Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits

In Colombia, Venezuela, Ecuador, Brazil and Honduras, the tomato borer, Neoleucinodes elegantalis, is the most important fruit-related plague of the Solanaceae family. A suitable molecular characterization using a DNA barcoding system is necessary to clarify different issues inside the taxonomy of...

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Main Authors:	Gallego Sánchez, Gerardo J., Zapata, Patricia, Castañeda, Oscar, Suárez Baron, Harold, Díaz Montilla, Ana Elizabeth, Vásquez, Wilson, Tohme, Joseph M.
Format:	article
Language:	Inglés
Published:	CGIAR research centers 2025
Subjects:	Genética vegetal y fitomejoramiento - F30 ADN Morfología Gen Transversal http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_49903 http://aims.fao.org/aos/agrovoc/c_3214
Online Access:	https://cgspace.cgiar.org/items/1e1cfb94-b91c-4051-8bf3-69c3bf4b4740 http://hdl.handle.net/20.500.12324/41000 https://hdl.handle.net/10568/67052

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spelling	RepoAGROSAVIA410002025-06-17T03:02:17Z Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits Gallego Sánchez, Gerardo J. Zapata, Patricia Castañeda, Oscar Suárez Baron, Harold Díaz Montilla, Ana Elizabeth Vásquez, Wilson Tohme, Joseph M. Genética vegetal y fitomejoramiento - F30 ADN Morfología Gen Transversal http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_49903 http://aims.fao.org/aos/agrovoc/c_3214 In Colombia, Venezuela, Ecuador, Brazil and Honduras, the tomato borer, Neoleucinodes elegantalis, is the most important fruit-related plague of the Solanaceae family. A suitable molecular characterization using a DNA barcoding system is necessary to clarify different issues inside the taxonomy of Neoleucinodes genus. Additionally, other DNA sequences used for molecular identification and phylogenetics studies, can be implemented to obtain a better understanding of the genetic variability across different animal groups and allows to acquire a enhanced description of the populations genetic variation. The main objectives of this study are: 1. Evaluate the performance of DNA barcoding sequences (COI gen and 18S rDNA gene), in the genetic characterization of populations of N. elegantalis, collected in different wild and cultivated solanaceous plants in Colombia and Ecuador. 2. Determination of possible haplotypes related with each population belonging to this species. 3.Identification of geographical patterns associated with the distribution of this insect. 2025-06-16T20:50:41Z 2025-06-16T20:50:41Z 2015 2015 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://cgspace.cgiar.org/items/1e1cfb94-b91c-4051-8bf3-69c3bf4b4740 http://hdl.handle.net/20.500.12324/41000 https://hdl.handle.net/10568/67052 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng CGIAR research centers 1 1 1 1 DIAZ, A. E.; SOLIS, M. A. 2007. A New species and species distribution records of Neoleucinodes (Lepidoptera: Crambidae:Spilomelinae) from Colombia feeding on Solanum sp. Proceedings of the Entomological Society of Washington. 109 (4):897–908. FOLMER, O., M. BLACK, W. HOEH, R. LUTZ, AND R. VRIJENHOEK.1994. DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates.Mol. Mar. Biol. Biotechnol. 3: 294-297. GILBERTSON, R. L.; ROJAS, M.R.; RUSSELL, D.; MAXWELL, D. P. 1991. The use of the asymmetric polymerase chain reaction and DNA sequencing to determinate genetic variability among isolates of bean golden mosaic geminivirus in the Dominican Republic. J. Gen. Virol. 72: 2843-2848. KATTAN, G. H.; FRANCO, P.; ROJAS, V.; MORALES, G. 2004. Biological diversification in a complex región: a spatial analysis of faunistic diversity and biogeography of the Andes of Colombia. Journal of Biogeography, 31, 18291839. Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf C.I La Selva Colombia CGIAR research centers CGIAR research centers; (2015): CGIAR research centers ;p. 1 - 1.
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Genética vegetal y fitomejoramiento - F30 ADN Morfología Gen Transversal http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_49903 http://aims.fao.org/aos/agrovoc/c_3214
spellingShingle	Genética vegetal y fitomejoramiento - F30 ADN Morfología Gen Transversal http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_49903 http://aims.fao.org/aos/agrovoc/c_3214 Gallego Sánchez, Gerardo J. Zapata, Patricia Castañeda, Oscar Suárez Baron, Harold Díaz Montilla, Ana Elizabeth Vásquez, Wilson Tohme, Joseph M. Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits
description	In Colombia, Venezuela, Ecuador, Brazil and Honduras, the tomato borer, Neoleucinodes elegantalis, is the most important fruit-related plague of the Solanaceae family. A suitable molecular characterization using a DNA barcoding system is necessary to clarify different issues inside the taxonomy of Neoleucinodes genus. Additionally, other DNA sequences used for molecular identification and phylogenetics studies, can be implemented to obtain a better understanding of the genetic variability across different animal groups and allows to acquire a enhanced description of the populations genetic variation. The main objectives of this study are: 1. Evaluate the performance of DNA barcoding sequences (COI gen and 18S rDNA gene), in the genetic characterization of populations of N. elegantalis, collected in different wild and cultivated solanaceous plants in Colombia and Ecuador. 2. Determination of possible haplotypes related with each population belonging to this species. 3.Identification of geographical patterns associated with the distribution of this insect.
format	article
author	Gallego Sánchez, Gerardo J. Zapata, Patricia Castañeda, Oscar Suárez Baron, Harold Díaz Montilla, Ana Elizabeth Vásquez, Wilson Tohme, Joseph M.
author_facet	Gallego Sánchez, Gerardo J. Zapata, Patricia Castañeda, Oscar Suárez Baron, Harold Díaz Montilla, Ana Elizabeth Vásquez, Wilson Tohme, Joseph M.
author_sort	Gallego Sánchez, Gerardo J.
title	Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits
title_short	Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits
title_full	Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits
title_fullStr	Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits
title_full_unstemmed	Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits
title_sort	use of dna sequences for identification of possible biotypes of the fruit borer neoleucinodes elegantalis (lepidoptera: crambidae), an important pest of andean solanaceous fruits
publisher	CGIAR research centers
publishDate	2025
url	https://cgspace.cgiar.org/items/1e1cfb94-b91c-4051-8bf3-69c3bf4b4740 http://hdl.handle.net/20.500.12324/41000 https://hdl.handle.net/10568/67052
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Use of DNA sequences for identification of possible biotypes of the fruit borer Neoleucinodes elegantalis (Lepidoptera: Crambidae), an important pest of Andean solanaceous fruits

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