Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants
Phosphorus is limiting for growth of maize plants, and because of that use of fertilizers like Rock Phosphate has been proposed. However, direct use of Rock Phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria...
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Universidad Nacional de Colombia
2025
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| Acceso en línea: | https://repositorio.unal.edu.co/handle/unal/71393 http://hdl.handle.net/20.500.12324/40866 https://repositorio.unal.edu.co/handle/unal/71393 |
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RepoAGROSAVIA40866 |
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Corporación Colombiana de Investigación Agropecuaria |
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Repositorio AGROSAVIA |
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Inglés |
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Enfermedades de las plantas - H20 Zea mays Bacteria Fosfato Compuesto inorgánico Transitorios http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_35986 http://aims.fao.org/aos/agrovoc/c_3881 |
| spellingShingle |
Enfermedades de las plantas - H20 Zea mays Bacteria Fosfato Compuesto inorgánico Transitorios http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_35986 http://aims.fao.org/aos/agrovoc/c_3881 López Ortega, Mónica Del Pilar Criollo Campos, Paola Jimena Gómez Vargas, Ruth Milena Camelo Runsinque, Mauricio Estrada Bonilla, Germán Garrido Rubiano, María Fernanda Bonilla Buitrago, Ruth Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| description |
Phosphorus is limiting for growth of maize plants, and because of that use of fertilizers like Rock Phosphate has been proposed. However, direct use of Rock Phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria were evaluated as phosphate-solubilizing bacteria, for their production of indolic compounds and for their effects on growth of maize plants. Strains of the genera Azosporillum, Azotobacter, Rhizobium and Klebsiella, were quantitatively evaluated for solubilization of Ca3(PO4)2 and rock phosphate as a single source of phosphorous in SRS culture media. Additionally, the phosphatase enzyme activity was quantified at pH 5.0, 7.0 and 8.0 using p-nitrophenyl phosphate, and production of indolic compound was determined by colorimetric quantification. The effect of inoculation of bacteria on maize was determined in a completely randomized greenhouse experiment where root and shoot dry weights and phosphorus content were assessed. Results showed that strain C50 produced 107.2 mg .L-1 of available-P after 12 days of fermentation, and AC10 strain had the highest phosphatase activity at pH 8 with 12.7 mg of p-nitrophenol mL .h-1. All strains synthetized indolic compounds, and strain AV5 strain produced the most at 63.03 µg .mL-1. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Hence, use of diazotrphic phosphate-solubilizing bacteria may represent an alternative technology for fertilization systems in maize plants. |
| format |
article |
| author |
López Ortega, Mónica Del Pilar Criollo Campos, Paola Jimena Gómez Vargas, Ruth Milena Camelo Runsinque, Mauricio Estrada Bonilla, Germán Garrido Rubiano, María Fernanda Bonilla Buitrago, Ruth |
| author_facet |
López Ortega, Mónica Del Pilar Criollo Campos, Paola Jimena Gómez Vargas, Ruth Milena Camelo Runsinque, Mauricio Estrada Bonilla, Germán Garrido Rubiano, María Fernanda Bonilla Buitrago, Ruth |
| author_sort |
López Ortega, Mónica Del Pilar |
| title |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_short |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_full |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_fullStr |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_full_unstemmed |
Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| title_sort |
characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants |
| publisher |
Universidad Nacional de Colombia |
| publishDate |
2025 |
| url |
https://repositorio.unal.edu.co/handle/unal/71393 http://hdl.handle.net/20.500.12324/40866 https://repositorio.unal.edu.co/handle/unal/71393 |
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RepoAGROSAVIA408662025-04-10T03:02:14Z Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants Characterization of diazotrophic phosphate solubilizing bacteria as growth promoters of maize plants López Ortega, Mónica Del Pilar Criollo Campos, Paola Jimena Gómez Vargas, Ruth Milena Camelo Runsinque, Mauricio Estrada Bonilla, Germán Garrido Rubiano, María Fernanda Bonilla Buitrago, Ruth Enfermedades de las plantas - H20 Zea mays Bacteria Fosfato Compuesto inorgánico Transitorios http://aims.fao.org/aos/agrovoc/c_8504 http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_35986 http://aims.fao.org/aos/agrovoc/c_3881 Phosphorus is limiting for growth of maize plants, and because of that use of fertilizers like Rock Phosphate has been proposed. However, direct use of Rock Phosphate is not recommended because of its low availability, so it is necessary to improve it. In this study, a group of diazotrophic bacteria were evaluated as phosphate-solubilizing bacteria, for their production of indolic compounds and for their effects on growth of maize plants. Strains of the genera Azosporillum, Azotobacter, Rhizobium and Klebsiella, were quantitatively evaluated for solubilization of Ca3(PO4)2 and rock phosphate as a single source of phosphorous in SRS culture media. Additionally, the phosphatase enzyme activity was quantified at pH 5.0, 7.0 and 8.0 using p-nitrophenyl phosphate, and production of indolic compound was determined by colorimetric quantification. The effect of inoculation of bacteria on maize was determined in a completely randomized greenhouse experiment where root and shoot dry weights and phosphorus content were assessed. Results showed that strain C50 produced 107.2 mg .L-1 of available-P after 12 days of fermentation, and AC10 strain had the highest phosphatase activity at pH 8 with 12.7 mg of p-nitrophenol mL .h-1. All strains synthetized indolic compounds, and strain AV5 strain produced the most at 63.03 µg .mL-1. These diazotrophic bacteria increased plant biomass up to 39 % and accumulation of phosphorus by 10%. Hence, use of diazotrphic phosphate-solubilizing bacteria may represent an alternative technology for fertilization systems in maize plants. 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