Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains
The aim of this project was to get clearer knowledge about the spread of Borna disease virus (BDV) by comparing sequences from BDV infected cats from different regions in Sweden. BDV is associated with staggering disease (SD), a feline neurological disorder that causes non-suppurative meningoencepha...
| Autor principal: | |
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| Formato: | L3 |
| Lenguaje: | Inglés sueco |
| Publicado: |
SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2008
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| Materias: |
| _version_ | 1855571879180369920 |
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| author | Schenck, Jessica |
| author_browse | Schenck, Jessica |
| author_facet | Schenck, Jessica |
| author_sort | Schenck, Jessica |
| collection | Epsilon Archive for Student Projects |
| description | The aim of this project was to get clearer knowledge about the spread of Borna disease virus (BDV) by comparing sequences from BDV infected cats from different regions in Sweden. BDV is associated with staggering disease (SD), a feline neurological disorder that causes non-suppurative meningoencephalomyelitis. However, cats showing signs for SD are not always confirmed to be BDV infected. In order to get a broader picture of the possible etiological cause/s of SD, molecular screening of viruses using random amplification and sequencing was performed. This study includes eight cats, whereas four were previously positive for BDV in nested RT-PCR and real-time RT-PCR analysis and four cats, only histopathology examined, where three showed signs for SD. Molecular epidemiology approach with RT-PCR and nested RT-PCR for previously positive cats gave PCR products at incorrect as well as correct sizes. However, sequencing showed only feline origin. Since real-time RT-PCR also was negative, one hypothesis is that the RNA had degraded or disappeared through the RNA extraction process. Nested PCR was also performed for the four new cats. Also here the sequencing showed only presence of feline origin. However, for these cats, real-time RT-PCR was positive for the pooled PCR product. Molecular screening of viruses for these four cats showed as before only sequences of feline origin. This project has identified some of the problems of studying RNA viruses present in small amounts in brain tissue. There is always a risk that the RNA is lost during the RNA extraction process. Therefore, further studies are needed to provide more information about the BDV molecular epidemiology in Sweden. |
| format | L3 |
| id | RepoSLU11469 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2008 |
| publishDateSort | 2008 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU114692017-10-04T11:44:41Z Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains Vingelsjuka hos katt i Sverige : utveckling av molekylära metoder för detektion och genetisk karaktärisering av Bornavirusstammar Schenck, Jessica BDV borna disease virus SD staggering disease RNA virus virus neurological disorder The aim of this project was to get clearer knowledge about the spread of Borna disease virus (BDV) by comparing sequences from BDV infected cats from different regions in Sweden. BDV is associated with staggering disease (SD), a feline neurological disorder that causes non-suppurative meningoencephalomyelitis. However, cats showing signs for SD are not always confirmed to be BDV infected. In order to get a broader picture of the possible etiological cause/s of SD, molecular screening of viruses using random amplification and sequencing was performed. This study includes eight cats, whereas four were previously positive for BDV in nested RT-PCR and real-time RT-PCR analysis and four cats, only histopathology examined, where three showed signs for SD. Molecular epidemiology approach with RT-PCR and nested RT-PCR for previously positive cats gave PCR products at incorrect as well as correct sizes. However, sequencing showed only feline origin. Since real-time RT-PCR also was negative, one hypothesis is that the RNA had degraded or disappeared through the RNA extraction process. Nested PCR was also performed for the four new cats. Also here the sequencing showed only presence of feline origin. However, for these cats, real-time RT-PCR was positive for the pooled PCR product. Molecular screening of viruses for these four cats showed as before only sequences of feline origin. This project has identified some of the problems of studying RNA viruses present in small amounts in brain tissue. There is always a risk that the RNA is lost during the RNA extraction process. Therefore, further studies are needed to provide more information about the BDV molecular epidemiology in Sweden. Syftet med projektet var att få mer kunskap om spridning av bornavirus (BDV) genom att jämföra sekvenser från BDV-infekterade katter från olika regioner i Sverige. BDV är associerat med vingelsjuka hos katt, en neurologisk sjukdom som karakteriseras av en non-purulent meningoencefalomyelit. Dock kan vingelsjuka inte alltid kopplas till BDV-infektion. För att få en bredare bild av tänkbara etiologiska orsaker till vingelsjuka utfördes även molekylär screening av virus med slumpmässig amplifiering och sekvensering. Här studeras åtta kattprover, varav fyra tidigare positiva för BDV vid PCR och realtids-PCR analyser. De resterande fyra katterna hade enbart undersökts histopatologiskt där tre visade tecken på vingelsjuka. PCRanalysen för tidigare positiva katter gav band vid fel eller korrekta storlekar. Sekvensering visade dock enbart PCR-produkt från katt. Eftersom även resultatet från realtids-PCR var negativt finns det risk för att RNA har degraderat eller försvunnit under extraheringen. PCR-analys utfördes för de fyra nya katterna som enbart studerats histopatologiskt. Även här visade sekvensering enbart RNA från katt. För dessa katter gav emellertid realtids-PCR ett positivt resultat för poolad PCR-produkt. Molekylär screening av virus för dessa fyra katter visade bara på värdprodukt för både DNA och RNA. Detta projekt visar problematiken med RNA-virus, som finns i små halter i hjärnvävnad. Det finns även en risk att RNA försvinner vid extrahering av RNA. Därför krävs ytterligare studier för att ge mer information om molekylär epidemiologi av BDV i Sverige. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2008 L3 eng swe https://stud.epsilon.slu.se/11469/ |
| spellingShingle | BDV borna disease virus SD staggering disease RNA virus virus neurological disorder Schenck, Jessica Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains |
| title | Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains |
| title_full | Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains |
| title_fullStr | Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains |
| title_full_unstemmed | Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains |
| title_short | Staggering disease in cats in Sweden : development of molecular methods for detection and genetic characterization of Borna disease virus strains |
| title_sort | staggering disease in cats in sweden : development of molecular methods for detection and genetic characterization of borna disease virus strains |
| topic | BDV borna disease virus SD staggering disease RNA virus virus neurological disorder |