Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris)
By treating the shoot apices of twenty young bean seedlings (Phaseolus vulgaris, 2n = 22) with 0.2% colchicine solution for 20 hours, an autotetraploid sector was obtained from a treated plant. Two seeds were produced from this sector, and the chromosome number of the radicles of these two seeds was...
| Autores principales: | , |
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| Formato: | Artículo |
| Lenguaje: | Español |
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Instituto Interamericano de Ciencias Agrícolas (IICA)
2025
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| Materias: | |
| Acceso en línea: | https://repositorio.catie.ac.cr/handle/11554/13732 |
| _version_ | 1854966417414160384 |
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| author | Monge Serrano, F. Moh, C.C. |
| author_browse | Moh, C.C. Monge Serrano, F. |
| author_facet | Monge Serrano, F. Moh, C.C. |
| author_sort | Monge Serrano, F. |
| collection | Repositorio CATIE |
| description | By treating the shoot apices of twenty young bean seedlings (Phaseolus vulgaris, 2n = 22) with 0.2% colchicine solution for 20 hours, an autotetraploid sector was obtained from a treated plant. Two seeds were produced from this sector, and the chromosome number of the radicles of these two seeds was cytologically identified as 4n = 44.
As compared with the diploid, some degree of gigantism was observed in these two 4n plants. The plants, the leaves, the stomata and the pollen grains were larger than those of the diploid. The 4n plants also had a prolonged reproductive period. About 50% of the pollen grains of the 4n plant showed intense staining with lactophenol-anilin blue, while almost 100% of the pollen grains of the 2n plants showed intense staining.
No cells with more than 10 or less than 5 quadrivalents were observed in the pollen mother cells (PMC) in the 4n plants. The average number of quadrivalents per PMC was 7.3 which indicates that an average of 66% of the chromosomes form quadrivalents.
The formation of bivalents in the 4n plants occurred in pairs from groups of the four homologues. This explains the high frequency of PMCs with even numbers of bivalents. The frequency of PMCs with univalents was of the same order as that of PMCs with trivalents, suggesting that in the majority of cases, the production of a univalent was due to the formation of a trivalent from the four homologues. |
| format | Artículo |
| id | RepoCATIE13732 |
| institution | Centro Agronómico Tropical de Investigación y Enseñanza |
| language | Español |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Instituto Interamericano de Ciencias Agrícolas (IICA) |
| publisherStr | Instituto Interamericano de Ciencias Agrícolas (IICA) |
| record_format | dspace |
| spelling | RepoCATIE137322025-08-23T00:12:35Z Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris) Monge Serrano, F. Moh, C.C. Estudio biológico||biological surveys||undefined||enquête biologique Citología||cytology||Citologia||cytologie Planta||plants||planta||plante Autotetraploidía||autotetraploidy||autotetraploidia||autotétraploïdie Fríjol||beans||undefined||haricot Phaseolus vulgaris||Phaseolus vulgaris||Phaseolus vulgaris||Phaseolus vulgaris Ligamiento genético||genetic linkage||undefined||liaison génétique Sede Central By treating the shoot apices of twenty young bean seedlings (Phaseolus vulgaris, 2n = 22) with 0.2% colchicine solution for 20 hours, an autotetraploid sector was obtained from a treated plant. Two seeds were produced from this sector, and the chromosome number of the radicles of these two seeds was cytologically identified as 4n = 44. As compared with the diploid, some degree of gigantism was observed in these two 4n plants. The plants, the leaves, the stomata and the pollen grains were larger than those of the diploid. The 4n plants also had a prolonged reproductive period. About 50% of the pollen grains of the 4n plant showed intense staining with lactophenol-anilin blue, while almost 100% of the pollen grains of the 2n plants showed intense staining. No cells with more than 10 or less than 5 quadrivalents were observed in the pollen mother cells (PMC) in the 4n plants. The average number of quadrivalents per PMC was 7.3 which indicates that an average of 66% of the chromosomes form quadrivalents. The formation of bivalents in the 4n plants occurred in pairs from groups of the four homologues. This explains the high frequency of PMCs with even numbers of bivalents. The frequency of PMCs with univalents was of the same order as that of PMCs with trivalents, suggesting that in the majority of cases, the production of a univalent was due to the formation of a trivalent from the four homologues. 2025-08-21T13:45:39Z 2025-08-21T13:45:39Z 1963-01 Artículo https://repositorio.catie.ac.cr/handle/11554/13732 openAccess es Turrialba; Vol.13, no. 1 8 páginas application/pdf Instituto Interamericano de Ciencias Agrícolas (IICA) |
| spellingShingle | Estudio biológico||biological surveys||undefined||enquête biologique Citología||cytology||Citologia||cytologie Planta||plants||planta||plante Autotetraploidía||autotetraploidy||autotetraploidia||autotétraploïdie Fríjol||beans||undefined||haricot Phaseolus vulgaris||Phaseolus vulgaris||Phaseolus vulgaris||Phaseolus vulgaris Ligamiento genético||genetic linkage||undefined||liaison génétique Sede Central Monge Serrano, F. Moh, C.C. Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris) |
| title | Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris) |
| title_full | Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris) |
| title_fullStr | Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris) |
| title_full_unstemmed | Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris) |
| title_short | Estudio citológico de plantas autotetraploides de frijol (Phaseolus vulgaris) |
| title_sort | estudio citologico de plantas autotetraploides de frijol phaseolus vulgaris |
| topic | Estudio biológico||biological surveys||undefined||enquête biologique Citología||cytology||Citologia||cytologie Planta||plants||planta||plante Autotetraploidía||autotetraploidy||autotetraploidia||autotétraploïdie Fríjol||beans||undefined||haricot Phaseolus vulgaris||Phaseolus vulgaris||Phaseolus vulgaris||Phaseolus vulgaris Ligamiento genético||genetic linkage||undefined||liaison génétique Sede Central |
| url | https://repositorio.catie.ac.cr/handle/11554/13732 |
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