Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador

Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador

One of the most damaging diseases in cultivation of avocado (Persea americana Mill.) is root rot associated with Phytophthora cinnamomi Rands. This disease causes progressive wilt and even death of the tree. The objective of this study was to identify the presence of P. cinnamomi in two productive a...

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Autores principales: Toapanta Gallegos, Diana Elizabeth, Morillo Velastegui, Luis Eduardo, Viera Arroyo, William Fernando
Formato: article
Lenguaje:Español
Publicado: ‎‎Corporación colombiana de investigación agropecuaria - AGROSAVIA 2018
Materias:
Frutales
Acceso en línea:http://revista.corpoica.org.co/index.php/revista/article/view/628
http://hdl.handle.net/20.500.12324/34019
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institution Corporación Colombiana de Investigación Agropecuaria
collection Repositorio AGROSAVIA
language Español
topic Frutales
spellingShingle Frutales
Toapanta Gallegos, Diana Elizabeth
Morillo Velastegui, Luis Eduardo
Viera Arroyo, William Fernando
Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador
description One of the most damaging diseases in cultivation of avocado (Persea americana Mill.) is root rot associated with Phytophthora cinnamomi Rands. This disease causes progressive wilt and even death of the tree. The objective of this study was to identify the presence of P. cinnamomi in two productive areas of avocado in Ecuador using the molecular technique PCR-RFLP. Tree root samples were obtained with root rot symptoms in the production areas, from which 10 isolates were morphologically identified with Phytophthora spp. infection. To distinguish among the various Phytophthora species, a molecular analysis was performed using molecular markers in the ITSregion of the ribosomal DNA (rDNA). The ITSdigestion fragment obtained by PCR with the Ta qI enzyme confirmed the presence of Phytophthora cinnamomi in the isolated samples, and its association with root rot in the sampled production areas. 
format article
author Toapanta Gallegos, Diana Elizabeth
Morillo Velastegui, Luis Eduardo
Viera Arroyo, William Fernando
author_facet Toapanta Gallegos, Diana Elizabeth
Morillo Velastegui, Luis Eduardo
Viera Arroyo, William Fernando
author_sort Toapanta Gallegos, Diana Elizabeth
title Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador
title_short Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador
title_full Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador
title_fullStr Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador
title_full_unstemmed Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador
title_sort molecular diagnosis of phytophthora cinnamomi associated with root rot in avocado producing areas of ecuador
publisher ‎‎Corporación colombiana de investigación agropecuaria - AGROSAVIA
publishDate 2018
url http://revista.corpoica.org.co/index.php/revista/article/view/628
http://hdl.handle.net/20.500.12324/34019
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spelling RepoAGROSAVIA340192023-05-05T03:00:48Z Molecular diagnosis of Phytophthora cinnamomi associated with root rot in avocado producing areas of Ecuador Diagnóstico molecular de Phytophthora cinnamomi asociado a la pudrición radicular en zonas productoras de aguacate en Ecuador Diagnóstico molecular de Phytophthora cinnamomi associado com a podridão radicular em áreas produtoras de abacate no Equador Toapanta Gallegos, Diana Elizabeth Morillo Velastegui, Luis Eduardo Viera Arroyo, William Fernando Frutales One of the most damaging diseases in cultivation of avocado (Persea americana Mill.) is root rot associated with Phytophthora cinnamomi Rands. This disease causes progressive wilt and even death of the tree. The objective of this study was to identify the presence of P. cinnamomi in two productive areas of avocado in Ecuador using the molecular technique PCR-RFLP. Tree root samples were obtained with root rot symptoms in the production areas, from which 10 isolates were morphologically identified with Phytophthora spp. infection. To distinguish among the various Phytophthora species, a molecular analysis was performed using molecular markers in the ITSregion of the ribosomal DNA (rDNA). The ITSdigestion fragment obtained by PCR with the Ta qI enzyme confirmed the presence of Phytophthora cinnamomi in the isolated samples, and its association with root rot in the sampled production areas.  Phytophthora cinnamomi Rands está asociado con la pudrición radicular, una de las enfermedades más importantes en el cultivo de aguacate (Persea americana Mill.) debido a que ocasiona marchitez progresiva hasta provocar la muerte del árbol. El objetivo de este estudio fue identificar molecularmente la presencia de P. cinnamomi con la técnica PCR-RFLP, en plantaciones de aguacate en dos zonas productoras del Ecuador. Con este fin, se obtuvieron muestras de raíces de árboles con sintomatología de pudrición radicular en las zonas Palabras clave: aislamiento, Ecuador, Persea americana, Phytophthora cinnamomi de producción. A partir de estas se obtuvieron diez aislamientos donde se identificó morfológicamente a Phytophthora sp. Para la distinción de especies de Phytophthora se realizó un análisis molecular con marcadores en la región ITS de ADN ribosomal (ADNr). La digestión del fragmento ITS obtenido por PCR con la enzima TaqI permitió confirmar la presencia de P. cinnamomi en los aislamientos y, por lo tanto, asociar su presencia con la pudrición radicular en las zonas de producción muestreadas. Phytophthora cinnamomi Rands está associado com a podridão radicular, uma das doenças mais importantes no cultivo de abacate (Persea americanaMill.) devido a que ocasiona murchidão progressiva até provocar a morte da árvore. O objetivo deste estudo foi identificar molecularmente a presença de P. cinnamomi com a técnica PCR-RFLP, em plantações de abacate em duas áreas produtoras do Equador. Com esse propósito, obtiveram-se amostras de raízes de árvores com sintomatologia de podridão radicular nas áreas de produção. A partir destas, obtiveram-se dez isolamentos nos quais se identificou morfologicamente a Phytophthora sp. Para a distinção de espécies de Phytophthora, foi realizada uma análise molecular com marcadores na região ITS de DNAribossômico (DNAr). A digestão do fragmento ITS obtido por PCR com a enzima Ta qI permitiu confirmar a presença de P. cinnamomi nos isolamentos e, portanto, associar sua presença com a podridão radicular nas áreas de produção das amostras. Aguacate-Persea americana 2018-11-06T20:41:44Z 2018-11-06T20:41:44Z 2017 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 http://revista.corpoica.org.co/index.php/revista/article/view/628 10.21930/rcta.vol18_num2_art:628 http://hdl.handle.net/20.500.12324/34019 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA spa http://revista.corpoica.org.co/index.php/revista/article/view/628/444 Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ info:eu-repo/semantics/openAccess application/pdf application/pdf Colombia ‎‎Corporación colombiana de investigación agropecuaria - AGROSAVIA Revista Ciencia y Tecnología Agropecuaria; Vol 18 No 2 (2017); 285-294

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