Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación.

The comparative efficiency of the centrifigation-flotation (CF) and modified Baermann Funnel (MBF) techniques, as well as some modifications, for the extraction of Meloidogyne incognita larvae from three soil types commonly found in Costa Rica (Ustult, Ustropept and Distropept) was evaluated. Modifi...

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Autores principales: Alvarado S, M, López Ch, R.
Formato: Artículo
Lenguaje:Español
Publicado: Instituto Interamericano de Cooperación para la Agricultura (IICA) 2022
Materias:
Acceso en línea:https://repositorio.catie.ac.cr/handle/11554/12065
id RepoCATIE12065
record_format dspace
spelling RepoCATIE120652022-10-11T16:07:01Z Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación. Extraction of Meloidogyne incognita larvae from three tropical soils through modification of the modified Baermann Funnel and the centrifugation-flotation techniques. Alvarado S, M López Ch, R. MELOIDOGYNE INCOGNITA CENTRIFUGACION FLOTACION MELOIDOGYNE INCOGNITA CENTRIFUGING FLOTATION Sede Central The comparative efficiency of the centrifigation-flotation (CF) and modified Baermann Funnel (MBF) techniques, as well as some modifications, for the extraction of Meloidogyne incognita larvae from three soil types commonly found in Costa Rica (Ustult, Ustropept and Distropept) was evaluated. Modifications evaluated with both techniques were sample volume (50, 100 or 150 cc), number of sample washings (1, 2 or 3), soil suspension time before each washing (20, 40 or 60 sec) and sieve arrangement (A: one 100 and two 325 mesh sieves; B: one 50 and two 325 mesh sieves, and C: one 100 and 3.25 mesh sieves). Centrifugation time (3, 4 or .5 min. at 3000 rpm) and specific gravity of the sugar solution (1.12, 1.15 or 1.18) were also evaluated with the CF technique. Significantly more larvae were extracted with 100 cc soil samples in the Ustropept and Ustult soils with the MBF method. Significantly higher numbers of larvae were also recovered from the Ustropept soil with three sample washings whereas higher numbers were obtained from Ustult and Distropept soils with 20 and 60 sec of soil suspension before each washing, respectively, with the CF technique; one sample washing also yielded higher numbers of larvae in the Distropept soil The B sieve arrangement and the 1.12 specific gravity sugar solution recovered higher larvae densities from the Ustropept and Distropept soils, respectively. Significantly more larvae were recovered from the three soil types with the CF than with the MBF technique. 2022-10-10T20:01:07Z 2022-10-10T20:01:07Z 1982-01 Artículo https://repositorio.catie.ac.cr/handle/11554/12065 openAccess es Turrialba; Vol. 32, no. 1 6 páginas application/pdf Instituto Interamericano de Cooperación para la Agricultura (IICA)
institution Centro Agronómico Tropical de Investigación y Enseñanza
collection Repositorio CATIE
language Español
topic MELOIDOGYNE INCOGNITA
CENTRIFUGACION
FLOTACION
MELOIDOGYNE INCOGNITA
CENTRIFUGING
FLOTATION
Sede Central
spellingShingle MELOIDOGYNE INCOGNITA
CENTRIFUGACION
FLOTACION
MELOIDOGYNE INCOGNITA
CENTRIFUGING
FLOTATION
Sede Central
Alvarado S, M
López Ch, R.
Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación.
description The comparative efficiency of the centrifigation-flotation (CF) and modified Baermann Funnel (MBF) techniques, as well as some modifications, for the extraction of Meloidogyne incognita larvae from three soil types commonly found in Costa Rica (Ustult, Ustropept and Distropept) was evaluated. Modifications evaluated with both techniques were sample volume (50, 100 or 150 cc), number of sample washings (1, 2 or 3), soil suspension time before each washing (20, 40 or 60 sec) and sieve arrangement (A: one 100 and two 325 mesh sieves; B: one 50 and two 325 mesh sieves, and C: one 100 and 3.25 mesh sieves). Centrifugation time (3, 4 or .5 min. at 3000 rpm) and specific gravity of the sugar solution (1.12, 1.15 or 1.18) were also evaluated with the CF technique. Significantly more larvae were extracted with 100 cc soil samples in the Ustropept and Ustult soils with the MBF method. Significantly higher numbers of larvae were also recovered from the Ustropept soil with three sample washings whereas higher numbers were obtained from Ustult and Distropept soils with 20 and 60 sec of soil suspension before each washing, respectively, with the CF technique; one sample washing also yielded higher numbers of larvae in the Distropept soil The B sieve arrangement and the 1.12 specific gravity sugar solution recovered higher larvae densities from the Ustropept and Distropept soils, respectively. Significantly more larvae were recovered from the three soil types with the CF than with the MBF technique.
format Artículo
author Alvarado S, M
López Ch, R.
author_facet Alvarado S, M
López Ch, R.
author_sort Alvarado S, M
title Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación.
title_short Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación.
title_full Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación.
title_fullStr Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación.
title_full_unstemmed Recuperación de larvas de Meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del Embudo de Baermann modificado y centrifugación-flotación.
title_sort recuperación de larvas de meloidogyne incognita de tres suelos tropicales por modificaciones de las técnicas del embudo de baermann modificado y centrifugación-flotación.
publisher Instituto Interamericano de Cooperación para la Agricultura (IICA)
publishDate 2022
url https://repositorio.catie.ac.cr/handle/11554/12065
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