Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR

A large collection of coffee genetic resources is conserved in Costa Rica. In this study, microsatellite DNA fingerprinting of coffee through singleplex and multiplex PCR approaches coupled with capillary electrophoresis are described. To validate both methods, germplasm of Coffea spp. (Arabica and...

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Main Authors:	Sánchez, Elodia, Solano, William, Gatica-Arias, Andrés, Chavarría, Max, Araya-Valverde, Emanuel
Format:	Artículo
Language:	Inglés
Published:	Crop Breeding and Applied Biotechnology 2020
Online Access:	https://repositorio.catie.ac.cr/handle/11554/9461 http://dx.doi.org/10.1590/1984- 70332020v20n1a3

id	RepoCATIE9461
record_format	dspace
spelling	RepoCATIE94612023-11-16T16:30:05Z Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR Sánchez, Elodia Solano, William Gatica-Arias, Andrés Chavarría, Max Araya-Valverde, Emanuel A large collection of coffee genetic resources is conserved in Costa Rica. In this study, microsatellite DNA fingerprinting of coffee through singleplex and multiplex PCR approaches coupled with capillary electrophoresis are described. To validate both methods, germplasm of Coffea spp. (Arabica and non-Arabica) and intraspecific F1 hybrids were analyzed using fourteen microsatellite markers. It was observed that through both PCR methods the fingerprinting profile of a subset of samples was identical. The genetic analyses revealed that non-Arabica coffee displayed greater genetic variation than Arabica coffee did. In addition, microsatellite analyses allowed the separation of C. arabica from other species using the principal coordinate analysis (PCoA) approach. The neighbor-joining tree clustering analysis revealed either a grouping of wild genotypes separated from cultivars of C. arabica, or a relation of intraspecific F1 hybrids with parental lines. The utility of our methodology for the characterization of F1 hybrids not previously analyzed through SSR (Simple Sequence Repeats) fingerprinting is demonstrated. 2020-04-02T15:50:15Z 2020-04-02T15:50:15Z 2020 Artículo https://repositorio.catie.ac.cr/handle/11554/9461 http://dx.doi.org/10.1590/1984- 70332020v20n1a3 en Biotechnol info:eu-repo/semantics/openAccess application/pdf Crop Breeding and Applied Biotechnology
institution	Centro Agronómico Tropical de Investigación y Enseñanza
collection	Repositorio CATIE
language	Inglés
description	A large collection of coffee genetic resources is conserved in Costa Rica. In this study, microsatellite DNA fingerprinting of coffee through singleplex and multiplex PCR approaches coupled with capillary electrophoresis are described. To validate both methods, germplasm of Coffea spp. (Arabica and non-Arabica) and intraspecific F1 hybrids were analyzed using fourteen microsatellite markers. It was observed that through both PCR methods the fingerprinting profile of a subset of samples was identical. The genetic analyses revealed that non-Arabica coffee displayed greater genetic variation than Arabica coffee did. In addition, microsatellite analyses allowed the separation of C. arabica from other species using the principal coordinate analysis (PCoA) approach. The neighbor-joining tree clustering analysis revealed either a grouping of wild genotypes separated from cultivars of C. arabica, or a relation of intraspecific F1 hybrids with parental lines. The utility of our methodology for the characterization of F1 hybrids not previously analyzed through SSR (Simple Sequence Repeats) fingerprinting is demonstrated.
format	Artículo
author	Sánchez, Elodia Solano, William Gatica-Arias, Andrés Chavarría, Max Araya-Valverde, Emanuel
spellingShingle	Sánchez, Elodia Solano, William Gatica-Arias, Andrés Chavarría, Max Araya-Valverde, Emanuel Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR
author_facet	Sánchez, Elodia Solano, William Gatica-Arias, Andrés Chavarría, Max Araya-Valverde, Emanuel
author_sort	Sánchez, Elodia
title	Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR
title_short	Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR
title_full	Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR
title_fullStr	Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR
title_full_unstemmed	Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR
title_sort	microsatellite dna fingerprinting of coffea sp. germplasm conserved in costa rica through singleplex and multiplex pcr
publisher	Crop Breeding and Applied Biotechnology
publishDate	2020
url	https://repositorio.catie.ac.cr/handle/11554/9461 http://dx.doi.org/10.1590/1984- 70332020v20n1a3
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Microsatellite DNA fingerprinting of Coffea sp. germplasm conserved in Costa Rica through singleplex and multiplex PCR

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