Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.

Scopulariopsis fungi genus has 112 recognized species in Index-Fungorum (http://www.indexfungorum.org/Names/Names.asp). Some species are classified as a human opportunistic FFHP (Jagielski et al., 2016). These species are able to produces toxins or carry allergens, which cause health hazards (Wou...

Descripción completa

Detalles Bibliográficos
Autores principales:	Uribe, L., Beltrán Acosta, C., Toloza Moreno, D., Suárez, M., López, M., Rodríguez, T., Braissant, O., Bravo, D.
Formato:	article
Lenguaje:	Inglés
Publicado:	Corporación colombiana de investigación agropecuaria - AGROSAVIA 2025
Materias:	Investigación agropecuaria - A50 Scopulariopsis Trichoderma Calorimetría Transversal http://aims.fao.org/aos/agrovoc/c_24703 http://aims.fao.org/aos/agrovoc/c_15814 http://aims.fao.org/aos/agrovoc/c_28276
Acceso en línea:	http://hdl.handle.net/20.500.12324/40681

id	RepoAGROSAVIA40681
record_format	dspace
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Investigación agropecuaria - A50 Scopulariopsis Trichoderma Calorimetría Transversal http://aims.fao.org/aos/agrovoc/c_24703 http://aims.fao.org/aos/agrovoc/c_15814 http://aims.fao.org/aos/agrovoc/c_28276
spellingShingle	Investigación agropecuaria - A50 Scopulariopsis Trichoderma Calorimetría Transversal http://aims.fao.org/aos/agrovoc/c_24703 http://aims.fao.org/aos/agrovoc/c_15814 http://aims.fao.org/aos/agrovoc/c_28276 Uribe, L. Beltrán Acosta, C. Toloza Moreno, D. Suárez, M. López, M. Rodríguez, T. Braissant, O. Bravo, D. Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
description	Scopulariopsis fungi genus has 112 recognized species in Index-Fungorum (http://www.indexfungorum.org/Names/Names.asp). Some species are classified as a human opportunistic FFHP (Jagielski et al., 2016). These species are able to produces toxins or carry allergens, which cause health hazards (Woudenberg et al. 2017). In environmental sciences, a new species of S. gossypii was categorized an opportunistic pathogen causing leaf interveinal chlorosis and vascular browning of cotton plants (Li et al. 2017). It has been isolated from a wide variety of substrates, containing both hyaline and somewhat pigmented filamentous fungi that normally are saprotrophs (Sandoval-Denis et al. 2013). Control of the pathogen Scopulariopsis sp. is conducted by chemical antifungals such as sertaconazole, terbinafine and tioconazole (Carrillo-Muñoz et al. 2004), however with high costs and low results in environmental conditions. Despite the presence of different Scopulariopsis sp. species facilitating vascular wilt, biological control as a strategy against this fungus is not common. A non-conventional technique that has been used recently in biological control of microorganisms within agro-ecosystems is isothermal microcalorimetry (IMC) (Yao et al. 2007). It is a sensitive method for real-time monitoring and dynamic analysis of chemical, physical and biological processes (Braissant et al. 2010) used to measure metabolic activity of relevant environmental microorganisms (Bravo et al., 2011). This research aims to determine the use of IMC to assess the antagonic activity of Trichoderma. asperellum (Th034 and Th406), the metabolic interaction between Th034, Th406, and Bacillus amyloliquefaciens (Bs006) against Scopulariopsis sp. (Sc002) either independently or in consortium. Using the kinetical parameter data obtained after fitting the Richards equation of growth, an Anova test was performed to compare the variance between the antagonic populations and the target population of Scopulariopsis sp. Using the maximum heat values (Qmax in J.h-1), the fractional inhibitory concentration index of Scopulariopsis sp. (FIC) was calculated as shown in a previous study (Fälcao et al., 2014). The FIC index was above the treshold for antagonic activity using T. asperellum Th406. Using a standardized medium developed in this study, this research demonstrates that Scopulariopsis sp., is inhibited due the metabolic activity of T. asperellum (Th406) measured by IMC with a FIC of 4.88. Thus, the strain Th406 is suggested as a promising microbial agent against Scopulariopsis sp. Sc002.
format	article
author	Uribe, L. Beltrán Acosta, C. Toloza Moreno, D. Suárez, M. López, M. Rodríguez, T. Braissant, O. Bravo, D.
author_facet	Uribe, L. Beltrán Acosta, C. Toloza Moreno, D. Suárez, M. López, M. Rodríguez, T. Braissant, O. Bravo, D.
author_sort	Uribe, L.
title	Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
title_short	Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
title_full	Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
title_fullStr	Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
title_full_unstemmed	Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.
title_sort	isothermal microcalorimetry (imc) to determine antagonistic effects of trichoderma asperellum and bacillus amyloliquefaciens against scopulariopsis sp.
publisher	Corporación colombiana de investigación agropecuaria - AGROSAVIA
publishDate	2025
url	http://hdl.handle.net/20.500.12324/40681
work_keys_str_mv	AT uribel isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT beltranacostac isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT tolozamorenod isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT suarezm isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT lopezm isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT rodriguezt isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT braissanto isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT bravod isothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT uribel posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT beltranacostac posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT tolozamorenod posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT suarezm posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT lopezm posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT rodriguezt posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT braissanto posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp AT bravod posterisothermalmicrocalorimetryimctodetermineantagonisticeffectsoftrichodermaasperellumandbacillusamyloliquefaciensagainstscopulariopsissp
_version_	1842256200317534208
spelling	RepoAGROSAVIA406812025-05-21T17:01:17Z Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp. Poster: Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp. Uribe, L. Beltrán Acosta, C. Toloza Moreno, D. Suárez, M. López, M. Rodríguez, T. Braissant, O. Bravo, D. Investigación agropecuaria - A50 Scopulariopsis Trichoderma Calorimetría Transversal http://aims.fao.org/aos/agrovoc/c_24703 http://aims.fao.org/aos/agrovoc/c_15814 http://aims.fao.org/aos/agrovoc/c_28276 Scopulariopsis fungi genus has 112 recognized species in Index-Fungorum (http://www.indexfungorum.org/Names/Names.asp). Some species are classified as a human opportunistic FFHP (Jagielski et al., 2016). These species are able to produces toxins or carry allergens, which cause health hazards (Woudenberg et al. 2017). In environmental sciences, a new species of S. gossypii was categorized an opportunistic pathogen causing leaf interveinal chlorosis and vascular browning of cotton plants (Li et al. 2017). It has been isolated from a wide variety of substrates, containing both hyaline and somewhat pigmented filamentous fungi that normally are saprotrophs (Sandoval-Denis et al. 2013). Control of the pathogen Scopulariopsis sp. is conducted by chemical antifungals such as sertaconazole, terbinafine and tioconazole (Carrillo-Muñoz et al. 2004), however with high costs and low results in environmental conditions. Despite the presence of different Scopulariopsis sp. species facilitating vascular wilt, biological control as a strategy against this fungus is not common. A non-conventional technique that has been used recently in biological control of microorganisms within agro-ecosystems is isothermal microcalorimetry (IMC) (Yao et al. 2007). It is a sensitive method for real-time monitoring and dynamic analysis of chemical, physical and biological processes (Braissant et al. 2010) used to measure metabolic activity of relevant environmental microorganisms (Bravo et al., 2011). This research aims to determine the use of IMC to assess the antagonic activity of Trichoderma. asperellum (Th034 and Th406), the metabolic interaction between Th034, Th406, and Bacillus amyloliquefaciens (Bs006) against Scopulariopsis sp. (Sc002) either independently or in consortium. Using the kinetical parameter data obtained after fitting the Richards equation of growth, an Anova test was performed to compare the variance between the antagonic populations and the target population of Scopulariopsis sp. Using the maximum heat values (Qmax in J.h-1), the fractional inhibitory concentration index of Scopulariopsis sp. (FIC) was calculated as shown in a previous study (Fälcao et al., 2014). The FIC index was above the treshold for antagonic activity using T. asperellum Th406. Using a standardized medium developed in this study, this research demonstrates that Scopulariopsis sp., is inhibited due the metabolic activity of T. asperellum (Th406) measured by IMC with a FIC of 4.88. Thus, the strain Th406 is suggested as a promising microbial agent against Scopulariopsis sp. Sc002. 2025-02-13T13:37:08Z 2025-02-13T13:37:08Z 2021 2021 article Artículo de divulgación http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.kalorimetrietage.ptb.de/fileadmin/documents/kalorimetrietage/24Kaltage_2021/Posterabstracts/Uribe_Isothermal_microcalorimetry.pdf http://hdl.handle.net/20.500.12324/40681 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng 1 2 Braissant, O., Wirz, D., Göpfert, B., & Daniels, A. U. (2010). Use of isothermal microcalorimetry to monitor microbial activities.FEMS Microbiol Lett, 303(1), 1-8. Bravo, D., Braissant, O., Solokhina, A., Clerc, M., Daniels, A.U., Verrecchia, E. and Junier, P. (2011), Use of an isothermal microcalorimetry assay to characterize microbial oxalotrophic activity. FEMS Microbiol Ecol, 78: 266-274. https://doi.org/10.1111/j.1574-6941.2011.01158.x Carrillo-Muñoz, A. J., Guglietta, A., Palacín, C., Casals, J., Del Valle, O., Guardià, C., Rodríguez, V. and Quindós, G. (2004). In vitro antifungal activity of sertaconazole compared with nine other drugs against 250 clinical isolates of dermatophytes and Scopulariopsis brevicaulis. Chemotherapy, 50(6), 308-313. Cuenca-Estrella, M., Gomez-Lopez, A., Mellado, E., Buitrago, M. J., Monzón, A., & Rodriguez- Tudela, J. L. (2003). Scopulariopsis brevicaulis, a fungal pathogen resistant to broad-spectrum antifungal agents. Antimicrob Agents Chemother, 47(7), 2339-2341. Falcäo, L., Silva‐Werneck, J., Vilarinho, B., da Silva, J., Pomella, A. and Marcellino, L. (2014), Antimicrobial and plant growth‐promoting properties of the cacao endophyte Bacillus subtilis ALB629. J Appl Microbiol, 116: 1584-1592. https://doi.org/10.1111/jam.12485 Jagielski, T., Sandoval-Denis, M., Yu, J., Yao, L., Bakuła, Z., Kalita, J., Skóra, M., Krzysciak, P., Hoog, G, Guarro, J. and Gené, J. (2016). Molecular taxonomy of Scopulariopsis-like fungi with description of new clinical and environmental species. Fungal Biol, 120(4), 586-602. Li, X. L., Ojaghian, M. R., Zhang, J. Z., & Zhu, S. J. (2017). A new species of Scopulariopsis and its synergistic effect on pathogenicity of Verticillium dahliae on cotton plants. Microbiol Res, 201, 12-20. Sandoval-Denis, M., Sutton, D. A., Fothergill, A. W., Cano-Lira, J., Gené, J., Decock, C. A., ... & Guarro, J. (2013). Scopulariopsis, a poorly known opportunistic fungus: spectrum of species in clinical samples and in vitro responses to antifungal drugs. J Clinical Microbiol,51(12), 3937-3943. Woudenberg, J. H. C., Meijer, M., Houbraken, J. and Samson, R. A. (2017). Scopulariopsis and scopulariopsis-like species from indoor environments. Studies Mycol, 88, 1-35. Yao, J., Xu, C., Wang, F., Tian, L., Wang, Y., Chen, H., Yong, Z., M F. Martin., Choi, E., Maskow, T. (2007). An in vitro microcalorimetric method for studying the toxic effect of cadmium on microbial activity of an agricultural soil. Ecotoxicology, 16(7), 503-509. Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf Corporación colombiana de investigación agropecuaria - AGROSAVIA

Isothermal microcalorimetry (IMC) to determine antagonistic effects of Trichoderma asperellum and Bacillus amyloliquefaciens against Scopulariopsis sp.

Ejemplares similares