Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.)

Anthurium andraeanum (Hort.) is an important ornamental in the tropical cut-flower industry. However, there is currently not enough information to establish a clear connection between the genetic model(s) proposed and the putative genes involved in the differentiation between colors. In this study,...

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Main Authors:	Osorio Guarín, Jaime A., Gopaulchan, David, Quackenbush, Corey, Lennon, Adrian M., Umaharan, Pathmanathan, Cornejo, Omar E.
Format:	article
Language:	Inglés
Published:	Cold Sprimg Harbor Laboratory (CSH) 2024
Subjects:	Flor cortada Industria Genética Transversal http://aims.fao.org/aos/agrovoc/c_16485 http://aims.fao.org/aos/agrovoc/c_3848 http://aims.fao.org/aos/agrovoc/c_3222
Online Access:	https://www.biorxiv.org/content/10.1101/2020.12.29.424726v1 http://hdl.handle.net/20.500.12324/40193 https://doi.org/10.1101/2020.12.29.424726

id	RepoAGROSAVIA40193
record_format	dspace
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Flor cortada Industria Genética Genética Transversal http://aims.fao.org/aos/agrovoc/c_16485 http://aims.fao.org/aos/agrovoc/c_3848 http://aims.fao.org/aos/agrovoc/c_3222
spellingShingle	Flor cortada Industria Genética Genética Transversal http://aims.fao.org/aos/agrovoc/c_16485 http://aims.fao.org/aos/agrovoc/c_3848 http://aims.fao.org/aos/agrovoc/c_3222 Osorio Guarín, Jaime A. Gopaulchan, David Quackenbush, Corey Lennon, Adrian M. Umaharan, Pathmanathan Cornejo, Omar E. Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.)
description	Anthurium andraeanum (Hort.) is an important ornamental in the tropical cut-flower industry. However, there is currently not enough information to establish a clear connection between the genetic model(s) proposed and the putative genes involved in the differentiation between colors. In this study, 18 cDNA libraries related to the spathe color and developmental stages of A. andraeanum cut-flowers were characterized by transcriptome sequencing technology. For the de novo transcriptome, a total of 114,334,082 primary sequence reads were obtained from the Illumina sequencer and were assembled into 151,652 unigenes. Approximately 58,476 transcripts were generated and used for comparative transcriptome analysis between three varieties that differ in spathe color (‘Sasha’ (white), ‘Honduras’ (red), and ‘Rapido’ (purple)). A large number of differentially expressed genes (8,324) that were potentially involved in multiple biological and metabolic pathways were identified, including the flavonoid and anthocyanin biosynthetic pathways. Our results showed that chalcone synthase (CHS) and flavonoid 3’-hydroxylase (F3’H) were the main genes differentially expressed in the white/red/purple comparison. We also identified a differentially expressed cytochrome P450 in the late developmental stage of the purple spathe that appeared to determine the difference between the red- and purple-colored spathes. Additionally, putative MYB-domain protein candidates that could be responsible for the control of the biosynthetic pathway were identified. The results provided basic sequence information for future research on spathe color, which have important implications for breeding strategies in this ornamental.
format	article
author	Osorio Guarín, Jaime A. Gopaulchan, David Quackenbush, Corey Lennon, Adrian M. Umaharan, Pathmanathan Cornejo, Omar E.
author_facet	Osorio Guarín, Jaime A. Gopaulchan, David Quackenbush, Corey Lennon, Adrian M. Umaharan, Pathmanathan Cornejo, Omar E.
author_sort	Osorio Guarín, Jaime A.
title	Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.)
title_short	Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.)
title_full	Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.)
title_fullStr	Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.)
title_full_unstemmed	Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.)
title_sort	comparative transcriptomic analysis reveals key components controlling spathe color in anthurium andraeanum (hort.)
publisher	Cold Sprimg Harbor Laboratory (CSH)
publishDate	2024
url	https://www.biorxiv.org/content/10.1101/2020.12.29.424726v1 http://hdl.handle.net/20.500.12324/40193 https://doi.org/10.1101/2020.12.29.424726
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spelling	RepoAGROSAVIA401932024-10-04T03:02:46Z Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.) Comparative transcriptomic analysis reveals key components controlling spathe color in Anthurium andraeanum (Hort.) Osorio Guarín, Jaime A. Gopaulchan, David Quackenbush, Corey Lennon, Adrian M. Umaharan, Pathmanathan Cornejo, Omar E. Flor cortada Industria Genética Genética Transversal http://aims.fao.org/aos/agrovoc/c_16485 http://aims.fao.org/aos/agrovoc/c_3848 http://aims.fao.org/aos/agrovoc/c_3222 Anthurium andraeanum (Hort.) is an important ornamental in the tropical cut-flower industry. However, there is currently not enough information to establish a clear connection between the genetic model(s) proposed and the putative genes involved in the differentiation between colors. In this study, 18 cDNA libraries related to the spathe color and developmental stages of A. andraeanum cut-flowers were characterized by transcriptome sequencing technology. For the de novo transcriptome, a total of 114,334,082 primary sequence reads were obtained from the Illumina sequencer and were assembled into 151,652 unigenes. Approximately 58,476 transcripts were generated and used for comparative transcriptome analysis between three varieties that differ in spathe color (‘Sasha’ (white), ‘Honduras’ (red), and ‘Rapido’ (purple)). A large number of differentially expressed genes (8,324) that were potentially involved in multiple biological and metabolic pathways were identified, including the flavonoid and anthocyanin biosynthetic pathways. Our results showed that chalcone synthase (CHS) and flavonoid 3’-hydroxylase (F3’H) were the main genes differentially expressed in the white/red/purple comparison. We also identified a differentially expressed cytochrome P450 in the late developmental stage of the purple spathe that appeared to determine the difference between the red- and purple-colored spathes. Additionally, putative MYB-domain protein candidates that could be responsible for the control of the biosynthetic pathway were identified. The results provided basic sequence information for future research on spathe color, which have important implications for breeding strategies in this ornamental. 2024-10-03T16:13:47Z 2024-10-03T16:13:47Z 2020-12-30 2020 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 https://www.biorxiv.org/content/10.1101/2020.12.29.424726v1 http://hdl.handle.net/20.500.12324/40193 https://doi.org/10.1101/2020.12.29.424726 reponame:Biblioteca Digital Agropecuaria de Colombia instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng BioRxiv 1 30 Andrews, S., F. Krueger, A. Segonds-Pichon, L. Biggins, C. Krueger, and S. Wingett. 2012. 455 FastQC: A Quality Control Tool for High Throughput Sequence Data. 456 http://www.bioinformatics.bbsrc.ac.uk/projects/fastqc/. (accessed August 25, 2019). Benjamini, Y., and Y. Hochberg. 1995. 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