NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus

NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inac...

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Detalles Bibliográficos
Autor principal:	Ossa Ramírez, Faisury
Otros Autores:	Garza Anthony
Formato:	doctoralThesis
Lenguaje:	Inglés
Publicado:	Syracuse University 2019
Materias:	Fisiología de la planta nutrición - F61 Protección de plantas aspectos generales - H01 Microbiologia Genética Marcadores genéticos Transversal
Acceso en línea:	http://hdl.handle.net/20.500.12324/35011

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spelling	RepoAGROSAVIA350112023-02-21T01:02:32Z NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus Ossa Ramírez, Faisury Garza Anthony Fisiología de la planta nutrición - F61 Protección de plantas aspectos generales - H01 Microbiologia Genética Marcadores genéticos Transversal NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inactivation has on gene expression patterns during development and vegetative growth. Gene expression in nla18 mutant cells is altered in the early stages of fruiting body development. Furthermore, nla18 mutant cells are defective for two of the earliest events in development, production of the intracellular starvation signal ppGpp and production of A-signal. Taken together, these results indicate that the developmental program in nla18 mutant cells goes awry very early. Inactivation of nla18 also causes a dramatic decrease in the vegetative growth rate of M. xanthus cells. DNA microarray analysis revealed that the vegetative expression patterns of more than 700 genes are altered in nla18 mutant cells. Genes coding for putative membrane and membrane-associated proteins are among the largest classes of genes whose expression is altered by nla18 inactivation. This result is supported by our findings that the profiles of membrane proteins isolated from vegetative nla18 mutant and wild-type cells are noticeably different. In addition to genes that code for putative membrane proteins, nla18 inactivation affects the expression of many genes that are likely to be important for protein synthesis and gene regulation. Our data are consistent with a model in which Nla18 controls vegetative growth and development by activating the expression of genes involved in gene regulation, translation, and membrane structure. Doctor of Philosophy Doctorado 2019-04-30T18:37:03Z 2019-04-30T18:37:03Z 2006 doctoralThesis Tesis de doctorado http://purl.org/coar/resource_type/c_db06 info:eu-repo/semantics/doctoralThesis https://purl.org/redcol/resource_type/TD http://purl.org/coar/version/c_970fb48d4fbd8a85 http://hdl.handle.net/20.500.12324/35011 55235 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA eng Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ Acceso a texto completo info:eu-repo/semantics/openAccess 131 páginas . application/pdf application/pdf Syracuse University Biology Doctor of Philosophy
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Inglés
topic	Fisiología de la planta nutrición - F61 Protección de plantas aspectos generales - H01 Microbiologia Genética Marcadores genéticos Transversal
spellingShingle	Fisiología de la planta nutrición - F61 Protección de plantas aspectos generales - H01 Microbiologia Genética Marcadores genéticos Transversal Ossa Ramírez, Faisury NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus
description	NtrC-like activators regulate the transcription of a wide variety of adaptive genes in bacteria. Previously, we demonstrated that a mutation in the ntrC-like activator gene nla18 causes defects in fruiting body development in Myxococcus xanthus. In this report, we describe the effect that nla18 inactivation has on gene expression patterns during development and vegetative growth. Gene expression in nla18 mutant cells is altered in the early stages of fruiting body development. Furthermore, nla18 mutant cells are defective for two of the earliest events in development, production of the intracellular starvation signal ppGpp and production of A-signal. Taken together, these results indicate that the developmental program in nla18 mutant cells goes awry very early. Inactivation of nla18 also causes a dramatic decrease in the vegetative growth rate of M. xanthus cells. DNA microarray analysis revealed that the vegetative expression patterns of more than 700 genes are altered in nla18 mutant cells. Genes coding for putative membrane and membrane-associated proteins are among the largest classes of genes whose expression is altered by nla18 inactivation. This result is supported by our findings that the profiles of membrane proteins isolated from vegetative nla18 mutant and wild-type cells are noticeably different. In addition to genes that code for putative membrane proteins, nla18 inactivation affects the expression of many genes that are likely to be important for protein synthesis and gene regulation. Our data are consistent with a model in which Nla18 controls vegetative growth and development by activating the expression of genes involved in gene regulation, translation, and membrane structure.
author2	Garza Anthony
author_facet	Garza Anthony Ossa Ramírez, Faisury
format	doctoralThesis
author	Ossa Ramírez, Faisury
author_sort	Ossa Ramírez, Faisury
title	NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus
title_short	NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus
title_full	NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus
title_fullStr	NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus
title_full_unstemmed	NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus
title_sort	nla4 and nla18, key regulatory proteins required for normal growth and development of myxococcus xanthus
publisher	Syracuse University
publishDate	2019
url	http://hdl.handle.net/20.500.12324/35011
work_keys_str_mv	AT ossaramirezfaisury nla4andnla18keyregulatoryproteinsrequiredfornormalgrowthanddevelopmentofmyxococcusxanthus
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NLA4 and NLA18, key regulatory proteins required for normal growth and development of Myxococcus Xanthus

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