Effect of freezing and thawing rates on camel sperm quality
The objective of this study was to evaluate the effect of different freezing rates and thawing temperatures on the quality of camel spermatozoa post thawing. Ten ejaculates from five male camels were frozen at five different freezing rates, achieved by placing the straws at specific heights above...
| Main Author: | |
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| Format: | Second cycle, A2E |
| Language: | Swedish Inglés |
| Published: |
2017
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| Subjects: | |
| Online Access: | https://stud.epsilon.slu.se/10136/ |
| _version_ | 1855571643700609024 |
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| author | Elwing, Bodil |
| author_browse | Elwing, Bodil |
| author_facet | Elwing, Bodil |
| author_sort | Elwing, Bodil |
| collection | Epsilon Archive for Student Projects |
| description | The objective of this study was to evaluate the effect of different freezing rates and thawing
temperatures on the quality of camel spermatozoa post thawing. Ten ejaculates from five male
camels were frozen at five different freezing rates, achieved by placing the straws at specific
heights above the surface of liquid nitrogen for different times (4 cm for 15 min; 1 cm for 15
min; 7 cm for 15 min; 7 cm for 5 min + 4 cm for 3 min; 4 cm for 5 min + 1 cm for 3 min)
followed by storage in liquid nitrogen. Two thawing temperatures (60°C for 10 sec or 37° for
30 sec) were subsequently tested. The sperm samples were evaluated for total and progressive
motility, kinematics, membrane and acrosome integrity and membrane functionality zero, one
and two hours post thawing. Camel sperm showed a high tolerance for freezing rates with small
differences between the different freezing rates. Significantly (p<0.05) better values for sperm
characteristics were obtained from the fastest freezing rate compared to a slower rate for total
and progressive motility and some kinematic parameters, but no treatment was found to be
significantly better for all parameters of sperm quality. However, post thaw sperm quality was
better with the faster thawing rate compared to the lower rate. No interactions between freezing
and thawing rates were found in the study. In conclusion, according to this study, a fast freezing
rate may be more beneficial for camel spermatozoa than slower freezing rates, and the thawing
should be conducted at 60°C. |
| format | Second cycle, A2E |
| id | RepoSLU10136 |
| institution | Swedish University of Agricultural Sciences |
| language | Swedish Inglés |
| publishDate | 2017 |
| publishDateSort | 2017 |
| record_format | eprints |
| spelling | RepoSLU101362017-04-24T11:37:16Z https://stud.epsilon.slu.se/10136/ Effect of freezing and thawing rates on camel sperm quality Elwing, Bodil Veterinary science and hygiene - General aspects The objective of this study was to evaluate the effect of different freezing rates and thawing temperatures on the quality of camel spermatozoa post thawing. Ten ejaculates from five male camels were frozen at five different freezing rates, achieved by placing the straws at specific heights above the surface of liquid nitrogen for different times (4 cm for 15 min; 1 cm for 15 min; 7 cm for 15 min; 7 cm for 5 min + 4 cm for 3 min; 4 cm for 5 min + 1 cm for 3 min) followed by storage in liquid nitrogen. Two thawing temperatures (60°C for 10 sec or 37° for 30 sec) were subsequently tested. The sperm samples were evaluated for total and progressive motility, kinematics, membrane and acrosome integrity and membrane functionality zero, one and two hours post thawing. Camel sperm showed a high tolerance for freezing rates with small differences between the different freezing rates. Significantly (p<0.05) better values for sperm characteristics were obtained from the fastest freezing rate compared to a slower rate for total and progressive motility and some kinematic parameters, but no treatment was found to be significantly better for all parameters of sperm quality. However, post thaw sperm quality was better with the faster thawing rate compared to the lower rate. No interactions between freezing and thawing rates were found in the study. In conclusion, according to this study, a fast freezing rate may be more beneficial for camel spermatozoa than slower freezing rates, and the thawing should be conducted at 60°C. Syftet med denna studien var att utvärdera om olika frysningsgrader och upptiningstemperaturer kan påverka kvaliteten hos kryokonserverad kamelsperma efter upptining. Tio ejakulat från fem olika hanar frystes i fem olika hastigheter, hastigheterna bestämdes av avståndet från provet till ytan av flytande kväve (4 cm i 15 min; 1 cm i 15 min; 7 cm i 15 min; 7 cm i 5 min + 4 cm i 3 min; 4 cm i 5 min + 1 cm i 3 min) och kryokonserverades sedan. Två olika upptiningstemperaturer (60°C i 10 sekunder eller 37°C i 30 sekunder) utvärderades sedan. Spermierna evaluerades med avseende på total och progressiv motilitet, kinematiska parametrar, membran- och akrosomintegritet och membranfunktionalitet noll, en och två timmar efter upptining. Kamelsperma visade hög tolerans för olika frysninghastigheter, då det var små skillnader i resultat mellan de olika behandlingarna. Signifikant (p<0,05) bättre värden för spermieegenskaper erhölls från den snabbaste frysningshastigheten i jämförelse med en lägre hastighet med avseende på total och progressiv motilitet samt några kinematiska parametrar, men ingen frysningshastighet var signifikant bättre än alla andra hastigheter för någon parameter. Gällande upptiningstemperaturer visade snabb upptining (60°C i 10 sekunder) bättre resultat jämfört med lägre temperatur (37°C i 30 sekunder). Inga interaktioner mellan frys- och upptiningshastigheter påträffades i studien. Sammanfattningsvis, enligt denna studie, kan en snabb nedfrysningshastighet vara fördelaktig för kamelsperma, och upptining av kamelsperma bör utföras vid 60 °C. 2017-04-11 Second cycle, A2E NonPeerReviewed application/pdf sv https://stud.epsilon.slu.se/10136/1/elwing_b_20170424.pdf Elwing, Bodil, 2017. Effect of freezing and thawing rates on camel sperm quality. Second cycle, A2E. Uppsala: (VH) > Dept. of Clinical Sciences (until 231231) <https://stud.epsilon.slu.se/view/divisions/OID-715.html> urn:nbn:se:slu:epsilon-s-6356 eng |
| spellingShingle | Veterinary science and hygiene - General aspects Elwing, Bodil Effect of freezing and thawing rates on camel sperm quality |
| title | Effect of freezing and thawing rates on camel sperm quality |
| title_full | Effect of freezing and thawing rates on camel sperm quality |
| title_fullStr | Effect of freezing and thawing rates on camel sperm quality |
| title_full_unstemmed | Effect of freezing and thawing rates on camel sperm quality |
| title_short | Effect of freezing and thawing rates on camel sperm quality |
| title_sort | effect of freezing and thawing rates on camel sperm quality |
| topic | Veterinary science and hygiene - General aspects |
| url | https://stud.epsilon.slu.se/10136/ https://stud.epsilon.slu.se/10136/ |