Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural

Several methods were tested for detecting the residual inocuhnn of Pseudomonas solanacearum, Race 1, in inceptisols at Turrialba, Costa Rica, where lire bacterium is apparently endemic Planting healthy potato tubers in soil samples in the greenhouse, then using plant wilt as indicator of the pathoge...

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Autores principales: Rodríguez, C.A, González, L.C.
Formato: Artículo
Lenguaje:Español
Publicado: Instituto Interamericano de Cooperación para la Agricultura (IICA) 2022
Materias:
Acceso en línea:https://repositorio.catie.ac.cr/handle/11554/12122
id RepoCATIE12122
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spelling RepoCATIE121222022-11-16T15:40:16Z Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural Determination of the level of primary inoculate of Pseudomonas solanacearum in soil as a natural infestation. Rodríguez, C.A González, L.C. INOCULACION SUELO RALSTONIA SOLANACEARUM MALEZAS PLANTAS INDICADORAS BIDENS PILOSA GALINSOGA COSTA RICA INOCULATION SOIL RALSTONIA SOLANACEARUM WEEDS INDICATOR PLANTS BIDENS PILOSA GALINSOGA COSTA RICA Sede Central Several methods were tested for detecting the residual inocuhnn of Pseudomonas solanacearum, Race 1, in inceptisols at Turrialba, Costa Rica, where lire bacterium is apparently endemic Planting healthy potato tubers in soil samples in the greenhouse, then using plant wilt as indicator of the pathogen's presence, was qualitatively the most efficient method; however, it was not possible to quantify precisely the level of primary inoculum in several soils A selective culture medium containing tetrazolium chloride plus antibiotics allowed detection of P. solanacearum at concentrations near 25 000 cells/gram of dry soil, even though a large number of antagonistic bacteria predominated in many samples, completely inhibiting P. solanaccarum Both methods were used to detect P. solanacearum in samples from soils subjected for nearly four years to three systems of minimum tillage or one of tradi-tional mechanical tillage, populations were consistent), larger in mechanically-tilled soil. This was confirmed by planting potato and tomato directly in the field, where significant contrasts in percentage of bacterial wilt resulted. The decrease of the bacterium in minimum-tilled soils was attributed to an increase in organic matter and thus an increase in antagonistic microorganisms. 2022-11-14T19:09:12Z 2022-11-14T19:09:12Z 1982-10 Artículo https://repositorio.catie.ac.cr/handle/11554/12122 openAccess es Turrialba; Vol. 32, no. 4 12 páginas application/pdf Instituto Interamericano de Cooperación para la Agricultura (IICA)
institution Centro Agronómico Tropical de Investigación y Enseñanza
collection Repositorio CATIE
language Español
topic INOCULACION
SUELO
RALSTONIA SOLANACEARUM
MALEZAS
PLANTAS INDICADORAS
BIDENS PILOSA
GALINSOGA
COSTA RICA
INOCULATION
SOIL
RALSTONIA SOLANACEARUM
WEEDS
INDICATOR PLANTS
BIDENS PILOSA
GALINSOGA
COSTA RICA
Sede Central
spellingShingle INOCULACION
SUELO
RALSTONIA SOLANACEARUM
MALEZAS
PLANTAS INDICADORAS
BIDENS PILOSA
GALINSOGA
COSTA RICA
INOCULATION
SOIL
RALSTONIA SOLANACEARUM
WEEDS
INDICATOR PLANTS
BIDENS PILOSA
GALINSOGA
COSTA RICA
Sede Central
Rodríguez, C.A
González, L.C.
Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural
description Several methods were tested for detecting the residual inocuhnn of Pseudomonas solanacearum, Race 1, in inceptisols at Turrialba, Costa Rica, where lire bacterium is apparently endemic Planting healthy potato tubers in soil samples in the greenhouse, then using plant wilt as indicator of the pathogen's presence, was qualitatively the most efficient method; however, it was not possible to quantify precisely the level of primary inoculum in several soils A selective culture medium containing tetrazolium chloride plus antibiotics allowed detection of P. solanacearum at concentrations near 25 000 cells/gram of dry soil, even though a large number of antagonistic bacteria predominated in many samples, completely inhibiting P. solanaccarum Both methods were used to detect P. solanacearum in samples from soils subjected for nearly four years to three systems of minimum tillage or one of tradi-tional mechanical tillage, populations were consistent), larger in mechanically-tilled soil. This was confirmed by planting potato and tomato directly in the field, where significant contrasts in percentage of bacterial wilt resulted. The decrease of the bacterium in minimum-tilled soils was attributed to an increase in organic matter and thus an increase in antagonistic microorganisms.
format Artículo
author Rodríguez, C.A
González, L.C.
author_facet Rodríguez, C.A
González, L.C.
author_sort Rodríguez, C.A
title Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural
title_short Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural
title_full Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural
title_fullStr Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural
title_full_unstemmed Determinación del nivel de inóculo primario de Pseudomonas solanacearum en suelos con infestación natural
title_sort determinación del nivel de inóculo primario de pseudomonas solanacearum en suelos con infestación natural
publisher Instituto Interamericano de Cooperación para la Agricultura (IICA)
publishDate 2022
url https://repositorio.catie.ac.cr/handle/11554/12122
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AT rodriguezca determinationofthelevelofprimaryinoculateofpseudomonassolanacearuminsoilasanaturalinfestation
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