Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder
Under ideal conditions, Escherichia coli cells divide after adding a fixed cell size, a strategy known as the adder. This concept applies to various microbes and is often explained as the division that occurs after a certain number of stages, associated with the accumulation of precursor proteins...
| Autores principales: | , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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Nature Research
2025
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| Acceso en línea: | https://www.nature.com/articles/s41540-024-00383-z http://hdl.handle.net/20.500.12324/41190 https://doi.org/10.1038/s41540-024-00383-z |
| _version_ | 1855494513886232576 |
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| author | Nieto, César Vargas García, César Augusto Pedraza, Juan Manuel Singh, Abhyudai |
| author_browse | Nieto, César Pedraza, Juan Manuel Singh, Abhyudai Vargas García, César Augusto |
| author_facet | Nieto, César Vargas García, César Augusto Pedraza, Juan Manuel Singh, Abhyudai |
| author_sort | Nieto, César |
| collection | Repositorio AGROSAVIA |
| description | Under ideal conditions, Escherichia coli cells divide after adding a fixed cell size, a strategy known as
the adder. This concept applies to various microbes and is often explained as the division that occurs
after a certain number of stages, associated with the accumulation of precursor proteins at a rate
proportional to cell size. However, under poor media conditions, E. coli cells exhibit a different size
regulation. They are smaller and follow a sizer-like division strategy where the added size is inversely
proportional to the size at birth. We explore three potential causes for this deviation: degradation of the
precursor protein and two models where the propensity for accumulation depends on the cell size: a
nonlinear accumulation rate, and accumulation starting at a threshold size termed the commitment
size. These models fit the mean trends but predict different distributions given the birth size. To
quantify the precision of the models to explain the data, we used the Akaike information criterion and
compared them to open datasets of slow-growing E. coli cells in different media. We found that none of
the models alone can consistently explain the data. However, the degradation model better explains
the division strategy when cells are larger, whereas size-related models (power-law and commitment
size) account for smaller cells. Our methodology proposes a data-based method in which different
mechanisms can be tested systematically |
| format | Artículo |
| id | RepoAGROSAVIA41190 |
| institution | Corporación Colombiana de Investigación Agropecuaria |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Nature Research |
| publisherStr | Nature Research |
| record_format | dspace |
| spelling | RepoAGROSAVIA411902025-09-06T03:00:23Z Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder Nieto, César Vargas García, César Augusto Pedraza, Juan Manuel Singh, Abhyudai Investigación agropecuaria - A50 Escherichia coli Biología molecular Proteínas Transversal http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_13621 Under ideal conditions, Escherichia coli cells divide after adding a fixed cell size, a strategy known as the adder. This concept applies to various microbes and is often explained as the division that occurs after a certain number of stages, associated with the accumulation of precursor proteins at a rate proportional to cell size. However, under poor media conditions, E. coli cells exhibit a different size regulation. They are smaller and follow a sizer-like division strategy where the added size is inversely proportional to the size at birth. We explore three potential causes for this deviation: degradation of the precursor protein and two models where the propensity for accumulation depends on the cell size: a nonlinear accumulation rate, and accumulation starting at a threshold size termed the commitment size. These models fit the mean trends but predict different distributions given the birth size. To quantify the precision of the models to explain the data, we used the Akaike information criterion and compared them to open datasets of slow-growing E. coli cells in different media. We found that none of the models alone can consistently explain the data. However, the degradation model better explains the division strategy when cells are larger, whereas size-related models (power-law and commitment size) account for smaller cells. Our methodology proposes a data-based method in which different mechanisms can be tested systematically U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (R35GM148351) 2025-09-05T14:32:44Z 2025-09-05T14:32:44Z 2024-05-29 2024 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.nature.com/articles/s41540-024-00383-z 2056-7189 http://hdl.handle.net/20.500.12324/41190 https://doi.org/10.1038/s41540-024-00383-z reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng npj Systems Biology and Applications 10 61 Vargas-Garcia, C. A., Soltani, M. & Singh, A. Conditions for cell size homeostasis: a stochastic hybrid system approach. IEEE Life Sci. Lett. 2, 47–50 (2016). Taheri-Araghi, S. et al. 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| spellingShingle | Investigación agropecuaria - A50 Escherichia coli Biología molecular Proteínas Transversal http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_13621 Nieto, César Vargas García, César Augusto Pedraza, Juan Manuel Singh, Abhyudai Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder |
| title | Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder |
| title_full | Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder |
| title_fullStr | Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder |
| title_full_unstemmed | Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder |
| title_short | Mechanisms of cell size regulation in slow-growing Escherichia coli cells: discriminating models beyond the adder |
| title_sort | mechanisms of cell size regulation in slow growing escherichia coli cells discriminating models beyond the adder |
| topic | Investigación agropecuaria - A50 Escherichia coli Biología molecular Proteínas Transversal http://aims.fao.org/aos/agrovoc/c_33700 http://aims.fao.org/aos/agrovoc/c_4891 http://aims.fao.org/aos/agrovoc/c_13621 |
| url | https://www.nature.com/articles/s41540-024-00383-z http://hdl.handle.net/20.500.12324/41190 https://doi.org/10.1038/s41540-024-00383-z |
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