Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica
Infectious Bronchitis and Infectious Bursal Disease, also called Gumboro Disease, are two important poultry diseases, causing serious illness in chickens and major production losses. In Costa Rica, as in many other developing countries, poultry production is an important industry and their products...
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| Formato: | L3 |
| Lenguaje: | Inglés sueco |
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SLU/Dept. of Microbiology
2004
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| Materias: |
| _version_ | 1855571824938582016 |
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| author | Lindahl, Johanna |
| author_browse | Lindahl, Johanna |
| author_facet | Lindahl, Johanna |
| author_sort | Lindahl, Johanna |
| collection | Epsilon Archive for Student Projects |
| description | Infectious Bronchitis and Infectious Bursal Disease, also called Gumboro Disease, are two important poultry diseases, causing serious illness in chickens and major production losses. In Costa Rica, as in many other developing countries, poultry production is an important industry and their products are important parts of every day food supply.
During ten weeks the author was working at the laboratory of virology at Universidad Nacional in Costa Rica. Three viral isolates of Infectious Bronchitis Virus, IBV, were characterized by Restriction Fragment Length Polymorphism (RFLP), one isolate was found to be a Massachusetts strain and the two others a new Costa Rican variant the IBV-CR-53 strain. Sera from 182 wild birds, sampled between 2000 and 2002, were investigated for presence of antibodies to IBV using a blocking ELISA. Two pigeons of the species Zenaida asiatica were positive and two pigeons of the species Columba fasciata were suspected positive. Two commercial ELISA kits were compared and the results were similar.
Seven samples from macerated bursas and cell cultures from suspected cases of Infectious Bursal Disease, IBD, were characterized using a antigen-capture ELISA and were shown to be classical strains of IBD virus. In virus neutralization test using chicken sera sampled at three, ten and 18 days of age it was shown that the neutralizing antibody titers were significantly lower against a wild type strain than against a vaccine strain and that the half-life of antibodies was 2.5 days rendering the chickens with little protection at 18 days of age.
A Costa Rican chicken production plant was visited in order to view typical premises. The pens had open net walls and thus some problems with biosecurity. Blood samples sent to the laboratory for routine antibody control showed that in spite of vaccine strategies, protection against IBV was low at 44 days of age. |
| format | L3 |
| id | RepoSLU11189 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés swe |
| publishDate | 2004 |
| publishDateSort | 2004 |
| publisher | SLU/Dept. of Microbiology |
| publisherStr | SLU/Dept. of Microbiology |
| record_format | eprints |
| spelling | RepoSLU111892017-09-26T09:00:21Z Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica Infektiöst bronkitvirus och infektiöst bursitvirus : en studie vid Universidad Nacional i Costa Rica Lindahl, Johanna Costa Rica poultry gumboro infectious bronchitis virus IBV infectious bursal disease virus IBDV Universidad Nacional avian medicine avian virology Infectious Bronchitis and Infectious Bursal Disease, also called Gumboro Disease, are two important poultry diseases, causing serious illness in chickens and major production losses. In Costa Rica, as in many other developing countries, poultry production is an important industry and their products are important parts of every day food supply. During ten weeks the author was working at the laboratory of virology at Universidad Nacional in Costa Rica. Three viral isolates of Infectious Bronchitis Virus, IBV, were characterized by Restriction Fragment Length Polymorphism (RFLP), one isolate was found to be a Massachusetts strain and the two others a new Costa Rican variant the IBV-CR-53 strain. Sera from 182 wild birds, sampled between 2000 and 2002, were investigated for presence of antibodies to IBV using a blocking ELISA. Two pigeons of the species Zenaida asiatica were positive and two pigeons of the species Columba fasciata were suspected positive. Two commercial ELISA kits were compared and the results were similar. Seven samples from macerated bursas and cell cultures from suspected cases of Infectious Bursal Disease, IBD, were characterized using a antigen-capture ELISA and were shown to be classical strains of IBD virus. In virus neutralization test using chicken sera sampled at three, ten and 18 days of age it was shown that the neutralizing antibody titers were significantly lower against a wild type strain than against a vaccine strain and that the half-life of antibodies was 2.5 days rendering the chickens with little protection at 18 days of age. A Costa Rican chicken production plant was visited in order to view typical premises. The pens had open net walls and thus some problems with biosecurity. Blood samples sent to the laboratory for routine antibody control showed that in spite of vaccine strategies, protection against IBV was low at 44 days of age. Infektiös Bronkit och Infektiös Bursit, eller Gumborosjuka, är två mycket betydelsefulla fjäderfäsjukdomar. De orsakar allvarliga sjukdomsutbrott och ekonomiska förluster. I Costa Rica är fjäderfäproduktion en viktig industri som inte bara ger arbetstillfällen och uppehälle utan även mat till befolkningen. Under tio veckor hade författaren möjlighet att arbeta på virologilaboratoriet vid Universidad Nacional i Costa Rica för att studera dessa virus närmare. Tre isolat av IBV karaktäriserades genom RFLP-teknik. En befanns vara en Massachusetts stam och de andra två en ny Costa Ricansk variant med benämningen IBV-CR53. Dessutom jämfördes två kommerciella ELISA-kit; en blocking och en indirekt ELISA. De båda kiten befanns vara jämförbara på flocknivå. Serum från 182 vilda fåglar som provtagits år 2000 och framåt testades för prevalens av antikroppar mot IBV. Två duvor av arten Zenaida asiatica visade sig vara positiva och två Columba fasciata misstänkt positiva. Sju prover från misstänkta Gumborofall undersöktes med en DAS-ELISA och alla var klassiska stammar. Genom en virusneutralisations test jämfördes serum från kycklingar provtagna vid tre, tio och 18 dagars ålder för att se hur det maternella antikroppsskyddet minskade. Den neutraliserande effekten jämfördes mellan en vaccinstam och en virusstam som isolerats vid ett sjukdomsutbrott och visade sig vara signifikant lägre mot det senare viruset. Halveringstiden för antikropparna beräknades till 2.5 dagar och titrarna var mycket låga vid 18 dagars ålder. En Costa Ricansk fjäderfäanläggning besöktes för att studera typiska förhållanden. Byggnaderna som hönsen hölls i var nättäckta vilket förklarar en del av problemen som finns med att upprätthålla adekvat smittskydd. Blodprov som inskickats för antikroppsanalys visade dessutom att kycklingarna vid 44 dagars ålder hade mycket låga halter antikroppar mot IBV. SLU/Dept. of Microbiology 2004 L3 eng swe https://stud.epsilon.slu.se/11189/ |
| spellingShingle | Costa Rica poultry gumboro infectious bronchitis virus IBV infectious bursal disease virus IBDV Universidad Nacional avian medicine avian virology Lindahl, Johanna Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica |
| title | Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica |
| title_full | Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica |
| title_fullStr | Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica |
| title_full_unstemmed | Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica |
| title_short | Infectious bronchitis virus and infectious bursal disease virus : a study performed at Universidad Nacional of Costa Rica |
| title_sort | infectious bronchitis virus and infectious bursal disease virus : a study performed at universidad nacional of costa rica |
| topic | Costa Rica poultry gumboro infectious bronchitis virus IBV infectious bursal disease virus IBDV Universidad Nacional avian medicine avian virology |