Shoot regeneration from peduncles and shoot-like regeneration from leaves of babaco, Carica pentagona

C. pentagona shoots were regenerated from peduncle sections cultured on medium containing benzyladenine (BA, 0, 0.5 or 1.0 mg/litre) plus IAA (0.5 or 1.0 mg/litre) under a 16-h or 18-h photoperiod and a light intensity of 16 micro mol-1 m-2. Whole leaves from in vitro-stock material and leaf section...

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Detalles Bibliográficos
Autores principales: Vega de Rojas, R, Barberan, C, Kitto, S.L
Formato: Artículo
Lenguaje:Inglés
Publicado: IICA, San José (Costa Rica) 2020
Materias:
AIA
Acceso en línea:https://repositorio.catie.ac.cr/handle/11554/9635
Descripción
Sumario:C. pentagona shoots were regenerated from peduncle sections cultured on medium containing benzyladenine (BA, 0, 0.5 or 1.0 mg/litre) plus IAA (0.5 or 1.0 mg/litre) under a 16-h or 18-h photoperiod and a light intensity of 16 micro mol-1 m-2. Whole leaves from in vitro-stock material and leaf sections containing either a petiole or main vein from greenhouse plants regenerated roots and/or shoot-like structures on medium containing BA (1.0 or 1.5 mg/litre) plus IAA (1.0 or 3.0 mg/litre). Histological analysis of sections of peduncles and leaves demonstrated that shoots appeared to regenerate directly from cells associated with the vascular systems.