Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.

The genus Azotobacter has been widely used as biofertilizer due to its significant effects on the stimulation and promotion of plant growth in various agricultural species of commercial interest. In order to obtain significantly viable cellular concentration, a scale-up strategy for a liquid fer...

Descripción completa

Detalles Bibliográficos
Autores principales: Quiroga Cubides, G., Díaz, A., Gómez, M.
Formato: article
Lenguaje:Inglés
Publicado: World Academy of Science, Engineering and Technology 2024
Materias:
Acceso en línea:https://publications.waset.org/10007055/adjustment-and-scale-up-strategy-of-pilot-liquid-fermentation-process-of-azotobacter-sp
http://hdl.handle.net/20.500.12324/40244
id RepoAGROSAVIA40244
record_format dspace
institution Corporación Colombiana de Investigación Agropecuaria
collection Repositorio AGROSAVIA
language Inglés
topic Fertilización - F04
Azotobacter
Fermentación
Biofertilizante
Crecimiento de planta
Transversal
http://aims.fao.org/aos/agrovoc/c_752
http://aims.fao.org/aos/agrovoc/c_2855
http://aims.fao.org/aos/agrovoc/c_24975
http://aims.fao.org/aos/agrovoc/c_08842b17
spellingShingle Fertilización - F04
Azotobacter
Fermentación
Biofertilizante
Crecimiento de planta
Transversal
http://aims.fao.org/aos/agrovoc/c_752
http://aims.fao.org/aos/agrovoc/c_2855
http://aims.fao.org/aos/agrovoc/c_24975
http://aims.fao.org/aos/agrovoc/c_08842b17
Quiroga Cubides, G.
Díaz, A.
Gómez, M.
Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
description The genus Azotobacter has been widely used as biofertilizer due to its significant effects on the stimulation and promotion of plant growth in various agricultural species of commercial interest. In order to obtain significantly viable cellular concentration, a scale-up strategy for a liquid fermentation process (SmF) with two strains of A. chroococcum (named Ac1 and Ac10) was validated and adjusted at laboratory and pilot scale. A batch fermentation process under previously defined conditions was carried out on a biorreactor Infors®, model Minifors of 3.5 L, which served as a baseline for this research. For the purpose of increasing process efficiency, the effect of the reduction of stirring speed was evaluated in combination with a fed-batch-type fermentation laboratory scale. To reproduce the efficiency parameters obtained, a scale-up strategy with geometric and fluid dynamic behavior similarities was evaluated. According to the analysis of variance, this scale-up strategy did not have significant effect on cellular concentration and in laboratory and pilot fermentations (Tukey, p > 0.05). Regarding air consumption, fermentation process at pilot scale showed a reduction of 23% versus the baseline. The percentage of reduction related to energy consumption reduction under laboratory and pilot scale conditions was 96.9% compared with baseline.
format article
author Quiroga Cubides, G.
Díaz, A.
Gómez, M.
author_facet Quiroga Cubides, G.
Díaz, A.
Gómez, M.
author_sort Quiroga Cubides, G.
title Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
title_short Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
title_full Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
title_fullStr Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
title_full_unstemmed Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp.
title_sort adjustment and scale-up strategy of pilot liquid fermentation process of azotobacter sp.
publisher World Academy of Science, Engineering and Technology
publishDate 2024
url https://publications.waset.org/10007055/adjustment-and-scale-up-strategy-of-pilot-liquid-fermentation-process-of-azotobacter-sp
http://hdl.handle.net/20.500.12324/40244
work_keys_str_mv AT quirogacubidesg adjustmentandscaleupstrategyofpilotliquidfermentationprocessofazotobactersp
AT diaza adjustmentandscaleupstrategyofpilotliquidfermentationprocessofazotobactersp
AT gomezm adjustmentandscaleupstrategyofpilotliquidfermentationprocessofazotobactersp
_version_ 1842256242153619456
spelling RepoAGROSAVIA402442024-10-18T03:02:41Z Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. Adjustment and Scale-Up Strategy of Pilot Liquid Fermentation Process of Azotobacter sp. Quiroga Cubides, G. Díaz, A. Gómez, M. Fertilización - F04 Azotobacter Fermentación Biofertilizante Crecimiento de planta Transversal http://aims.fao.org/aos/agrovoc/c_752 http://aims.fao.org/aos/agrovoc/c_2855 http://aims.fao.org/aos/agrovoc/c_24975 http://aims.fao.org/aos/agrovoc/c_08842b17 The genus Azotobacter has been widely used as biofertilizer due to its significant effects on the stimulation and promotion of plant growth in various agricultural species of commercial interest. In order to obtain significantly viable cellular concentration, a scale-up strategy for a liquid fermentation process (SmF) with two strains of A. chroococcum (named Ac1 and Ac10) was validated and adjusted at laboratory and pilot scale. A batch fermentation process under previously defined conditions was carried out on a biorreactor Infors®, model Minifors of 3.5 L, which served as a baseline for this research. For the purpose of increasing process efficiency, the effect of the reduction of stirring speed was evaluated in combination with a fed-batch-type fermentation laboratory scale. To reproduce the efficiency parameters obtained, a scale-up strategy with geometric and fluid dynamic behavior similarities was evaluated. According to the analysis of variance, this scale-up strategy did not have significant effect on cellular concentration and in laboratory and pilot fermentations (Tukey, p > 0.05). Regarding air consumption, fermentation process at pilot scale showed a reduction of 23% versus the baseline. The percentage of reduction related to energy consumption reduction under laboratory and pilot scale conditions was 96.9% compared with baseline. Ministerio de Agricultura y Desarrollo Rural - MADR 2024-10-17T15:01:59Z 2024-10-17T15:01:59Z 2017-01 2017 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://publications.waset.org/10007055/adjustment-and-scale-up-strategy-of-pilot-liquid-fermentation-process-of-azotobacter-sp 9195-0263 http://hdl.handle.net/20.500.12324/40244 doi.org/10.5281/zenodo.1130353 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng International Journal of Bioengineering and Life Sciences 11 4 322 330 C. Ervin, D. Ervin, “Factors Affecting the use of soil conservation practices: Hypotheses, Evidence, and Policy Implications,” Land Economics, vol. 58, no. 3, pp. 272-292, Aug. 1982. E. Lutz, S. Pagiola, C. Reiche, “The costs and benefits of soil conservation: The farmer´s Viewpoint,” The Word Bank Research Observer, vol. 9, no. 2, pp. 273-295, July, 1994. M. Yussefi, H. Willer, “Organic Farming Worldwide 2007: Overview and main statistics,” in The world of organic agriculture, Bonn: IFOAM & FiBL, 2007, pp. 9-22. C. Cruz, L. Barrero, F. Rodríguez, “Analysis of bioprospecting processes in Colombia,” in Bioprospecting for the development of the agricultural sector of Colombia, Mosquera, 2012, pp. 21-30. M. Camelo, “Technological development of a biofertilizer based on the diazotrophic bacterium Azotobacter chroococcum,” Master’s Thesis, Military University Nueva Granada, Bogotá, 2010, pp. 8-13. N. Mrkovacki, V. Millic, “Use of Azotobacter chrocooccum as potentially useful in agricultural applications,” Annals of Microbiol., vol. 51, pp. 145-158, 2001 M. Brown, “Role of Azotobacter paspaliin Association with Paspalum notatum,” J. Applied Bacteriol., vol. 40, no. 3, pp. 341-348, June 1976 A. Moreno-Galván, D. Rojas, R. Bonilla, “Sequential statistical design application in identification of Azotobacter chroococcum AC1 nutritional sources,” J. CORPOICA - Agricultural Science and Technology, vol. 11, no. 2, pp. 151-158, Nov. 2011 A. Lara, L. Palomares, O. Ramírez, “Bioreactor scale up,” in Encyclopedia of cell Technol., 2000, pp. 1-22 J. Martinko, M. T. Madigan, K. S. Bender, D. H. Buckley, D. A. Stahl, T. Brock, Brock: biology of microorganisms, Madrid: Pearson, 12th edition, 2009. J. Chimero, “Estudio sobre la depuración de los lixiviados de RSU con cenizas volátiles zeolitizadas,” Master’s Thesis, 2006. G. L. Turner, A. H. Gibson, “Measurement of nitrogen fixation by indirect means,” in Methods for Evaluating Biological Nitrogen Fixation, Wiley, Ed., Chichester, 1980. pp. 111–139. A. Díaz-Barrera, A. Aguirre, J. Berrios, F. Acevedo, “Continuous cultures for alginate production by Azotobacter vinelandii growing at different oxygen uptaken rates,” Process Biochemistry, vol. 46, no. 9, pp. 1879-1883, June 2011. C. Then, Z. Othman, W. Mustapha, M. Sarmidi, R. Aziz, H. El Enshasy, “Production of alginate by Azotobacter vinelandii in semi-industrial scale using batch and fed-batch cultivations systems,” J. of Adv. Scien. Research, vol. 3, no. 4, pp. 45-50, Jan. 2012. M. Doyle, L. R. Beuchat, T. J. Montville, Microbiología de los alimentos. Fundamentos y fronteras, 2ª ed., España: Acribia Ed., 2001, pp. 773-785. R. M. Maier, I. L. Pepper, C. P. Gerba, “Bacterial Growth,” in Environmental Microbiology, 3rd ed., San Diego, CA: Academic Press Inc, 2009, pp. 40. R. Perry, Handbook of Chemical Engineering, 4th ed., McGraw-Hill Ed., 2001. P. Harriott, “Nonideal Flow” in Chemical Reactor Design. 1st ed. New York: CRC Press Ed., 2003, pp. 231-262. A. Anaya, H. Pedroza, “Scale-up, the art of chemical engineering: Pilot plants the passage between the egg and the hen,” Technology, Science, Education, vol. 23, no. 1, pp. 31-39, 2008. C. Hewitt, A. Nienow, “The scaleup of microbial in batch and fedbatch fermentation processes,” Advances in Applied Microbiology, vol. 62, pp. 105-135, 2007. F. Schmidt, “Optimization and scale up of industrial fermentation processes,” Appl. Microbiol Biotechnol, vol. 68, no. 4, pp. 425-435, Oct. 2005. F. Clementi, “Alginate production by Azotobacter vinelandii”, Crit. Reviews in Biotechnol., vol. 17, no. 4, pp. 327-361, 1997. M. Mejía, D. Segura, G. Espín, E. Galindo and C. Peña, “Two-stage fermentation process for alginate production by Azotobacter vinelandii mutant altered in poly-β-hydroxybutyrate (PHB) synthesis”, J. of Applied Microbiol, vol. 108, pp. 55-61, April 2009. O. Damir, M. Pavlecic, B. Santek, S. Novak, “Cultivation of the bacterium Azotobacter chroococcum for preparation of biofertilizers,” African Journal of Biotechnology, vol. 10, no. 16, pp. 3104-3111, April 2011. C. Pozo, M. Martínez-Toledo, B. Rodelas, J. González-López, “Effects of culture conditions on the production of polyhydroxyalkanoates by Azotobacter chroococcum H23 in media containing a high concentration of alpechin as primary carbon source,” J. of Biotechol., vol. 97, no. 2, pp. 125-131, 2002. C. Reyes, C. Peña, E. Galindo, “Reproducing shake flask performance in stirred fermentors: production of alginates by Azotobacter vinelandii,” J. of Biotechol., vol. 105, no. 1-2, pp. 189-198, Nov. 2003. C. Peña, M. Millán, E. Galindo, “Production of alginate by Azotobacter vinelandii in a stirred fermentor simulating the evolution of power input observed in shake flasks,” Process Biochemistry, vol. 43, pp. 775-778, July 2008. B. Juárez, J. Martínez-Toledo, J. González-López, “Growth of Azotobacter chroococcum in chemically defined media containing phydroxybenzoic acid and protocatechuic acid,” Chemosphere, vol. 59, no. 3, pp. 136-1365, Dec. 2001. R. Quintero, Biochemical Engineering. Theory and applications, Mexico: Ediciones Alhambra Mexicana, 1981. L. Maranga, A. Cunha, J. Clemente, P. Cruz, M. Carrondo, “Scale‐up of virus‐like particles production: effects of sparging, agitation and bioreactor scale on cell growth, infection kinetics and productivity,” J Biotechnol, vol. 107, no. 1 pp. 55‐64, Jan. 2004. Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf World Academy of Science, Engineering and Technology Revista ICA; Vol. 12, Núm. 1 (1977): Revista ICA (Marzo);p. 55 -66.