Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk
Raw cow milk is considered one of the most important vehicles for pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. These three bacteria are responsible for foodborne diseases. Routine microbiological methods to detect these microorganisms in cow mil...
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Pontificia Universidad Javeriana
2024
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| Acceso en línea: | https://revistas.javeriana.edu.co/index.php/scientarium/article/view/20519 http://hdl.handle.net/20.500.12324/40194 https://doi.org/10.11144/Javeriana.SC24-1.aoam |
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RepoAGROSAVIA40194 |
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dspace |
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Corporación Colombiana de Investigación Agropecuaria |
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Repositorio AGROSAVIA |
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Inglés |
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Ganadería - L01 Ganado de leche Enfermedad transmitida por alimentos Seguridad alimentaria Higiene de la leche Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_2108 http://aims.fao.org/aos/agrovoc/c_24842 http://aims.fao.org/aos/agrovoc/c_10967 http://aims.fao.org/aos/agrovoc/c_2110 |
| spellingShingle |
Ganadería - L01 Ganado de leche Enfermedad transmitida por alimentos Seguridad alimentaria Higiene de la leche Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_2108 http://aims.fao.org/aos/agrovoc/c_24842 http://aims.fao.org/aos/agrovoc/c_10967 http://aims.fao.org/aos/agrovoc/c_2110 Rocio Patiño, Burbano Carrascal, Ana Karina Parra Arango, Jorge Luis Rodríguez Bautista, José Luis Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk |
| description |
Raw cow milk is considered one of the most important vehicles for
pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and
Listeria monocytogenes. These three bacteria are responsible for foodborne
diseases. Routine microbiological methods to detect these microorganisms in
cow milk can be complicated and time consuming. The aim of this work was
to evaluate a method to simultaneously detect Salmonella spp., Escherichia coli
O157:H7, and Listeria monocytogenes in experimentally contaminated cow
milk. The assessed method combined a standard microbiological culture
step, using a pre-enrichment medium that favors the growth of the
three focal microorganisms: SEL broth, followed by a single PCR assay.
A total of 43 interference bacterial strains were used to evaluate the
method’s specificity. The detection rate for the microbiological method
with standard culture media was 10 UFC/mL, and that of the PCR
detection, following pre-enrichment in SEL broth, was 10 UFC/mL for
S. enterica and L. monocytogenes and between 1 and 5 UFC/mL for E. coli
O157:H7. The PCR method showed specificity for the reference strains.
Simultaneous detection by multiple PCR using SEL broth was successful for
the detection of S. enterica, E. coli O157:H7, and L. monocytogenes in samples
of experimentally contaminated cow milk, featuring both a high detection
rate and a high specificity. This approach promises to be a feasible routine
procedure when testing milk samples in industry and public health control
setups. |
| format |
article |
| author |
Rocio Patiño, Burbano Carrascal, Ana Karina Parra Arango, Jorge Luis Rodríguez Bautista, José Luis |
| author_facet |
Rocio Patiño, Burbano Carrascal, Ana Karina Parra Arango, Jorge Luis Rodríguez Bautista, José Luis |
| author_sort |
Rocio Patiño, Burbano |
| title |
Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk |
| title_short |
Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk |
| title_full |
Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk |
| title_fullStr |
Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk |
| title_full_unstemmed |
Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk |
| title_sort |
assessment of a multiplex detection method for salmonella enterica, escherichia coli o157:h7, and listeria monocytogenes in cow milk |
| publisher |
Pontificia Universidad Javeriana |
| publishDate |
2024 |
| url |
https://revistas.javeriana.edu.co/index.php/scientarium/article/view/20519 http://hdl.handle.net/20.500.12324/40194 https://doi.org/10.11144/Javeriana.SC24-1.aoam |
| work_keys_str_mv |
AT rociopatinoburbano assessmentofamultiplexdetectionmethodforsalmonellaentericaescherichiacolio157h7andlisteriamonocytogenesincowmilk AT carrascalanakarina assessmentofamultiplexdetectionmethodforsalmonellaentericaescherichiacolio157h7andlisteriamonocytogenesincowmilk AT parraarangojorgeluis assessmentofamultiplexdetectionmethodforsalmonellaentericaescherichiacolio157h7andlisteriamonocytogenesincowmilk AT rodriguezbautistajoseluis assessmentofamultiplexdetectionmethodforsalmonellaentericaescherichiacolio157h7andlisteriamonocytogenesincowmilk |
| _version_ |
1842255536081338368 |
| spelling |
RepoAGROSAVIA401942024-10-04T03:00:26Z Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk Assessment of a multiplex detection method for Salmonella enterica, Escherichia coli O157:H7, and Listeria monocytogenes in cow milk Rocio Patiño, Burbano Carrascal, Ana Karina Parra Arango, Jorge Luis Rodríguez Bautista, José Luis Ganadería - L01 Ganado de leche Enfermedad transmitida por alimentos Seguridad alimentaria Higiene de la leche Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_2108 http://aims.fao.org/aos/agrovoc/c_24842 http://aims.fao.org/aos/agrovoc/c_10967 http://aims.fao.org/aos/agrovoc/c_2110 Raw cow milk is considered one of the most important vehicles for pathogenic bacteria like Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes. These three bacteria are responsible for foodborne diseases. Routine microbiological methods to detect these microorganisms in cow milk can be complicated and time consuming. The aim of this work was to evaluate a method to simultaneously detect Salmonella spp., Escherichia coli O157:H7, and Listeria monocytogenes in experimentally contaminated cow milk. The assessed method combined a standard microbiological culture step, using a pre-enrichment medium that favors the growth of the three focal microorganisms: SEL broth, followed by a single PCR assay. A total of 43 interference bacterial strains were used to evaluate the method’s specificity. The detection rate for the microbiological method with standard culture media was 10 UFC/mL, and that of the PCR detection, following pre-enrichment in SEL broth, was 10 UFC/mL for S. enterica and L. monocytogenes and between 1 and 5 UFC/mL for E. coli O157:H7. The PCR method showed specificity for the reference strains. Simultaneous detection by multiple PCR using SEL broth was successful for the detection of S. enterica, E. coli O157:H7, and L. monocytogenes in samples of experimentally contaminated cow milk, featuring both a high detection rate and a high specificity. This approach promises to be a feasible routine procedure when testing milk samples in industry and public health control setups. Corporación Colombiana de Investigación Agropecuaria - (AGROSAVIA) Ministerio de Agricultura y Desarrollo Rural - MADR Ganado de leche-Ganadería leche 2024-10-03T16:28:33Z 2024-10-03T16:28:33Z 2019-04-08 2019 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://revistas.javeriana.edu.co/index.php/scientarium/article/view/20519 2027-1352 http://hdl.handle.net/20.500.12324/40194 https://doi.org/10.11144/Javeriana.SC24-1.aoam reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Universitas Scientiarum 24 1 277 294 Middleton J, Saeman A, Fox L, Lombard J, Hogan J, Smith K J. 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Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ application/pdf application/pdf Colombia Pontificia Universidad Javeriana Universitas Scientiarum; Vol. 24, Núm. 1 (2019): Universitas Scientiarum (Apr.);p. 277 - 294. |