Development of a real-time PCR for in vivo diagnosis of feline Borna disease
Borna disease virus (BDV) is the causative agent of a neurological disorder in mammals and ostriches called Borna disease. BDV is also of interest as infective agent in humans with psychiatric diseases, such as mood disorders and schizophrenia, as well as in experimental models of these diseases. In...
| Autor principal: | |
|---|---|
| Formato: | L3 |
| Lenguaje: | Inglés sueco |
| Publicado: |
SLU/Dept. of Molecular Biosciences
2004
|
| Materias: |
| _version_ | 1855571827162611712 |
|---|---|
| author | Johansson, Jonas |
| author_browse | Johansson, Jonas |
| author_facet | Johansson, Jonas |
| author_sort | Johansson, Jonas |
| collection | Epsilon Archive for Student Projects |
| description | Borna disease virus (BDV) is the causative agent of a neurological disorder in mammals and ostriches called Borna disease. BDV is also of interest as infective agent in humans with psychiatric diseases, such as mood disorders and schizophrenia, as well as in experimental models of these diseases. In Sweden, the progressive and mostly fatal neurological disorder among domestic cats, known as staggering disease or feline Borna disease, is caused by BDV. Except for cats, natural BDV infection has also been described in horses and a free-ranging lynx in Sweden. Major clinical signs of staggering disease are staggering movement, alteration in behaviour, lumbosacral pain and inability to retract the claws. Today staggering disease is clinically suspected by excluding other reasons for clinical signs and the diagnosis is further established by examining the central nervous system histopathologically at autopsy. The diagnosis is finally confirmed by immunohistochemistry. In order to get a better clinical diagnostic tool, a duplex real-time polymerase chain reaction (PCR) assay was developed. This assay has been proven positive with different strains of BDV in infected cell lines and brain tissue samples of experimentally infected cats. Further evaluation of the new assay has to be done, such as testing the sensitivity and finding the best type of sample from the living patient. When the diagnostic method is finally evaluated it will also be essential in the evaluation of new therapies, as well as in epidemiological studies. |
| format | L3 |
| id | RepoSLU11199 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | SLU/Dept. of Molecular Biosciences |
| publisherStr | SLU/Dept. of Molecular Biosciences |
| record_format | eprints |
| spelling | RepoSLU111992017-09-26T08:33:52Z Development of a real-time PCR for in vivo diagnosis of feline Borna disease Utveckling av en realtids-PCR för in vivo-diagnostik vid vingelsjuka hos katt Johansson, Jonas Borna disease virus BDV Borna disease staggering disease real-time PCR feline neurological disorder Borna disease virus (BDV) is the causative agent of a neurological disorder in mammals and ostriches called Borna disease. BDV is also of interest as infective agent in humans with psychiatric diseases, such as mood disorders and schizophrenia, as well as in experimental models of these diseases. In Sweden, the progressive and mostly fatal neurological disorder among domestic cats, known as staggering disease or feline Borna disease, is caused by BDV. Except for cats, natural BDV infection has also been described in horses and a free-ranging lynx in Sweden. Major clinical signs of staggering disease are staggering movement, alteration in behaviour, lumbosacral pain and inability to retract the claws. Today staggering disease is clinically suspected by excluding other reasons for clinical signs and the diagnosis is further established by examining the central nervous system histopathologically at autopsy. The diagnosis is finally confirmed by immunohistochemistry. In order to get a better clinical diagnostic tool, a duplex real-time polymerase chain reaction (PCR) assay was developed. This assay has been proven positive with different strains of BDV in infected cell lines and brain tissue samples of experimentally infected cats. Further evaluation of the new assay has to be done, such as testing the sensitivity and finding the best type of sample from the living patient. When the diagnostic method is finally evaluated it will also be essential in the evaluation of new therapies, as well as in epidemiological studies. Bornavirus (Borna disease virus, BDV) orsakar en neurologisk sjukdom, som kallas bornasjuka, hos flera däggdjur och struts. BDV har också visat sig vara intressant vid vissa psykiatriska sjukdomar hos människa, såsom depression och schizofreni, eftersom virus och antikroppar har påvisats hos patienter med sådana sjukdomar. Dessutom är experimentellt infekterade försöksdjur intressanta som modeller för psykiatrisk sjukdom. I Sverige förekommer en progressiv och ofta fatal neurologisk sjukdom hos katt, som kallas för vingelsjuka. Denna orsakas av BDV. Förutom hos katter har naturlig bornavirusinfektion i Sverige även beskrivits hos hästar och ett lodjur med neurologiska symtom. Framstående symtom på vingelsjuka är vinglig gång (bakbensataxi), beteendeförändringar, smärta från länd- och korsrygg samt oförmåga att dra in klorna, framför allt på bakbenen. Idag misstänks den kliniska diagnosen genom att utesluta övriga orsaker till symtom och diagnosen kan inte styrkas förrän vid en histopatologisk undersökning av hjärna, hjärnhinnor och ryggmärg efter obduktion. Diagnos kan slutligen fastställas vid immunhistokemi. För att få en möjlighet att ställa diagnos hos den levande katten har en realtids-PCR utvecklats. Denna har visat sig vara positiv vid ett test där olika virusstammar användes, både från infekterade celler och hjärnvävnad från experimentellt infekterade katter. Fortfarande behöver flera studier göras innan den kan användas kliniskt. Känsligheten ska testas och även vilket typ av provmaterial från den levande patienten som innehåller mycket virus. När diagnostiken är utprovad kommer den även vara ett användbart verktyg vid utveckling av nya behandlingar och vidare studier av sjukdomens epidemiologi. SLU/Dept. of Molecular Biosciences 2004 L3 eng swe https://stud.epsilon.slu.se/11199/ |
| spellingShingle | Borna disease virus BDV Borna disease staggering disease real-time PCR feline neurological disorder Johansson, Jonas Development of a real-time PCR for in vivo diagnosis of feline Borna disease |
| title | Development of a real-time PCR for in vivo diagnosis of feline Borna disease |
| title_full | Development of a real-time PCR for in vivo diagnosis of feline Borna disease |
| title_fullStr | Development of a real-time PCR for in vivo diagnosis of feline Borna disease |
| title_full_unstemmed | Development of a real-time PCR for in vivo diagnosis of feline Borna disease |
| title_short | Development of a real-time PCR for in vivo diagnosis of feline Borna disease |
| title_sort | development of a real-time pcr for in vivo diagnosis of feline borna disease |
| topic | Borna disease virus BDV Borna disease staggering disease real-time PCR feline neurological disorder |