Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia
Potato cyst nematodes (PCN) from the genus Globodera spp. cause major losses in potato (Solanum tuberosum) industry worldwide. Despite their importance, at present little is known about the status of this plant pathogen in cultivated potatoes in Colombia. In this study, a total of 589 samples collec...
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Cold Sprimg Harbor Laboratory (CSH)
2025
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| Online Access: | https://www.biorxiv.org/content/10.1101/2020.10.13.337238v1.full http://hdl.handle.net/20.500.12324/40738 https://doi.org/10.1101/2020.10.13.337238 |
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Corporación Colombiana de Investigación Agropecuaria |
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Enfermedades de las plantas - H20 Solanum tuberosum Enfermedades de las plantas Globodera Nematodos Raíces y tubérculos http://aims.fao.org/aos/agrovoc/c_7221 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_15803 http://aims.fao.org/aos/agrovoc/c_47faeccc |
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Enfermedades de las plantas - H20 Solanum tuberosum Enfermedades de las plantas Globodera Nematodos Raíces y tubérculos http://aims.fao.org/aos/agrovoc/c_7221 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_15803 http://aims.fao.org/aos/agrovoc/c_47faeccc Vallejo, Daniela Rojas, Diego A. Martinez, John A. Marchant, Sergio Holguin, Claudia M. Pérez, Olga Y. Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia |
| description |
Potato cyst nematodes (PCN) from the genus Globodera spp. cause major losses in potato (Solanum tuberosum) industry worldwide. Despite their importance, at present little is known about the status of this plant pathogen in cultivated potatoes in Colombia. In this study, a total of 589 samples collected from 75 geographic localities from nine potato producing departments of Colombia were assayed for the presence of potato cyst nematodes. Fifty-seven percent of samples tested positive for PCN. All populations but one were identified as Globodera pallida, with conspicuous morphometric variation found among populations. Based on phylogenetic analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rRNA gene and D2-D3 expansion segments of the 28S rRNA gene, G. pallida from Colombia formed a monophyletic group closely related to Peruvian populations, with the lowest average number of nucleotide substitutions per site (Dxy= 0.002) and net nucleotide substitutions per site (Da= 0.001), when compared to G. pallida populations from South, North America and Europe. A single sample formed a well-supported subclade along with G. rostochiensis and G. tabacum from Japan, USA and Argentina. To our knowledge this is the first comprehensive survey of Globodera populations from Colombia that includes morphological and genetic data. Our findings on species diversity and phylogenetic relationships of Globodera populations from Colombia may help elucidate the status and distribution of Globodera species, and lead to the development of accurate management strategies for the potato cyst nematodes. |
| format |
article |
| author |
Vallejo, Daniela Rojas, Diego A. Martinez, John A. Marchant, Sergio Holguin, Claudia M. Pérez, Olga Y. |
| author_facet |
Vallejo, Daniela Rojas, Diego A. Martinez, John A. Marchant, Sergio Holguin, Claudia M. Pérez, Olga Y. |
| author_sort |
Vallejo, Daniela |
| title |
Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia |
| title_short |
Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia |
| title_full |
Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia |
| title_fullStr |
Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia |
| title_full_unstemmed |
Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia |
| title_sort |
occurrence and characterization of cyst nematode species (globodera spp.) associated with potato crops in colombia |
| publisher |
Cold Sprimg Harbor Laboratory (CSH) |
| publishDate |
2025 |
| url |
https://www.biorxiv.org/content/10.1101/2020.10.13.337238v1.full http://hdl.handle.net/20.500.12324/40738 https://doi.org/10.1101/2020.10.13.337238 |
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RepoAGROSAVIA407382025-03-04T03:02:41Z Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia Occurrence and characterization of cyst nematode species (Globodera spp.) associated with potato crops in Colombia Vallejo, Daniela Rojas, Diego A. Martinez, John A. Marchant, Sergio Holguin, Claudia M. Pérez, Olga Y. Enfermedades de las plantas - H20 Solanum tuberosum Enfermedades de las plantas Globodera Nematodos Raíces y tubérculos http://aims.fao.org/aos/agrovoc/c_7221 http://aims.fao.org/aos/agrovoc/c_5962 http://aims.fao.org/aos/agrovoc/c_15803 http://aims.fao.org/aos/agrovoc/c_47faeccc Potato cyst nematodes (PCN) from the genus Globodera spp. cause major losses in potato (Solanum tuberosum) industry worldwide. Despite their importance, at present little is known about the status of this plant pathogen in cultivated potatoes in Colombia. In this study, a total of 589 samples collected from 75 geographic localities from nine potato producing departments of Colombia were assayed for the presence of potato cyst nematodes. Fifty-seven percent of samples tested positive for PCN. All populations but one were identified as Globodera pallida, with conspicuous morphometric variation found among populations. Based on phylogenetic analysis of the internal transcribed spacer region (ITS1-5.8S-ITS2) of the rRNA gene and D2-D3 expansion segments of the 28S rRNA gene, G. pallida from Colombia formed a monophyletic group closely related to Peruvian populations, with the lowest average number of nucleotide substitutions per site (Dxy= 0.002) and net nucleotide substitutions per site (Da= 0.001), when compared to G. pallida populations from South, North America and Europe. A single sample formed a well-supported subclade along with G. rostochiensis and G. tabacum from Japan, USA and Argentina. To our knowledge this is the first comprehensive survey of Globodera populations from Colombia that includes morphological and genetic data. Our findings on species diversity and phylogenetic relationships of Globodera populations from Colombia may help elucidate the status and distribution of Globodera species, and lead to the development of accurate management strategies for the potato cyst nematodes. Ministerio de Agricultura y Desarrollo Rural - MADR Papa-Solanum tuberosum 2025-03-03T14:12:44Z 2025-03-03T14:12:44Z 2020-10 2020 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.biorxiv.org/content/10.1101/2020.10.13.337238v1.full http://hdl.handle.net/20.500.12324/40738 https://doi.org/10.1101/2020.10.13.337238 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng BioRxiv 1 1 1 46 Jones JT, Haegeman A, Danchin EGJ, Gaur HS, Helder J, Jones MGK, et al. Top 10 plant-parasitic nematodes in molecular plant pathology. Mol Plant Pathol. 2013;14: 946–961. doi:10.1111/mpp.12057CrossRefPubMedWeb of ScienceGoogle Scholar ↵Subbotin SA, Franco J, Knoetze R, Roubtsova T V., Bostock RM, Cid Del Prado Vera I. 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