Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud
Objective: To determine the frequency of antimicrobial resistance in bacteria from healthy Swedish dogs and whether they are carriers of methicillinresistant Staphylococcus aureus (MRSA). Material: Samples from skin from the perineal region and from faeces collected in the anus with cottonswab (...
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| Formato: | L3 |
| Lenguaje: | sueco Inglés |
| Publicado: |
SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231)
2007
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| Materias: |
| _version_ | 1855571850581508096 |
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| author | Zwenson, Johanna |
| author_browse | Zwenson, Johanna |
| author_facet | Zwenson, Johanna |
| author_sort | Zwenson, Johanna |
| collection | Epsilon Archive for Student Projects |
| description | Objective: To determine the frequency of antimicrobial resistance in bacteria from healthy Swedish dogs and whether they are carriers of methicillinresistant Staphylococcus aureus (MRSA).
Material: Samples from skin from the perineal region and from faeces collected in the anus with cottonswab (Copan) from 299 healthy Swedish dogs. Samples were collected at dog exhibitions.
Method: Specimens were submitted for bacterial culture where bacteria (Escherichia coli, enterococci and staphylococci) were isolated and identified by current methods. Antimicrobial susceptibility testing was performed with a microdilution method where MIC-values were determined. Staphylococci with high degree of resistance were tested for presence of mecA-gene by PCR.
Results: Looking at the whole material there were no difference in antimicrobial resistance compared with food-producing animals. No difference in resistance regarding age could be detected. Ampicillin and sulfametoxazole showed a higher degree of resistance in E. coli-isolates from dogs that had been treated with antibiotics during the last 12 months compared to non-treated dogs. One isolate of E. coli showed resistance against the third generation cephalosporines. A difference in species distribution was seen for enterococci between dogs, pigs and chickens. One methicillinresistant Staphylococcus intermedius (MRSI) was isolated which is the first isolate of this kind from a dog in Sweden. Compared with clinical S. intermedius-isolates from dogs there was a tendency of overall less resistance in the dogs in this study. On the other hand there was a tendency of a higher resistance compared to a small Swedish study performed ten years ago. |
| format | L3 |
| id | RepoSLU11322 |
| institution | Swedish University of Agricultural Sciences |
| language | swe Inglés |
| publishDate | 2007 |
| publishDateSort | 2007 |
| publisher | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| publisherStr | SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) |
| record_format | eprints |
| spelling | RepoSLU113222017-10-10T07:55:14Z Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud Antimicrobial resistance in bacteria from healthy dogs in Sweden : indicator bacteria – E coli & Enterococcus spp and Staphylococcus spp isolated from the skin Zwenson, Johanna cephalosporin antimikrobiell resistens hund frisk hund antibiotika MRSA MRSI E. coli Objective: To determine the frequency of antimicrobial resistance in bacteria from healthy Swedish dogs and whether they are carriers of methicillinresistant Staphylococcus aureus (MRSA). Material: Samples from skin from the perineal region and from faeces collected in the anus with cottonswab (Copan) from 299 healthy Swedish dogs. Samples were collected at dog exhibitions. Method: Specimens were submitted for bacterial culture where bacteria (Escherichia coli, enterococci and staphylococci) were isolated and identified by current methods. Antimicrobial susceptibility testing was performed with a microdilution method where MIC-values were determined. Staphylococci with high degree of resistance were tested for presence of mecA-gene by PCR. Results: Looking at the whole material there were no difference in antimicrobial resistance compared with food-producing animals. No difference in resistance regarding age could be detected. Ampicillin and sulfametoxazole showed a higher degree of resistance in E. coli-isolates from dogs that had been treated with antibiotics during the last 12 months compared to non-treated dogs. One isolate of E. coli showed resistance against the third generation cephalosporines. A difference in species distribution was seen for enterococci between dogs, pigs and chickens. One methicillinresistant Staphylococcus intermedius (MRSI) was isolated which is the first isolate of this kind from a dog in Sweden. Compared with clinical S. intermedius-isolates from dogs there was a tendency of overall less resistance in the dogs in this study. On the other hand there was a tendency of a higher resistance compared to a small Swedish study performed ten years ago. Syfte: Syftet med denna studie är att kartlägga förekomsten av resistens hos bakterier hos friska svenska hundar och att undersöka om det finns friska hundar som är bärare av MRSA (meticillinresistent Staphylococcus aureus). Material: Hudprov från perineum samt träckprov tagna med bomullspinne (Copanrör) från 299 friska svenska hundar deltagande i olika hundutställningar. Metod: Proverna odlades och bakterier (Escherichia coli, enterokocker och stafylokocker) isolerades och typades enligt gängse metoder och resistensbestämdes med mikrodilutionsmetod varvid MIC-värden erhölls. Stafylokocker som hade oxacillin- eller utvidgad resistens testades avseende mecA-genen med PCR. Resultat: För hela materialet sågs ingen skillnad i resistens hos indikatorbakterier hos hund jämfört med lantbrukets djur. Ingen åldersskillnad avseende resistens kunde påvisas. För ampicillin och sulfametoxazole sågs en signifikant ökning av antibiotikaresistensen hos E. coli isolerade från de hundar som behandlats med antibiotika senaste året. En E. coli med resistens mot tredje generationens cefalosporiner påvisades. En djurslagsskillnad, mellan hund, kyckling och svin, sågs i fördelningen av vilka species av enterokocker som förekom. En meticillinresistent Staphylococcus intermedius (MRSI), den första isolerad från hund i Sverige, påvisades. En tendens till lägre resistens sågs hos S. intermedius isolerade från hundarna i studien jämfört med kliniska prover från hundar (material från SVARM), däremot sågs en tendens av högre frekvens resistensfrekvens jämfört med en tidigare svensk studie baserad på ett mindre material, som utförts för tio år sedan. SLU/Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) 2007 L3 swe eng https://stud.epsilon.slu.se/11322/ |
| spellingShingle | cephalosporin antimikrobiell resistens hund frisk hund antibiotika MRSA MRSI E. coli Zwenson, Johanna Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud |
| title | Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud |
| title_full | Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud |
| title_fullStr | Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud |
| title_full_unstemmed | Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud |
| title_short | Antibiotikaresistens hos bakterier isolerade från friska hundar i Sverige : indikatorbakterier E. coli & Enterococcus spp samt Staphylococcus spp isolerade från hud |
| title_sort | antibiotikaresistens hos bakterier isolerade från friska hundar i sverige : indikatorbakterier e. coli & enterococcus spp samt staphylococcus spp isolerade från hud |
| topic | cephalosporin antimikrobiell resistens hund frisk hund antibiotika MRSA MRSI E. coli |