Preoperative training of pigs used for kidney transplantation research
The use of pigs as a preclinical model has increased dramatically the last decades. The specie´s unique anatomy and physiological features make it an appropriate transplantation study model for humans. In human medicine, kidney transplants have been established as the best and most cost-effective tr...
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| Formato: | Second cycle, A2E |
| Lenguaje: | Inglés Inglés |
| Publicado: |
2017
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| Acceso en línea: | https://stud.epsilon.slu.se/10233/ |
| _version_ | 1855571658885038080 |
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| author | Rydén, Anneli |
| author_browse | Rydén, Anneli |
| author_facet | Rydén, Anneli |
| author_sort | Rydén, Anneli |
| collection | Epsilon Archive for Student Projects |
| description | The use of pigs as a preclinical model has increased dramatically the last decades. The specie´s unique anatomy and physiological features make it an appropriate transplantation study model for humans. In human medicine, kidney transplants have been established as the best and most cost-effective treatment for people with end-stage renal failure. To improve the outcome of renal transplantation in humans, the graft survival rate needs to be improved and an animal model is essential for this research. However, information regarding preoperative training to allow for stress- and pain-free repeated blood sampling as well as blood sampling techniques and measurement of the urine volume postoperatively to kidney transplantation is limited.
Eight Swedish high-health domestic pigs were included in a training program in which touching and brushing the ears, ultrasound of the urinary bladder and physical examinations were performed for 15 minutes per pig every day for two weeks before transplantation surgery. Six of eight pigs underwent kidney transplantation and insertion of a catheter in the auricular vein with the Seldinger technique. After surgery, the pigs were kept for five days and blood sampling and ultrasound of the urinary bladder were performed daily. The effect of the preoperative training on postoperative examinations were evaluated.
The transplantation surgery and the anaesthesia were successful and the pigs recovered well after surgery. The training period of two weeks was sufficient to enable blood sampling and ultrasound examination of the urinary bladder without restrain. Furthermore, placement of a catheter with the Seldinger technique in the auricular vein was successful and withdrawal of blood was possible in four out of six pigs for five days post-surgery. In conclusion this study shows that the pig can be trained preoperatively to accept interventions and measurements postoperatively, which makes the pig a suitable animal model in transplantation studies. |
| format | Second cycle, A2E |
| id | RepoSLU10233 |
| institution | Swedish University of Agricultural Sciences |
| language | Inglés Inglés |
| publishDate | 2017 |
| publishDateSort | 2017 |
| record_format | eprints |
| spelling | RepoSLU102332018-09-20T23:15:05Z https://stud.epsilon.slu.se/10233/ Preoperative training of pigs used for kidney transplantation research Rydén, Anneli Veterinary science and hygiene - General aspects The use of pigs as a preclinical model has increased dramatically the last decades. The specie´s unique anatomy and physiological features make it an appropriate transplantation study model for humans. In human medicine, kidney transplants have been established as the best and most cost-effective treatment for people with end-stage renal failure. To improve the outcome of renal transplantation in humans, the graft survival rate needs to be improved and an animal model is essential for this research. However, information regarding preoperative training to allow for stress- and pain-free repeated blood sampling as well as blood sampling techniques and measurement of the urine volume postoperatively to kidney transplantation is limited. Eight Swedish high-health domestic pigs were included in a training program in which touching and brushing the ears, ultrasound of the urinary bladder and physical examinations were performed for 15 minutes per pig every day for two weeks before transplantation surgery. Six of eight pigs underwent kidney transplantation and insertion of a catheter in the auricular vein with the Seldinger technique. After surgery, the pigs were kept for five days and blood sampling and ultrasound of the urinary bladder were performed daily. The effect of the preoperative training on postoperative examinations were evaluated. The transplantation surgery and the anaesthesia were successful and the pigs recovered well after surgery. The training period of two weeks was sufficient to enable blood sampling and ultrasound examination of the urinary bladder without restrain. Furthermore, placement of a catheter with the Seldinger technique in the auricular vein was successful and withdrawal of blood was possible in four out of six pigs for five days post-surgery. In conclusion this study shows that the pig can be trained preoperatively to accept interventions and measurements postoperatively, which makes the pig a suitable animal model in transplantation studies. Nyttjande av grisen som preklinisk modell har ökat markant de senaste decennierna. Djurslagets unika anatomi och fysiologi gör den till en lämplig modell för människan i transplantationsstudier. Inom humanmedicinen har njurtransplantationer blivit den bästa och mest kostnadseffektiva behandlingen för människor som drabbats av njursvikt. För att resultatet efter transplantationen ska kunna förbättras, behöver överlevnaden av transplantatet bli bättre. I dessa studier är en djurmodell oumbärlig. Information gällande preoperativ träning för att kunna utföra stress- och smärtfri upprepad blodprovs tagning och mätning av urinvolymen postoperativt till njurtransplantation är begränsad. Åtta svenska SPF grisar var inkluderade i ett träningsprogram där t.ex. klappa och borsta öronen, ultraljudsundersökning av urinblåsan och klinisk undersökning tränades i 15 minuter per gris, varje dag, i två veckor före transplantationskirurgi. Sex av åtta grisar genomgick njurtransplantation och kateterisering i auricularvenen med Seldingers teknik. Efter kirurgin utfördes blodprovstagning och ultraljudsundersökning av urinblåsan varje dag under fem dagar. Effekten av den preoperativa träningen på de postoperativa interventionerna utvärderades. Transplantationskirurgin och anestesin förlöpte utmärkt och grisarna återhämtade sig bra efter kirurgin. Den två veckor preoperativa träningen var tillräcklig för att möjliggöra blodprovstagning och ultraljudsundersökning av urinblåsan postoperativt utan stress för djuren. Därutöver var kateteriseringen i auricularvenen med Seldingers teknik tillfredställande och blodprovstagning var möjlig i fyra av sex grisar i fem dagar efter kirurgin. Resultatet visar att grisar kan tränas preoperativt för att acceptera interventioner och mätningar postoperativt, vilket gör grisen till en lämplig djurmodell i transplantationsstudier 2017-06-07 Second cycle, A2E NonPeerReviewed application/pdf en https://stud.epsilon.slu.se/10233/1/Ryden_A_170405.pdf Rydén, Anneli, 2017. Preoperative training of pigs used for kidney transplantation research : refinement of postoperative procedure. Second cycle, A2E. Uppsala: (VH) > Dept. of Biomedical Sciences and Veterinary Public Health (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-713.html> urn:nbn:se:slu:epsilon-s-6429 eng |
| spellingShingle | Veterinary science and hygiene - General aspects Rydén, Anneli Preoperative training of pigs used for kidney transplantation research |
| title | Preoperative training of pigs used for kidney transplantation research |
| title_full | Preoperative training of pigs used for kidney transplantation research |
| title_fullStr | Preoperative training of pigs used for kidney transplantation research |
| title_full_unstemmed | Preoperative training of pigs used for kidney transplantation research |
| title_short | Preoperative training of pigs used for kidney transplantation research |
| title_sort | preoperative training of pigs used for kidney transplantation research |
| topic | Veterinary science and hygiene - General aspects |
| url | https://stud.epsilon.slu.se/10233/ https://stud.epsilon.slu.se/10233/ |