Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division

Fission yeast cells prevent mitotic entry until a threshold cell surface area is reached. The protein kinase Cdr2 contributes to this size control system by forming multiprotein nodes that inhibit Wee1 at the medial cell cortex. Cdr2 node anchoring at the cell cortex is not fully understood. Through...

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Main Authors:	Opalko, Hannah E., Miller, Kristi E., Kim, Hyun Soo, Vargas Garcia, Cesar Augusto, Singh, Abhyudai, Keogh, Michael Christopher, Moseley, James B.
Format:	article
Language:	Español
Published:	Rockfeller University Press 2024
Subjects:	Investigación agropecuaria - A50 Genética molecular Ciclo celular División celular Procesos biológicos Transversal http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_37218 http://aims.fao.org/aos/agrovoc/c_1413 http://aims.fao.org/aos/agrovoc/c_330999
Online Access:	https://doi.org/10.1083/jcb.202109152 http://hdl.handle.net/20.500.12324/38936

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record_format	dspace
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Español
topic	Investigación agropecuaria - A50 Genética molecular Ciclo celular División celular Procesos biológicos Transversal http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_37218 http://aims.fao.org/aos/agrovoc/c_1413 http://aims.fao.org/aos/agrovoc/c_330999
spellingShingle	Investigación agropecuaria - A50 Genética molecular Ciclo celular División celular Procesos biológicos Transversal http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_37218 http://aims.fao.org/aos/agrovoc/c_1413 http://aims.fao.org/aos/agrovoc/c_330999 Opalko, Hannah E. Miller, Kristi E. Kim, Hyun Soo Vargas Garcia, Cesar Augusto Singh, Abhyudai Keogh, Michael Christopher Moseley, James B. Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
description	Fission yeast cells prevent mitotic entry until a threshold cell surface area is reached. The protein kinase Cdr2 contributes to this size control system by forming multiprotein nodes that inhibit Wee1 at the medial cell cortex. Cdr2 node anchoring at the cell cortex is not fully understood. Through a genomic screen, we identified the conserved GTPase Arf6 as a component of Cdr2 signaling. Cells lacking Arf6 failed to divide at a threshold surface area and instead shifted to volume-based divisions at increased overall size. Arf6 stably localized to Cdr2 nodes in its GTP-bound but not GDP-bound state, and its guanine nucleotide exchange factor (GEF), Syt22, was required for both Arf6 node localization and proper size at division. In arf6<U+0394> mutants, Cdr2 nodes detached from the membrane and exhibited increased dynamics. These defects were enhanced when arf6<U+0394> was combined with other node mutants. Our work identifies a regulated anchor for Cdr2 nodes that is required for cells to sense surface area.
format	article
author	Opalko, Hannah E. Miller, Kristi E. Kim, Hyun Soo Vargas Garcia, Cesar Augusto Singh, Abhyudai Keogh, Michael Christopher Moseley, James B.
author_facet	Opalko, Hannah E. Miller, Kristi E. Kim, Hyun Soo Vargas Garcia, Cesar Augusto Singh, Abhyudai Keogh, Michael Christopher Moseley, James B.
author_sort	Opalko, Hannah E.
title	Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
title_short	Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
title_full	Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
title_fullStr	Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
title_full_unstemmed	Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division
title_sort	arf6 anchors cdr2 nodes at the cell cortex to control cell size at division
publisher	Rockfeller University Press
publishDate	2024
url	https://doi.org/10.1083/jcb.202109152 http://hdl.handle.net/20.500.12324/38936
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spelling	RepoAGROSAVIA389362024-02-24T03:02:34Z Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division Opalko, Hannah E. Miller, Kristi E. Kim, Hyun Soo Vargas Garcia, Cesar Augusto Singh, Abhyudai Keogh, Michael Christopher Moseley, James B. Investigación agropecuaria - A50 Genética molecular Ciclo celular División celular Procesos biológicos Transversal http://aims.fao.org/aos/agrovoc/c_27577 http://aims.fao.org/aos/agrovoc/c_37218 http://aims.fao.org/aos/agrovoc/c_1413 http://aims.fao.org/aos/agrovoc/c_330999 Fission yeast cells prevent mitotic entry until a threshold cell surface area is reached. The protein kinase Cdr2 contributes to this size control system by forming multiprotein nodes that inhibit Wee1 at the medial cell cortex. Cdr2 node anchoring at the cell cortex is not fully understood. Through a genomic screen, we identified the conserved GTPase Arf6 as a component of Cdr2 signaling. Cells lacking Arf6 failed to divide at a threshold surface area and instead shifted to volume-based divisions at increased overall size. Arf6 stably localized to Cdr2 nodes in its GTP-bound but not GDP-bound state, and its guanine nucleotide exchange factor (GEF), Syt22, was required for both Arf6 node localization and proper size at division. In arf6<U+0394> mutants, Cdr2 nodes detached from the membrane and exhibited increased dynamics. These defects were enhanced when arf6<U+0394> was combined with other node mutants. 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Arf6 anchors Cdr2 nodes at the cell cortex to control cell size at division

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