Electrophoretic methods for fractionation of humic and fulvic acids
Humic and fulvic acids extracted from Bovizont A of a Brunizem sample with dilute sodium hydroxide solution were separated by isotachophoresis and electrofocusing on a polyacrylamide gel. Ampholine carrier ampholytes ranging in size from 3 to 10 were used as a spacer and to form a natural pH gradien...
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Instituto Interamericano de Cooperación para la Agricultura (IICA)
2025
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| Materias: | |
| Acceso en línea: | https://repositorio.catie.ac.cr/handle/11554/12851 |
| _version_ | 1855488439347052544 |
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| author | Curvetto, N.R. Balmaceda, N.A. Orioli, G.A. |
| author_browse | Balmaceda, N.A. Curvetto, N.R. Orioli, G.A. |
| author_facet | Curvetto, N.R. Balmaceda, N.A. Orioli, G.A. |
| author_sort | Curvetto, N.R. |
| collection | Repositorio CATIE |
| description | Humic and fulvic acids extracted from Bovizont A of a Brunizem sample with dilute sodium hydroxide solution were separated by isotachophoresis and electrofocusing on a polyacrylamide gel. Ampholine carrier ampholytes ranging in size from 3 to 10 were used as a spacer and to form a natural pH gradient. Different pH, pore size and gel geometry were tested during isotachophoresis. The number of subfractions obtained by isotachophoresis on a 3.75% polyacrylamide gel, pH 6.7, was 10 for fulvic acid and 13 for humic acid. When the polarity was reversed, four subfractions separated on the running gel. Electrofocusing in 12-cm-long tubes with 3.75 percent polyacrylamide gel showed a large number of subfractions distributed in pH values between 3.7 and 7.5 for AF and between 4.5 and 7.5 for AH. The position of the bands in the natural pH gradient is considered an indication of their Ka value. |
| format | Artículo |
| id | RepoCATIE12851 |
| institution | Centro Agronómico Tropical de Investigación y Enseñanza |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Instituto Interamericano de Cooperación para la Agricultura (IICA) |
| publisherStr | Instituto Interamericano de Cooperación para la Agricultura (IICA) |
| record_format | dspace |
| spelling | RepoCATIE128512025-05-27T22:10:57Z Electrophoretic methods for fractionation of humic and fulvic acids Métodos electroforéticos para el fraccionamiento de los ácidos húmico y fúlvico Curvetto, N.R. Balmaceda, N.A. Orioli, G.A. Consistencia del suelo||soil consistence||undefined||consistance du sol Ácidos Extracción||extraction||extracção||extraction Suelo sódico||sodic soils||solo sódico||sol sodique Sede Central Humic and fulvic acids extracted from Bovizont A of a Brunizem sample with dilute sodium hydroxide solution were separated by isotachophoresis and electrofocusing on a polyacrylamide gel. Ampholine carrier ampholytes ranging in size from 3 to 10 were used as a spacer and to form a natural pH gradient. Different pH, pore size and gel geometry were tested during isotachophoresis. The number of subfractions obtained by isotachophoresis on a 3.75% polyacrylamide gel, pH 6.7, was 10 for fulvic acid and 13 for humic acid. When the polarity was reversed, four subfractions separated on the running gel. Electrofocusing in 12-cm-long tubes with 3.75 percent polyacrylamide gel showed a large number of subfractions distributed in pH values between 3.7 and 7.5 for AF and between 4.5 and 7.5 for AH. The position of the bands in the natural pH gradient is considered an indication of their Ka value. 2025-05-26T22:26:58Z 2025-05-26T22:26:58Z 1975-10-01 Artículo https://repositorio.catie.ac.cr/handle/11554/12851 openAccess en Turrialba Vol. 25, no. 4 6 application/pdf Instituto Interamericano de Cooperación para la Agricultura (IICA) |
| spellingShingle | Consistencia del suelo||soil consistence||undefined||consistance du sol Ácidos Extracción||extraction||extracção||extraction Suelo sódico||sodic soils||solo sódico||sol sodique Sede Central Curvetto, N.R. Balmaceda, N.A. Orioli, G.A. Electrophoretic methods for fractionation of humic and fulvic acids |
| title | Electrophoretic methods for fractionation of humic and fulvic acids |
| title_full | Electrophoretic methods for fractionation of humic and fulvic acids |
| title_fullStr | Electrophoretic methods for fractionation of humic and fulvic acids |
| title_full_unstemmed | Electrophoretic methods for fractionation of humic and fulvic acids |
| title_short | Electrophoretic methods for fractionation of humic and fulvic acids |
| title_sort | electrophoretic methods for fractionation of humic and fulvic acids |
| topic | Consistencia del suelo||soil consistence||undefined||consistance du sol Ácidos Extracción||extraction||extracção||extraction Suelo sódico||sodic soils||solo sódico||sol sodique Sede Central |
| url | https://repositorio.catie.ac.cr/handle/11554/12851 |
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