The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster

Specific mate communication and recognition underlies reproduction and hence speciation. Mate communication evolves during adaptation to ecological niches and makes use of social signals and habitat cues. Our study provides new insights in Drosophila melanogaster premating olfactory communication...

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Main Authors:	Borrero Echeverry, Felipe, Marit, Solum, Trona, Federica, Wallin, Erika A., Bengtsson, Marie, Witzgall, Peter, Lebreton, Sebastien
Format:	article
Language:	Inglés
Published:	Cold Sprimg Harbor Laboratory - CSH 2025
Subjects:	Genética y mejoramiento animal - L10 Avicultura Reproducción animal Mejoramiento genético Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_742 http://aims.fao.org/aos/agrovoc/c_5d5904a8 http://aims.fao.org/aos/agrovoc/c_11119
Online Access:	https://www.biorxiv.org/content/10.1101/2021.01.06.425638v2 http://hdl.handle.net/20.500.12324/40998

id	RepoAGROSAVIA40998
record_format	dspace
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Genética y mejoramiento animal - L10 Avicultura Reproducción animal Mejoramiento genético Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_742 http://aims.fao.org/aos/agrovoc/c_5d5904a8 http://aims.fao.org/aos/agrovoc/c_11119
spellingShingle	Genética y mejoramiento animal - L10 Avicultura Reproducción animal Mejoramiento genético Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_742 http://aims.fao.org/aos/agrovoc/c_5d5904a8 http://aims.fao.org/aos/agrovoc/c_11119 Borrero Echeverry, Felipe Marit, Solum Trona, Federica Wallin, Erika A. Bengtsson, Marie Witzgall, Peter Lebreton, Sebastien The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster
description	Specific mate communication and recognition underlies reproduction and hence speciation. Mate communication evolves during adaptation to ecological niches and makes use of social signals and habitat cues. Our study provides new insights in Drosophila melanogaster premating olfactory communication, showing that female pheromone (Z)-4-undecenal (Z4-11Al) and male pheromone cVA interact with food odour in a sex-specific manner. Furthermore, Z4-11Al, which mediates upwind flight attraction in both sexes, also elicits courtship in experienced males. Twin variants of the olfactory receptor Or69a are co-expressed in the same olfactory sensory neurons, and feed into the same glomerulus in the antennal lobe. Z4-11Al is perceived via Or69aB, while the food odorant (R)-linalool is a main ligand for the other variant, Or69aA. That Z4-11Al mediates courtship in experienced males, not (R)- linalool, is probably due to courtship learning. Behavioural discrimination is reflected by calcium imaging of the antennal lobe, showing distinct glomerular activation patterns by these two compounds. Male sex pheromone cVA is known to affect male and female courtship at close range, but does not elicit upwind flight attraction as a single compound, in to contrast to Z4-11Al. A blend of cVA and the food odour vinegar attracted females, while a blend of female pheromone Z4-11Al and vinegar attracted males instead. Sex-specific upwind flight attraction to blends of food volatiles and male and female pheromone, respectively, adds a new element to Drosophila olfactory premating communication and is an unambiguous paradigm for identifying the behaviourally active components, towards a more complete concept of food-pheromone odour objects.
format	article
author	Borrero Echeverry, Felipe Marit, Solum Trona, Federica Wallin, Erika A. Bengtsson, Marie Witzgall, Peter Lebreton, Sebastien
author_facet	Borrero Echeverry, Felipe Marit, Solum Trona, Federica Wallin, Erika A. Bengtsson, Marie Witzgall, Peter Lebreton, Sebastien
author_sort	Borrero Echeverry, Felipe
title	The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster
title_short	The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster
title_full	The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster
title_fullStr	The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster
title_full_unstemmed	The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster
title_sort	female pheromone (z)-4-undecenal mediates flight attraction and courtship in drosophila melanogaster
publisher	Cold Sprimg Harbor Laboratory - CSH
publishDate	2025
url	https://www.biorxiv.org/content/10.1101/2021.01.06.425638v2 http://hdl.handle.net/20.500.12324/40998
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spelling	RepoAGROSAVIA409982025-06-17T03:00:25Z The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster Borrero Echeverry, Felipe Marit, Solum Trona, Federica Wallin, Erika A. Bengtsson, Marie Witzgall, Peter Lebreton, Sebastien Genética y mejoramiento animal - L10 Avicultura Reproducción animal Mejoramiento genético Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_742 http://aims.fao.org/aos/agrovoc/c_5d5904a8 http://aims.fao.org/aos/agrovoc/c_11119 Specific mate communication and recognition underlies reproduction and hence speciation. Mate communication evolves during adaptation to ecological niches and makes use of social signals and habitat cues. Our study provides new insights in Drosophila melanogaster premating olfactory communication, showing that female pheromone (Z)-4-undecenal (Z4-11Al) and male pheromone cVA interact with food odour in a sex-specific manner. Furthermore, Z4-11Al, which mediates upwind flight attraction in both sexes, also elicits courtship in experienced males. Twin variants of the olfactory receptor Or69a are co-expressed in the same olfactory sensory neurons, and feed into the same glomerulus in the antennal lobe. Z4-11Al is perceived via Or69aB, while the food odorant (R)-linalool is a main ligand for the other variant, Or69aA. That Z4-11Al mediates courtship in experienced males, not (R)- linalool, is probably due to courtship learning. Behavioural discrimination is reflected by calcium imaging of the antennal lobe, showing distinct glomerular activation patterns by these two compounds. Male sex pheromone cVA is known to affect male and female courtship at close range, but does not elicit upwind flight attraction as a single compound, in to contrast to Z4-11Al. A blend of cVA and the food odour vinegar attracted females, while a blend of female pheromone Z4-11Al and vinegar attracted males instead. Sex-specific upwind flight attraction to blends of food volatiles and male and female pheromone, respectively, adds a new element to Drosophila olfactory premating communication and is an unambiguous paradigm for identifying the behaviourally active components, towards a more complete concept of food-pheromone odour objects. Colombian Corporation for Agricultural Research - Corpoica Colombian Administrative Department of Science, Technology, and Innovation - Colciencias Avicultura 2025-06-16T20:30:33Z 2025-06-16T20:30:33Z 2021-01 2021 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.biorxiv.org/content/10.1101/2021.01.06.425638v2 http://hdl.handle.net/20.500.12324/40998 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng BioRxiv 1 1 1 22 Alves, H., Rouault, J. D., Kondoh, Y., Nakano, Y., Yamamoto, D., Kim, Y. K., & Jallon, J. 415 M. (2010). Evolution of cuticular hydrocarbons of Hawaiian Drosophilidae. Behavior 416 Genetics, 40(5), 694-705. (doi:10.1007/s10519-010-9364-y) Ando, Y., Yoshimizu, T., & Matsuo, T. (2020). Food availability reverses the effect of 418 hunger state on copulation rate in Drosophila prolongata females. Animal 419 Behaviour, 166, 51-59. (doi:10.1016/j.anbehav.2020.06.003) Auer, T. O., & Benton, R. (2016). Sexual circuitry in Drosophila. Current Opinion in 421 Neurobiology 38:18-26. (doi:10.1016/j.conb.2016.01.004) Auer TO, Khallaf MA, Silbering AF, Zappia G, Ellis K, Alvarez-Ocana R, Arguello JR, 423 Hansson BS, Jefferis GS, Caron SJ, Knaden M, Benton R. 2020. Olfactory receptor 424 and circuit evolution promote host specialization. Nature 579:402-408. 425 (doi:10.1038/s41586-020-2073-7) Bartelt, R.J., Schaner, A.M. & Jackson, L.L. 1985 cis-Vaccenyl acetate as an aggregation 427 pheromone in Drosophila melanogaster. J. Chem. Ecol. 11, 1747-1756. 428 (doi:10.1007/BF01012124) Bates AS, Schlegel P, Roberts RJV, Drummond N, Tamimi IFM, Turnbull R, Zhao X, 430 Marin EC, Popovici PD, Dhawan S, Jamasb A, Javier A, Capdevila LS, Li F, Rubin 431 GM, Waddell S, Bock DD, Costa M, Jefferis GSXE. 2020. Complete connectomic 432 reconstruction of olfactory projection neurons in the fly brain. Current Biology 30, 433 3183-3199. (doi.org/10.1016/j.cub.2020.06.042) Becher PG, Bengtsson M, Hansson BS, Witzgall P. 2010. Flying the fly: long-range flight 435 behavior of Drosophila melanogaster to attractive odors. J chem Ecol 36:599-607 436 (doi: 10.1007/s10886-010-9794-2) Becher, P.G., Flick, G., Rozpędowska, E., Schmidt, A., Hagman, A., Lebreton, S., 438 Larsson, M.C., Hansson, B.S., Piškur, J., Witzgall, P., et al. 2012 Yeast, not fruit 439 volatiles mediate Drosophila melanogaster attraction, oviposition and development. 440 Funct. Ecol. 26, 822-828. (doi:10.1111/j.1365-2435.2012.02006.x). Billeter, J. C., Rideout, E. J., Dornan, A. J, Goodwin SF. Control of male sexual behavior 442 in Drosophila by the sex determination pathway. Curr. Biol. 16, R766–R776 (2006). Billeter JC, Atallah J, Krupp JJ, Millar JG, Levine JD. 2009. Specialized cells tag sexual 444 and species identity in Drosophila melanogaster. Nature 461:987-U250 445 (doi:10.1038/nature08495) Billeter, J. C., & Wolfner, M. F. (2018). Chemical cues that guide female reproduction in 447 Drosophila melanogaster. Journal of chemical Ecology 44:750-769. 448 (doi:10.1007/s10886-018-0947-z) Buser, C.C., Newcomb, R.D., Gaskett, A.C. & Goddard, M.R. 2014 Niche construction 450 initiates the evolution of mutualistic interactions. Ecol. Lett. 17, 1257-1264. 451 (doi:10.1111/ele.12331). Cachero, S., Ostrovsky, A. D., Yu, J. Y., Dickson, B. J. & Jefferis, G. S. X. E. Sexual 453 dimorphism in the fly brain. Curr. Biol. 20, 1589–1601 (2010). Callejón, R.M., Tesfaye, W., Torija, M.J., Mas, A., Troncoso, A.M. and Morales, M.L., 455 2009. Volatile compounds in red wine vinegars obtained by submerged and surface 456 acetification in different woods. Food Chemistry, 113(4), 1252-1259. 457 (doi:10.1016/j.foodchem.2008.08.027) Cazale-Debat, L., Houot, B., Farine, J. P., Everaerts, C., & Ferveur, J. F. (2019). Flying 459 Drosophila show sex-specific attraction to fly-labelled food. Scientific reports, 9(1), 460 1-13. (doi:10.1038/s41598-019-51351-1) Chinnici, F., Duran Guerrero, E., Sonni, F., Natali, N., Natera Marin, R. and Riponi, C., 462 2009. Gas Chromatography− Mass Spectrometry (GC− MS) characterization of 463 volatile compounds in quality vinegars with protected European geographical 464 indication. Journal of agricultural and food chemistry, 57(11), 4784-4792. 465 (doi:10.1021/jf804005w) Christiaens, J.F., Franco, L.M., Cools, T.L., De Meester, L., Michiels, J., Wenseleers, T., 467 Hassan, B.A., Yaksi, E. & Verstrepen, K.J. 2014 The fungal aroma gene ATF1 468 promotes dispersal of yeast cells through insect vectors. Cell Reports 9, 425-432. 469 (doi:10.1016/j.celrep.2014.09.009). Clowney, E.J., Iguchi, S., Bussell, J.J., Scheer, E. & Ruta, V. 2015 Multimodal 471 Chemosensory Circuits Controlling Male Courtship in Drosophila. Neuron 87, 1036- 472 1049. (doi:10.1016/j.neuron.2015.07.025). Conceicao IC, Aguade M. 2008. High incidence of interchromosomal transpositions in 474 the evolutionary history of a subset of Or genes in Drosophila. J Molec Evol. 475 66:325-32. (doi:10.1007/s00239-008-9071-y) Couto A, Alenius M, Dickson BJ. 2005. Molecular, anatomical, and functional 477 organization of the Drosophila olfactory system. Current Biology 15:1535-1547 478 (doi:10.1016/j.cub.2005.07.034) Das S, Trona F, Khallaf MA, Schuh E, Knaden M, Hansson BS, Sachse S. 2017. Electrical 480 synapses mediate synergism between pheromone and food odors in Drosophila 481 melanogaster. Proc Ntl Acad Sci USA 114:E9962-E9971. 482 (doi:10.1073/pnas.1712706114) Davis, J. S., Pearcy, M. A., Yew, J. Y., & Moyle, L. C. (2020). A shift to shorter cuticular 484 hydrocarbons causes sexual isolation in the Drosophila americana species group. 485 bioRxiv (doi:10.1101/2020.09.07.286294) De Oliveira, C. C., Manfrin, M. H., de M Sene, F., Jackson, L. L., & Etges, W. J. 2011. 487 Variations on a theme: diversification of cuticular hydrocarbons in a clade of 488 cactophilic Drosophila. BMC Evolutionary Biology 11:179. (doi:10.1186/1471-2148- 489 11-179) Dekker, T., Ibba, I., Siju, K. P., Stensmyr, M. C., & Hansson, B. S. (2006). Olfactory 491 shifts parallel superspecialism for toxic fruit in Drosophila melanogaster sibling, D. 492 sechellia. Current Biology 16:101-109. (doi:10.1016/j.cub.2005.11.075) Depetris-Chauvin, A., Galagovsky, D. & Grosjean, Y. 2015 Chemicals and 494 chemoreceptors: ecologically relevant signals driving behavior in Drosophila. Front 495 Ecol Evol 3. (doi:10.3389/fevo.2015.00041). Ejima A, Smith BPC, Lucas C, Van Naters WV, Miller CJ, Carlson JR, Levine JD, Griffith 497 LC. 2007. Generalization of courtship learning in Drosophila is mediated by cis498 vaccenyl acetate. Curr Biol 17:599-605. (doi:10.1016/j.cub.2007.01.053) El-Sayed, A. M. (2020). The pherobase: database of pheromones and semiochemicals. 500 www.phero base.com Endo K, Tsuchimoto Y, Kazama H. 2020. Synthesis of conserved odor object 502 representations in a random, divergent-convergent network. Neuron 108, 367– 503 381. (doi:10.1016/j.neuron.2020.07.029) Goldman, A. L., van Naters, W. V. D. G., Lessing, D., Warr, C. G., & Carlson, J. R. 505 (2005). Coexpression of two functional odor receptors in one neuron. Neuron 506 45:661-666. (doi:10.1016/j.neuron.2005.01.025) Gorter, J.A., Jagadeesh, S., Gahr, C., Boonekamp, J.J., Levine, J.D. and Billeter, J.C., 508 2016. The nutritional and hedonic value of food modulate sexual receptivity in 509 Drosophila melanogaster females. Scientific reports, 6, 19441 510 (doi:10.1038/srep19441) Grabe, V., Strutz, A., Baschwitz, A., Hansson, B.S. and Sachse, S., 2015. Digital in vivo 512 3D atlas of the antennal lobe of Drosophila melanogaster. Journal of Comparative 513 Neurology, 523(3), pp.530-544 (doi:10.1002/cne.23697). Grabe, V., Baschwitz, A., Dweck, H.K., Lavista-Llanos, S., Hansson, B.S. and Sachse, 515 S., 2016. Elucidating the neuronal architecture of olfactory glomeruli in the 516 Drosophila antennal lobe. Cell Reports, 16(12), pp.3401-3413. 517 (doi:10.1016/j.celrep.2016.08.063) Grangeteau, C., Yahou, F., Everaerts, C., Dupont, S., Farine, J.P., Beney, L. and 519 Ferveur, J.F. 2018. Yeast quality in juvenile diet affects Drosophila melanogaster 520 adult life traits. Scientific Reports 8, 1-9. (doi:10.1038/s41598-018-31561-9) Grosjean Y, Rytz R, Farine JP, Abuin L, Cortot J, Jefferis GSXE, Benton R. 2011. An 522 olfactory receptor for food-derived odours promotes male courtship in Drosophila. 523 Nature 478:236-U123. (doi: 10.1038/nature10428) Howard, R.W., Jackson, L.L., Banse, H. and Blows, M.W., 2003. Cuticular hydrocarbons 525 of Drosophila birchii and D. serrata: identification and role in mate choice in D. 526 serrata. Journal of Chemical Ecology 29(4):961-976. 527 (doi:10.1023/A:1022992002239) Keleman, K., Vrontou, E., Krüttner, S., Jai, Y. Y., Kurtovic-Kozaric, A., & Dickson, B. J. 529 (2012). Dopamine neurons modulate pheromone responses in Drosophila courtship 530 learning. Nature 489:145-149. (doi:10.1038/nature11345) Khallaf MA, Auer TO, Grabe V, Depetris-Chauvin A, Ammagarahalli B, Zhang D-D, 532 Lavista-Llanos S, Kaftan F, Weißflog J, Matzkin LM, Rollmann SM, Löfstedt C, 533 Svatos A, Dweck HKM, Sachse S, Benton R, Hansson BS, Knaden M. 2020a. Mate 534 discrimination among subspecies through a conserved olfactory pathway. Sci. Adv. 535 6: eaba5279. (doi:10.1126/sciadv.aba5279) Khallaf, M.A., Cui, R., Weißflog, J., Svatoš, A., Dweck, H., Valenzano, D.R., Hansson, 537 B.S. and Knaden, M., 2020b. Large-scale characterization of sex pheromone 538 communication systems in Drosophila. bioRxiv, September 22 539 (doi:10.1101/2020.09.21.305854) Kimura, K., Hachiya, T., Koganezawa, M., Tazawa, T., Yamamoto, D. 2008. Fruitless 541 and Doublesex coordinate to generate male-specific neurons that can courtship. 542 Neuron 59, 759-769. (doi:10.1038/nrn3567) Kohl, J., Ostrovsky, Aaron D., Frechter, S. & Jefferis, Gregory S. 2013 A Bidirectional 544 Circuit Switch Reroutes Pheromone Signals in Male and Female Brains. Cell 155, 545 1610-1623. (doi:10.1016/j.cell.2013.11.025). Kohl J, Huoviala P, Jefferis GSXE. 2015. Pheromone processing in Drosophila. Curr Op 547 Neurobiol 34:149-157. (doi:10.1016/j.conb.2015.06.009) Kurtovic A, Widmer A, Dickson BJ. 2007. A single class of olfactory neurons mediates 549 behavioural responses to a Drosophila sex pheromone. Nature 446:542-546. 550 (doi:10.1038/nature05672). Larsson, M. C., Domingos, A. I., Jones, W. D., Chiappe, M. E., Amrein, H., & Vosshall, 552 L. B. (2004). Or83b encodes a broadly expressed odorant receptor essential for 553 Drosophila olfaction. Neuron 43:703-714.(doi:10.1016/j.neuron.2004.08.019) Lebreton S, Grabe V, Omondi AB, Ignell R, Becher PG, Hansson BS, Sachse S, Witzgall 555 P. 2014. Love makes smell blind: mating suppresses pheromone attraction in 556 Drosophila females via OR65a olfactory neurons. Sci Rep 4:7119 557 (doi:10.1038/srep07119) Lebreton S, Trona S, Borrero-Echeverry F, Bilz F, Grabe V, Becher PG, Carlsson MA, 559 Nässel DR, Hansson BS, Sachse S, Witzgall P. 2015. Feeding regulates sex 560 pheromone attraction and courtship in Drosophila females. Sci Rep 5:13132 561 (doi:10.1038/srep13132) Lebreton, S., Borrero-Echeverry, F., Gonzalez, F., Solum, M., Wallin, E.A., Hedenström, 563 E., Hansson, B.S., Gustavsson, A.-L., Bengtsson, M., Birgersson, G., et al. 2017 A 564 Drosophila female pheromone elicits species-specific long-range attraction via an 565 olfactory channel with dual specificity for sex and food. BMC Biol. 15, 88. 566 (doi:10.1186/s12915-017-0427-x). Legendre, A., Miao, X. X., Da Lage, J. L., & Wicker-Thomas, C. (2008). Evolution of a 568 desaturase involved in female pheromonal cuticular hydrocarbon biosynthesis and 569 courtship behavior in Drosophila. Insect biochemistry and molecular biology, 38(2), 570 244-255. (doi:10.1016/j.ibmb.2007.11.005) Ljunggren J, Borrero-Echeverry F, Chakraborty A, Lindblom TU, Hedenström E, Karlsson 572 M, Witzgall P, Bengtsson M. 2019. Yeast volatomes differentially effect larval 573 feeding in an insect herbivore. Appl Environm Microbiol 85: e01761-19 574 (doi:10.1128/AEM.01761-19) Lynch, K.M., Zannini, E., Wilkinson, S., Daenen, L. and Arendt, E.K., 2019. Physiology 576 of acetic acid bacteria and their role in vinegar and fermented beverages. 577 Comprehensive Reviews in Food Science and Food Safety, 18, 587-625. 578 (doi:10.1111/1541-4337.12440) Manoli, D. S., Foss, M., Villella, A., Taylor, B. J., Hall, J. C., & Baker, B. S. 2005. Male580 specific fruitless specifies the neural substrates of Drosophila courtship behaviour. 581 Nature 436:395-400. (doi:10.1038/nature03859) Markow, T. A. (2019). Host use and host shifts in Drosophila. Current Opinion in Insect 583 Science 31:139-145.(doi:10.1016/j.cois.2019.01.006) Merico, A., Sulo, P., Piskur, J. & Compagno, C. (2007) Fermentative lifestyle in yeasts 585 belonging to the Saccharomyces complex. FEBS Journal, 274, 976–989. 586 (doi:10.1111/j.1742-4658.2007.05645.x) Münch D, Galizia CG. 2016. DoOR 2.0 - Comprehensive mapping of Drosophila 588 melanogaster odorant responses. Scientific Reports 6:21841 589 (doi:10.1038/srep21841) Murgier, J., Everaerts, C., Farine, J.P. and Ferveur, J.F., 2019. Live yeast in juvenile 591 diet induces species-specific effects on Drosophila adult behaviour and fitness. 592 Scientific Reports 9,1-12. (doi:10.1038/s41598-019-45140-z) Nakai, J., Ohkura, M., & Imoto, K. (2001). A high signal-to-noise Ca2+ probe composed 594 of a single green fluorescent protein. Nature Biotechnology 19:137- 595 141.(doi:10.1038/84397) Quan AS, Eisen MB (2018) The ecology of the Drosophila-yeast mutualism in wineries. 597 PLoS One 13(5): e0196440. (doi:10.1371/journal.pone.0196440) R Core Team. 2013 R: A Language and Environment for Statistical Computing. Vienna, 599 Austria, R Foundation for Statistical Computing. Robertson HM, Warr CG, Carlson JR. 2003. Molecular evolution of the insect 601 chemoreceptor gene superfamily in Drosophila melanogaster. Proc Natl Acad Sci 602 USA 100:14537-42. (doi:10.1073/pnas.233584710) Ruebenbauer, A., Schlyter, F., Hansson, B.S., Löfstedt, C., Larsson, M.C. 2008. Genetic 604 variability and robustness of host odor preference in Drosophila melanogaster. 605 Curr. Biol. 18: 1438-1443. (doi:10.1016/j.cub.2008.08.062) Ruta V, Datta SR, Vasconcelos ML, Freeland J, Looger LL, Axel R. 2010. A dimorphic 607 pheromone circuit in Drosophila from sensory input to descending output. Nature 608 2010, 468:686-690. (doi:10.1038/nature09554) Sato K and Yamamoto D (2020) Contact-chemosensory evolution underlying 610 reproductive isolation in Drosophila species. Front. Behav. Neurosci. 14:597428. 611 (10.3389/fnbeh.2020.597428) Schaner, A.M., Bartelt, R.J. & Jackson, L.L. 1987. (Z)-11-octadecenyl acetate, an 613 aggregation pheromone inDrosophila simulans . J Chem Ecol 13, 1777–1786 614 (doi:10.1007/BF00980218) Seeholzer, L. F., Seppo, M., Stern, D. L., & Ruta, V. (2018). Evolution of a central 616 neural circuit underlies Drosophila mate preferences. Nature, 559(7715), 564-569. 617 (doi:10.1038/s41586-018-0322-9) Stockinger P, Kvitsiani D, Rotkopf S, Tirian L, Dickson BJ. 2005. Neural circuitry that 619 governs Drosophila male courtship behavior. Cell 121:795-807 620 (doi:10.1016/j.cell.2005.04.026). Strutz, A., Soelter, J., Baschwitz, A., Farhan, A., Grabe, V., Rybak, J., Knaden M, 622 Schmuker M, Hansson BS, Sachse, S. (2014). Decoding odor quality and intensity 623 in the Drosophila brain. Elife, 3, e04147. (doi:10.7554/eLife.04147) Thistle, R., Cameron, P., Ghorayshi, A., Dennison, L., & Scott, K. (2012). Contact 625 chemoreceptors mediate male-male repulsion and male-female attraction during 626 Drosophila courtship. Cell, 149(5), 1140-1151. (doi:10.1016/j.cell.2012.03.045) Toda, H., Zhao, X., & Dickson, B. J. (2012). The Drosophila female aphrodisiac 628 pheromone activates ppk23+ sensory neurons to elicit male courtship behavior. 629 Cell Reports 1:599-607. (doi:10.1016/j.celrep.2012.05.007 Vosshall, L. B., & Stocker, R. F. (2007). Molecular architecture of smell and taste in 631 Drosophila. Annu. Rev. Neurosci. 30:505-533. 632 (doi:10.1146/annurev.neuro.30.051606.094306) Wilson RI. 2013. Early olfactory processing in Drosophila: mechanisms and principles. 634 Annu Rev Neurosci 36:217-241 (doi:10.1146/annurev-neuro-062111-150533) Yamamoto, D., & Koganezawa, M. (2013). Genes and circuits of courtship behaviour in 636 Drosophila males. Nature Reviews Neuroscience 14:681-692. 637 (doi:10.1038/nrn3567) Atribución-NoComercial-CompartirIgual 4.0 Internacional http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf C.I Tibaitatá Cold Sprimg Harbor Laboratory - CSH BioRxiv; (2021): BioRxiv (Ene.);p. 1 - 22.

The female pheromone (Z)-4-undecenal mediates flight attraction and courtship in Drosophila melanogaster

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