Opportunistic pathogenic filamentous fungal species in health supplements
Invasive fungal infections (IFI) are a threat to individuals with a suppressed immune system and the rate of IFI cases in the world is increasing. These types of infections occur both at home and during hospitalization. At hospitals, small wounds caused by needles may later be infected with conidia,...
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| Formato: | Second cycle, A2E |
| Lenguaje: | sueco Inglés |
| Publicado: |
2017
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| Acceso en línea: | https://stud.epsilon.slu.se/10487/ |
| _version_ | 1855571699228999680 |
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| author | Arzoomand, Attal |
| author_browse | Arzoomand, Attal |
| author_facet | Arzoomand, Attal |
| author_sort | Arzoomand, Attal |
| collection | Epsilon Archive for Student Projects |
| description | Invasive fungal infections (IFI) are a threat to individuals with a suppressed immune system and the rate of IFI cases in the world is increasing. These types of infections occur both at home and during hospitalization. At hospitals, small wounds caused by needles may later be infected with conidia, air-borne opportunistic pathogenic conidia may be inhaled, and contaminated food may be consumed. IFI in the gastrointestinal tract increases the mortality rate due to late detection of those infections. The objective of this study was to investigate if food products that immune suppressed individuals would prefer to buy, or products marketed toward those individuals, would contain any opportunistic pathogenic filamentous fungi. The study also compares if any difference can be found between samples incubated at the temperatures of 25 ºC and 35 ºC, this to simplify any screening of filamentous fungi. Among 16 different products (1 tea, 8 probiotics and 7 plant based products), filamentous fungi were only detected in two leaf powder products, one product of chia seeds, camomile tea, two probiotics and two fruit and vegetable products. The two opportunistic pathogenic species Rhizopus oryzae and Mucor circinelloides were detected in one of the probiotic products incubated at 35 ºC. R. oryzae was also detected in chia seeds together with other opportunistic pathogens, such as Lichtheimia ramosa and Aspergillus flavus when incubated at 35 ºC. Both A. flavus and A. fumigatus was detected in nettle leaf powder and fruit powder incubated at 35 ºC. The fruit powder did however also contain the opportunistic pathogen Byssochlamys spectabilis, and the nettle leaf powder did also contain the opportunistic pathogen R. microsporus. Leaf powder made of Moringa oleifera was the only product that was found to be heavily contaminated with L. ramosa. The study could show that there is a slightly better chance to reduce the growth of non-opportunistic pathogenic species and target only the opportunistic pathogenic species by incubating samples at 35 ºC. To completely avoid growth of non-opportunistic pathogenic species is not possible through this method only. |
| format | Second cycle, A2E |
| id | RepoSLU10487 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2017 |
| publishDateSort | 2017 |
| record_format | eprints |
| spelling | RepoSLU104872017-10-19T10:04:26Z https://stud.epsilon.slu.se/10487/ Opportunistic pathogenic filamentous fungal species in health supplements Arzoomand, Attal Food science and technology Invasive fungal infections (IFI) are a threat to individuals with a suppressed immune system and the rate of IFI cases in the world is increasing. These types of infections occur both at home and during hospitalization. At hospitals, small wounds caused by needles may later be infected with conidia, air-borne opportunistic pathogenic conidia may be inhaled, and contaminated food may be consumed. IFI in the gastrointestinal tract increases the mortality rate due to late detection of those infections. The objective of this study was to investigate if food products that immune suppressed individuals would prefer to buy, or products marketed toward those individuals, would contain any opportunistic pathogenic filamentous fungi. The study also compares if any difference can be found between samples incubated at the temperatures of 25 ºC and 35 ºC, this to simplify any screening of filamentous fungi. Among 16 different products (1 tea, 8 probiotics and 7 plant based products), filamentous fungi were only detected in two leaf powder products, one product of chia seeds, camomile tea, two probiotics and two fruit and vegetable products. The two opportunistic pathogenic species Rhizopus oryzae and Mucor circinelloides were detected in one of the probiotic products incubated at 35 ºC. R. oryzae was also detected in chia seeds together with other opportunistic pathogens, such as Lichtheimia ramosa and Aspergillus flavus when incubated at 35 ºC. Both A. flavus and A. fumigatus was detected in nettle leaf powder and fruit powder incubated at 35 ºC. The fruit powder did however also contain the opportunistic pathogen Byssochlamys spectabilis, and the nettle leaf powder did also contain the opportunistic pathogen R. microsporus. Leaf powder made of Moringa oleifera was the only product that was found to be heavily contaminated with L. ramosa. The study could show that there is a slightly better chance to reduce the growth of non-opportunistic pathogenic species and target only the opportunistic pathogenic species by incubating samples at 35 ºC. To completely avoid growth of non-opportunistic pathogenic species is not possible through this method only. Mögelinfektion hos immunförsvagade patienter är en livshotande fara, och har visats ha en ökande trend i världen. Mögelinfektioner kan förekomma i hemmet och på sjukhuset via hål i huden från nålar som sedan kontamineras av mögelsporer, inandning av sporer i luften eller förtäring av kontaminerade livsmedel. Mögelinfektioner i mag- och tarmkanalen ger en risk för dödlig utgång på grund av att den ofta undkommer att identifieras. Syftet med denna studie var att undersöka om livsmedelsprodukter som personer med försvagat immunförsvar skulle tänka söka sig till, eller de produkter som marknadsför sig mot dessa individer, kan innehålla potentiella patogena mögelarter. Studien ska också jämföra om det förekommer några skillnader i mögeltillväxt av olika arter i prover som inkuberas i 25 ºC eller 35 ºC för att förenkla identifieringen av olika potentiella patogena mögelarter. Bland 16 olika livsmedelsprodukter (1 té. 8 probiotika och 7 produkter baserade på frukt och grönsaker) isolerades mögel från två bladpulversprodukter, chiafrön, kamomill té, två probiotikaprodukter och två frukt- och grönsakspulvriserade produkter. De potentiella patogena mögelsvamparna Rhizopus oryzae och Mucor circinelloides var påvisad i en av probiotikaproverna som inkuberades i 35 ºC. Förutom i probiotika fanns även R. oryae, bland de potentiella patogena arterna Lichtheimia ramosa och Aspergillus flavus i chiafrön inkuberade i 35 ºC. Både A. flavus och A. fumigatus kunde påvisas i både bladpulver från nässla och i fruktpulver inkuberade i 35 ºC. Fruktpulvret visades även innehålla spår av den potentiella patogena mögelarten Byssochlamys spectabilis, och nässelbladpulvret påvisades ha spår av R. microsporus. Bladpulver gjord av Moringa oleifera var den enda produkten som visades innehålla flest kolonier av mögelarten L. ramosa. Studien kunde visa att vissa oönskade icke-potentiella patogena mögelarter minskade på de plattor som inkuberades i 35 ºC. Men att nå en fullständig isolering av endast potentiella patogena mögelarter och undgå andra icke-potentiella patogena mögelarter går inte att genomföra med endast denna metod. 2017-07-14 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/10487/1/arzoomand_a_171019.pdf Arzoomand, Attal, 2017. Opportunistic pathogenic filamentous fungal species in health supplements : potential risk to immune compromised individuals. Second cycle, A2E. Uppsala: (NL, NJ) > Department of Molecular Sciences <https://stud.epsilon.slu.se/view/divisions/OID-425.html> urn:nbn:se:slu:epsilon-s-6798 eng |
| spellingShingle | Food science and technology Arzoomand, Attal Opportunistic pathogenic filamentous fungal species in health supplements |
| title | Opportunistic pathogenic filamentous fungal species in health supplements |
| title_full | Opportunistic pathogenic filamentous fungal species in health supplements |
| title_fullStr | Opportunistic pathogenic filamentous fungal species in health supplements |
| title_full_unstemmed | Opportunistic pathogenic filamentous fungal species in health supplements |
| title_short | Opportunistic pathogenic filamentous fungal species in health supplements |
| title_sort | opportunistic pathogenic filamentous fungal species in health supplements |
| topic | Food science and technology |
| url | https://stud.epsilon.slu.se/10487/ https://stud.epsilon.slu.se/10487/ |