Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
The genus Azotobacter has been widely used as biofertilizer due to its significant effects on the stimulation and promotion of plant growth in various agricultural species of commercial interest. In order to obtain significantly viable cellular concentration, a scale-up strategy for a liquid fer...
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World Academy of Science, Engineering and Technology
2024
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Acceso en línea: | https://publications.waset.org/10007055/adjustment-and-scale-up-strategy-of-pilot-liquid-fermentation-process-of-azotobacter-sp http://hdl.handle.net/20.500.12324/40244 |
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Corporación Colombiana de Investigación Agropecuaria |
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Repositorio AGROSAVIA |
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Inglés |
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Fertilización - F04 Azotobacter Fermentación Biofertilizante Crecimiento de planta Transversal http://aims.fao.org/aos/agrovoc/c_752 http://aims.fao.org/aos/agrovoc/c_2855 http://aims.fao.org/aos/agrovoc/c_24975 http://aims.fao.org/aos/agrovoc/c_08842b17 |
spellingShingle |
Fertilización - F04 Azotobacter Fermentación Biofertilizante Crecimiento de planta Transversal http://aims.fao.org/aos/agrovoc/c_752 http://aims.fao.org/aos/agrovoc/c_2855 http://aims.fao.org/aos/agrovoc/c_24975 http://aims.fao.org/aos/agrovoc/c_08842b17 Quiroga Cubides, G. Díaz, A. Gómez, M. Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. |
description |
The genus Azotobacter has been widely used as biofertilizer
due to its significant effects on the stimulation and
promotion of plant growth in various agricultural species of
commercial interest. In order to obtain significantly viable cellular
concentration, a scale-up strategy for a liquid fermentation process
(SmF) with two strains of A. chroococcum (named Ac1 and Ac10)
was validated and adjusted at laboratory and pilot scale. A batch
fermentation process under previously defined conditions was carried
out on a biorreactor Infors®, model Minifors of 3.5 L, which served
as a baseline for this research. For the purpose of increasing process
efficiency, the effect of the reduction of stirring speed was evaluated
in combination with a fed-batch-type fermentation laboratory scale.
To reproduce the efficiency parameters obtained, a scale-up strategy
with geometric and fluid dynamic behavior similarities was
evaluated. According to the analysis of variance, this scale-up
strategy did not have significant effect on cellular concentration and
in laboratory and pilot fermentations (Tukey, p > 0.05). Regarding air
consumption, fermentation process at pilot scale showed a reduction
of 23% versus the baseline. The percentage of reduction related to
energy consumption reduction under laboratory and pilot scale
conditions was 96.9% compared with baseline. |
format |
article |
author |
Quiroga Cubides, G. Díaz, A. Gómez, M. |
author_facet |
Quiroga Cubides, G. Díaz, A. Gómez, M. |
author_sort |
Quiroga Cubides, G. |
title |
Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. |
title_short |
Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. |
title_full |
Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. |
title_fullStr |
Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. |
title_full_unstemmed |
Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. |
title_sort |
adjustment and scale-up strategy of pilot liquid fermentation process of azotobacter sp. |
publisher |
World Academy of Science, Engineering and Technology |
publishDate |
2024 |
url |
https://publications.waset.org/10007055/adjustment-and-scale-up-strategy-of-pilot-liquid-fermentation-process-of-azotobacter-sp http://hdl.handle.net/20.500.12324/40244 |
work_keys_str_mv |
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_version_ |
1842256242153619456 |
spelling |
RepoAGROSAVIA402442024-10-18T03:02:41Z Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. Quiroga Cubides, G. Díaz, A. Gómez, M. Fertilización - F04 Azotobacter Fermentación Biofertilizante Crecimiento de planta Transversal http://aims.fao.org/aos/agrovoc/c_752 http://aims.fao.org/aos/agrovoc/c_2855 http://aims.fao.org/aos/agrovoc/c_24975 http://aims.fao.org/aos/agrovoc/c_08842b17 The genus Azotobacter has been widely used as biofertilizer due to its significant effects on the stimulation and promotion of plant growth in various agricultural species of commercial interest. In order to obtain significantly viable cellular concentration, a scale-up strategy for a liquid fermentation process (SmF) with two strains of A. chroococcum (named Ac1 and Ac10) was validated and adjusted at laboratory and pilot scale. A batch fermentation process under previously defined conditions was carried out on a biorreactor Infors®, model Minifors of 3.5 L, which served as a baseline for this research. For the purpose of increasing process efficiency, the effect of the reduction of stirring speed was evaluated in combination with a fed-batch-type fermentation laboratory scale. To reproduce the efficiency parameters obtained, a scale-up strategy with geometric and fluid dynamic behavior similarities was evaluated. According to the analysis of variance, this scale-up strategy did not have significant effect on cellular concentration and in laboratory and pilot fermentations (Tukey, p > 0.05). Regarding air consumption, fermentation process at pilot scale showed a reduction of 23% versus the baseline. The percentage of reduction related to energy consumption reduction under laboratory and pilot scale conditions was 96.9% compared with baseline. Ministerio de Agricultura y Desarrollo Rural - MADR 2024-10-17T15:01:59Z 2024-10-17T15:01:59Z 2017-01 2017 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://publications.waset.org/10007055/adjustment-and-scale-up-strategy-of-pilot-liquid-fermentation-process-of-azotobacter-sp 9195-0263 http://hdl.handle.net/20.500.12324/40244 doi.org/10.5281/zenodo.1130353 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng International Journal of Bioengineering and Life Sciences 11 4 322 330 C. Ervin, D. Ervin, “Factors Affecting the use of soil conservation practices: Hypotheses, Evidence, and Policy Implications,” Land Economics, vol. 58, no. 3, pp. 272-292, Aug. 1982. E. Lutz, S. Pagiola, C. 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Pedroza, “Scale-up, the art of chemical engineering: Pilot plants the passage between the egg and the hen,” Technology, Science, Education, vol. 23, no. 1, pp. 31-39, 2008. C. Hewitt, A. Nienow, “The scaleup of microbial in batch and fedbatch fermentation processes,” Advances in Applied Microbiology, vol. 62, pp. 105-135, 2007. F. Schmidt, “Optimization and scale up of industrial fermentation processes,” Appl. Microbiol Biotechnol, vol. 68, no. 4, pp. 425-435, Oct. 2005. F. Clementi, “Alginate production by Azotobacter vinelandii”, Crit. Reviews in Biotechnol., vol. 17, no. 4, pp. 327-361, 1997. M. Mejía, D. Segura, G. Espín, E. Galindo and C. Peña, “Two-stage fermentation process for alginate production by Azotobacter vinelandii mutant altered in poly-β-hydroxybutyrate (PHB) synthesis”, J. of Applied Microbiol, vol. 108, pp. 55-61, April 2009. O. Damir, M. Pavlecic, B. Santek, S. 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