NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus
NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inac...
Autor principal: | |
---|---|
Otros Autores: | |
Formato: | doctoralThesis |
Lenguaje: | Inglés |
Publicado: |
Syracuse University
2019
|
Materias: | |
Acceso en línea: | http://hdl.handle.net/20.500.12324/35011 |
id |
RepoAGROSAVIA35011 |
---|---|
record_format |
dspace |
spelling |
RepoAGROSAVIA350112023-02-21T01:02:32Z NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus Ossa Ramírez, Faisury Garza Anthony Fisiología de la planta nutrición - F61 Protección de plantas aspectos generales - H01 Microbiologia Genética Marcadores genéticos Transversal NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inactivation has on gene expression patterns during development and vegetative growth. Gene expression in nla18 mutant cells is altered in the early stages of fruiting body development. Furthermore, nla18 mutant cells are defective for two of the earliest events in development, production of the intracellular starvation signal ppGpp and production of A-signal. Taken together, these results indicate that the developmental program in nla18 mutant cells goes awry very early. Inactivation of nla18 also causes a dramatic decrease in the vegetative growth rate of M. xanthus cells. DNA microarray analysis revealed that the vegetative expression patterns of more than 700 genes are altered in nla18 mutant cells. Genes coding for putative membrane and membrane-associated proteins are among the largest classes of genes whose expression is altered by nla18 inactivation. This result is supported by our findings that the profiles of membrane proteins isolated from vegetative nla18 mutant and wild-type cells are noticeably different. In addition to genes that code for putative membrane proteins, nla18 inactivation affects the expression of many genes that are likely to be important for protein synthesis and gene regulation. Our data are consistent with a model in which Nla18 controls vegetative growth and development by activating the expression of genes involved in gene regulation, translation, and membrane structure. Doctor of Philosophy Doctorado 2019-04-30T18:37:03Z 2019-04-30T18:37:03Z 2006 doctoralThesis Tesis de doctorado http://purl.org/coar/resource_type/c_db06 info:eu-repo/semantics/doctoralThesis https://purl.org/redcol/resource_type/TD http://purl.org/coar/version/c_970fb48d4fbd8a85 http://hdl.handle.net/20.500.12324/35011 55235 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Acceso a texto completo info:eu-repo/semantics/openAccess 131 páginas . application/pdf application/pdf Syracuse University Biology Doctor of Philosophy |
institution |
Corporación Colombiana de Investigación Agropecuaria |
collection |
Repositorio AGROSAVIA |
language |
Inglés |
topic |
Fisiología de la planta nutrición - F61 Protección de plantas aspectos generales - H01 Microbiologia Genética Marcadores genéticos Transversal |
spellingShingle |
Fisiología de la planta nutrición - F61 Protección de plantas aspectos generales - H01 Microbiologia Genética Marcadores genéticos Transversal Ossa Ramírez, Faisury NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus |
description |
NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inactivation has on gene expression patterns during development and vegetative growth. Gene expression in nla18 mutant cells is altered in the early stages of fruiting body development. Furthermore, nla18 mutant cells are defective for two of the earliest events in development, production of the intracellular starvation signal ppGpp and production of A-signal. Taken together, these results indicate that the developmental program in nla18 mutant cells goes awry very early. Inactivation of nla18 also causes a dramatic decrease in the vegetative growth rate of M. xanthus cells. DNA microarray analysis revealed that the vegetative expression patterns of more than 700 genes are altered in nla18 mutant cells. Genes coding for putative membrane and membrane-associated proteins are among the largest classes of genes whose expression is altered by nla18 inactivation. This result is supported by our findings that the profiles of membrane proteins isolated from vegetative nla18 mutant and wild-type cells are noticeably different. In addition to genes that code for putative membrane proteins, nla18 inactivation affects the expression of many genes that are likely to be important for protein synthesis and gene regulation. Our data are consistent with a model in which Nla18 controls vegetative growth and development by activating the expression of genes involved in gene regulation, translation, and membrane structure. |
author2 |
Garza Anthony |
author_facet |
Garza Anthony Ossa Ramírez, Faisury |
format |
doctoralThesis |
author |
Ossa Ramírez, Faisury |
author_sort |
Ossa Ramírez, Faisury |
title |
NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus |
title_short |
NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus |
title_full |
NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus |
title_fullStr |
NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus |
title_full_unstemmed |
NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus |
title_sort |
nla4 and nla18, key regulatory proteins required for normal growth and development of myxococcus xanthus |
publisher |
Syracuse University |
publishDate |
2019 |
url |
http://hdl.handle.net/20.500.12324/35011 |
work_keys_str_mv |
AT ossaramirezfaisury nla4andnla18keyregulatoryproteinsrequiredfornormalgrowthanddevelopmentofmyxococcusxanthus |
_version_ |
1808104932273815552 |