Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao

The study was designed to determine the influence of several factors causing variation in the results obtained with a laboratory test developed by Delgado and Echandi to evaluate resistance of cocoa cultivars to Ceratocystis fimbriata. It was oriented to study the influence of inoculations made: 1)...

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Autores principales: Espinoza, A., Delgado, J.C.
Formato: Artículo
Lenguaje:Español
Publicado: Instituto Interamericano de Cooperación para la Agricultura (IICA) 2025
Materias:
Acceso en línea:https://repositorio.catie.ac.cr/handle/11554/13151
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author Espinoza, A.
Delgado, J.C.
author_browse Delgado, J.C.
Espinoza, A.
author_facet Espinoza, A.
Delgado, J.C.
author_sort Espinoza, A.
collection Repositorio CATIE
description The study was designed to determine the influence of several factors causing variation in the results obtained with a laboratory test developed by Delgado and Echandi to evaluate resistance of cocoa cultivars to Ceratocystis fimbriata. It was oriented to study the influence of inoculations made: 1) at different intervals of time after the branches were cut; 2) on sections from : a) branches of different thickness, b) branches, from different parts of the tree, and c) branches of different flushings; 3) on sections containing different tissues; 4) with inoculum prepared: a) from colonies produced on different substrates, b) with different spore concentrations, c) colonies of different age, and d) colonies obtained by several transfers from one isolated; and 5) seedling with different amounts of inoculum per square centimeter of branch section. A reduced growth of C. fimbriata was found on sections of suckers, even in those susceptible clones. Reduced variations were registered on sections of fan branches, and for this reason, they should be used on the laboratory tests. Also the age of the colonies had considerable influence on the fungus growth, probably due to reduction of spore viability after 30 days
format Artículo
id RepoCATIE13151
institution Centro Agronómico Tropical de Investigación y Enseñanza
language Español
publishDate 2025
publishDateRange 2025
publishDateSort 2025
publisher Instituto Interamericano de Cooperación para la Agricultura (IICA)
publisherStr Instituto Interamericano de Cooperación para la Agricultura (IICA)
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spelling RepoCATIE131512025-07-03T23:22:28Z Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao Espinoza, A. Delgado, J.C. Theobroma cacao||Theobroma cacao||Theobroma cacao||Theobroma cacao Ceratocystis fimbriata||Ceratocystis fimbriata||Ceratocystis fimbriata||Ceratocystis fimbriata Resistencia a la enfermedad||disease resistance||resistência aos agentes patogénicos||résistance aux maladies Inoculación||inoculation||inoculação||inoculation Diagnóstico de laboratorio||laboratory diagnosis||diagnóstico de laboratório||diagnostic de laboratoire Enfermedad fungosa||fungal diseases||doença fúngica||maladie fongique Sede Central The study was designed to determine the influence of several factors causing variation in the results obtained with a laboratory test developed by Delgado and Echandi to evaluate resistance of cocoa cultivars to Ceratocystis fimbriata. It was oriented to study the influence of inoculations made: 1) at different intervals of time after the branches were cut; 2) on sections from : a) branches of different thickness, b) branches, from different parts of the tree, and c) branches of different flushings; 3) on sections containing different tissues; 4) with inoculum prepared: a) from colonies produced on different substrates, b) with different spore concentrations, c) colonies of different age, and d) colonies obtained by several transfers from one isolated; and 5) seedling with different amounts of inoculum per square centimeter of branch section. A reduced growth of C. fimbriata was found on sections of suckers, even in those susceptible clones. Reduced variations were registered on sections of fan branches, and for this reason, they should be used on the laboratory tests. Also the age of the colonies had considerable influence on the fungus growth, probably due to reduction of spore viability after 30 days 2025-07-02T18:20:35Z 2025-07-02T18:20:35Z 1971-01 Artículo https://repositorio.catie.ac.cr/handle/11554/13151 openAccess es Turrialba; Vol.21, no. 1 5 páginas application/pdf Instituto Interamericano de Cooperación para la Agricultura (IICA)
spellingShingle Theobroma cacao||Theobroma cacao||Theobroma cacao||Theobroma cacao
Ceratocystis fimbriata||Ceratocystis fimbriata||Ceratocystis fimbriata||Ceratocystis fimbriata
Resistencia a la enfermedad||disease resistance||resistência aos agentes patogénicos||résistance aux maladies
Inoculación||inoculation||inoculação||inoculation
Diagnóstico de laboratorio||laboratory diagnosis||diagnóstico de laboratório||diagnostic de laboratoire
Enfermedad fungosa||fungal diseases||doença fúngica||maladie fongique
Sede Central
Espinoza, A.
Delgado, J.C.
Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao
title Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao
title_full Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao
title_fullStr Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao
title_full_unstemmed Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao
title_short Factores intrínsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a Ceratocystis fimbriata en cacao
title_sort factores intrinsecos que influyen en la eficacia de la prueba de laboratorio usada para evaluar la resistencia a ceratocystis fimbriata en cacao
topic Theobroma cacao||Theobroma cacao||Theobroma cacao||Theobroma cacao
Ceratocystis fimbriata||Ceratocystis fimbriata||Ceratocystis fimbriata||Ceratocystis fimbriata
Resistencia a la enfermedad||disease resistance||resistência aos agentes patogénicos||résistance aux maladies
Inoculación||inoculation||inoculação||inoculation
Diagnóstico de laboratorio||laboratory diagnosis||diagnóstico de laboratório||diagnostic de laboratoire
Enfermedad fungosa||fungal diseases||doença fúngica||maladie fongique
Sede Central
url https://repositorio.catie.ac.cr/handle/11554/13151
work_keys_str_mv AT espinozaa factoresintrinsecosqueinfluyenenlaeficaciadelapruebadelaboratoriousadaparaevaluarlaresistenciaaceratocystisfimbriataencacao
AT delgadojc factoresintrinsecosqueinfluyenenlaeficaciadelapruebadelaboratoriousadaparaevaluarlaresistenciaaceratocystisfimbriataencacao