a feline viral disease with high mortality. There is no cure or any effective vaccine available today. Many questions are yet to be answered about this disease and the immune response in affected cats. The aim of the study is to evaluate two different techn...
| Autor principal: | |
|---|---|
| Formato: | L3 |
| Lenguaje: | sueco Inglés |
| Publicado: |
SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2009
|
| Materias: |
| _version_ | 1855571884880429056 |
|---|---|
| author | Karlsson, Marie |
| author_browse | Karlsson, Marie |
| author_facet | Karlsson, Marie |
| author_sort | Karlsson, Marie |
| collection | Epsilon Archive for Student Projects |
| description | Feline infectious peritonitis (FIP) is a feline viral disease with high mortality. There is no cure or any effective vaccine available today. Many questions are yet to be answered about this disease and the immune response in affected cats.
The aim of the study is to evaluate two different techniques for the study of cytokine profiles in cats vaccinated with a vaccine concept against FIPV. More information about the immune response in these cats could give valuable information to better understand the pathogenesis of the disease and the development of an effective vaccine.
With quantitative real-time PCR and antigen ELISA has the levels of the cytokines IFN-γ and IL-10 been studied in blood from SPF cats immunised with an ISCOM vaccine against FIPV and then challenged.
The levels of IFN-γ and IL-10 were too low in the serum samples to be able to analyze with the ELISA used. With real-time PCR, which is a very sensitive method, all the cats in the trial expressed IFN-γ and IL-10 at one or more samplings. No apparent difference was seen between the vaccinated and control cats.
Commercial ELISA can have a too high lowest detection level than what's useful when analyzing cytokines in samples from cats that have not developed FIP. For further analyzes of cytokines in very low concentrations a more sensitive method is needed, where proximity ligation assay is one possible alternative. |
| format | L3 |
| id | RepoSLU11501 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2009 |
| publishDateSort | 2009 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU115012017-10-04T07:43:35Z Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP Karlsson, Marie FIP cytokiner vaccination PCR ELISA Feline infectious peritonitis (FIP) is a feline viral disease with high mortality. There is no cure or any effective vaccine available today. Many questions are yet to be answered about this disease and the immune response in affected cats. The aim of the study is to evaluate two different techniques for the study of cytokine profiles in cats vaccinated with a vaccine concept against FIPV. More information about the immune response in these cats could give valuable information to better understand the pathogenesis of the disease and the development of an effective vaccine. With quantitative real-time PCR and antigen ELISA has the levels of the cytokines IFN-γ and IL-10 been studied in blood from SPF cats immunised with an ISCOM vaccine against FIPV and then challenged. The levels of IFN-γ and IL-10 were too low in the serum samples to be able to analyze with the ELISA used. With real-time PCR, which is a very sensitive method, all the cats in the trial expressed IFN-γ and IL-10 at one or more samplings. No apparent difference was seen between the vaccinated and control cats. Commercial ELISA can have a too high lowest detection level than what's useful when analyzing cytokines in samples from cats that have not developed FIP. For further analyzes of cytokines in very low concentrations a more sensitive method is needed, where proximity ligation assay is one possible alternative. Felin infektiös peritonit (FIP) är en virussjukdom hos katt med hög dödlighet som det varken finns behandling eller effektivt vaccin mot. Många frågor är obesvarade när det gäller den här sjukdomen och immunsvaret hos drabbade katter. Syftet med arbetet är att utvärdera två olika tekniker för att studera cytokinuttryck hos katter vaccinerade med vaccinkoncept mot FIPV. Mer information om immunsvaret hos dessa katter skulle kunna vara till hjälp vid förståelsen för sjukdomens patogenes och framställningen av ett effektivt vaccin. Med hjälp av kvantitativ realtids-PCR och antigen ELISA har nivåerna av cytokinerna IFN-� och IL-10 i blod studerats hos SPF-katter immuniserade med ett ISCOM-vaccin mot FIPV och därefter experimentellt infekterade. Nivåerna av IFN-� och IL-10 i serum var för låga att kunna analyserad med den testade ELISA:n. Med realtids-PCR, som är en mycket känslig metod, sågs genuttryck för IFN-� och IL-10 hos alla katterna i försöket vid ett eller flera provtagningstillfällen. Inga påtagliga skillnader sågs mellan vaccinerade katter jämfört med kontroller. Kommersiella ELISA kan ha för hög lägsta detektionsnivå än vad som är användbart vid analys av cytokiner hos katter som inte utvecklat FIP. För vidare analys av cytokiner i mycket låga koncentrationer i serum krävs en känsligare metod där proximitetsligerings assay är ett tänkbart alternativ. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2009 L3 swe eng https://stud.epsilon.slu.se/11501/ |
| spellingShingle | FIP cytokiner vaccination PCR ELISA Karlsson, Marie Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP |
| title | Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP |
| title_full | Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP |
| title_fullStr | Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP |
| title_full_unstemmed | Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP |
| title_short | Cytokinuttryck vid vaccination med olika vaccinkoncept mot FIP |
| title_sort | cytokinuttryck vid vaccination med olika vaccinkoncept mot fip |
| topic | FIP cytokiner vaccination PCR ELISA |