Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States
This study discusses the diagnosis and detection of the new citrus leprosis virus cytoplasmic type 2 (CiLV-C2) in samples collected in Meta and Casanare States, using molecular techniques performed RT-PCR on plant tissue and mite vectors Brevipalpus phoenicis (Geijskes). RT-PCR tests were carry out...
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Corporación colombiana de investigación agropecuaria - AGROSAVIA
2018
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| Acceso en línea: | http://revista.corpoica.org.co/index.php/revista/article/view/360 http://hdl.handle.net/20.500.12324/33930 |
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RepoAGROSAVIA339302024-02-05T21:41:14Z Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States Detección del virus de la leprosis de los cítricos tipo 2 citoplasmático (CiLV-C2) en los departamentos de Meta y Casanare León, Guillermo Roy, Avijit Choudhary, Nandlal Brlansky, Ronald Poncirus trifoliata Virus de las plantas Diagnóstico Frutales http://aims.fao.org/aos/agrovoc/c_6103 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_2238 This study discusses the diagnosis and detection of the new citrus leprosis virus cytoplasmic type 2 (CiLV-C2) in samples collected in Meta and Casanare States, using molecular techniques performed RT-PCR on plant tissue and mite vectors Brevipalpus phoenicis (Geijskes). RT-PCR tests were carry out using specific primers MP and CPG. The use of CPG primers allows the detection of CiLV-C2, in tissue plant samples with leprosis symptoms on fruits and leaves of Valencia orange (Citrus sinensis L.) and Swinglea gluinosa Merr. leaves, which confirms the presence of this virus in Colombia. The MP primers did not detect the CiLV-C2 in any of performed tests. The RT-PCR technique allows detection of CiLV-C2 in samples of B. phoenicis mite vectors, with the specific CPG primers. Molecular detection of CiLV-C2 by RT-PCR technique is a fundamental tool for the virus diagnosis in Colombia, and should be used in diagnosis and prevention programs of citrus leprosis in the country. Este estudio trata del diagnóstico y detección del nuevo virus de la leprosis de los cítricos tipo 2 citoplasmático (CiLV-C2) en muestras colectadas en los departamentos del Meta y Casanare, mediante técnicas moleculares RT-PCR realizadas sobre tejido vegetal y ácaros vectores Brevipalpus phoenicis (Geijskes). Para las pruebas de detección RT-PCR se utilizaron los iniciadores específicos MP y CPG. Con los iniciadores CPG, se logró la detección del CiLV-C2, en muestras de tejido vegetal con síntomas de leprosis en frutos y hojas de naranja valencia (Citrus sinensis L.) y hojas de Swinglea gluinosa Merr., lo cual confirma la presencia de este virus en Colombia. Los iniciadores MP no detectaron el virus CiLV-C2 en ninguna de las pruebas realizadas. La técnica RT-PCR permitió detectar el virus CiLV-C2 en muestras de ácaros vectores B. phoenicis, cuando se utilizaron los iniciadores específicos CPG. La detección molecular del CiLV-C2, mediante la técnica RT-PCR, se constituye en un instrumento fundamental para el diagnóstico del virus en Colombia y debe ser usada en los programas de detección y prevención de la leprosis de los cítricos en el país. 2018-11-06T20:41:33Z 2018-11-06T20:41:33Z 2014 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 http://revista.corpoica.org.co/index.php/revista/article/view/360 10.21930/rcta.vol15_num2_art:360 http://hdl.handle.net/20.500.12324/33930 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA spa http://revista.corpoica.org.co/index.php/revista/article/view/360/350 Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ info:eu-repo/semantics/openAccess application/pdf application/pdf Colombia Corporación colombiana de investigación agropecuaria - AGROSAVIA Revista Ciencia y Tecnología Agropecuaria; Vol 15 No 2 (2014); p. 207-217 |
| institution |
Corporación Colombiana de Investigación Agropecuaria |
| collection |
Repositorio AGROSAVIA |
| language |
Español |
| topic |
Poncirus trifoliata Virus de las plantas Diagnóstico Frutales http://aims.fao.org/aos/agrovoc/c_6103 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_2238 |
| spellingShingle |
Poncirus trifoliata Virus de las plantas Diagnóstico Frutales http://aims.fao.org/aos/agrovoc/c_6103 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_2238 León, Guillermo Roy, Avijit Choudhary, Nandlal Brlansky, Ronald Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States |
| description |
This study discusses the diagnosis and detection of the new citrus leprosis virus cytoplasmic type 2 (CiLV-C2) in samples collected in Meta and Casanare States, using molecular techniques performed RT-PCR on plant tissue and mite vectors Brevipalpus phoenicis (Geijskes). RT-PCR tests were carry out using specific primers MP and CPG. The use of CPG primers allows the detection of CiLV-C2, in tissue plant samples with leprosis symptoms on fruits and leaves of Valencia orange (Citrus sinensis L.) and Swinglea gluinosa Merr. leaves, which confirms the presence of this virus in Colombia. The MP primers did not detect the CiLV-C2 in any of performed tests. The RT-PCR technique allows detection of CiLV-C2 in samples of B. phoenicis mite vectors, with the specific CPG primers. Molecular detection of CiLV-C2 by RT-PCR technique is a fundamental tool for the virus diagnosis in Colombia, and should be used in diagnosis and prevention programs of citrus leprosis in the country. |
| format |
article |
| author |
León, Guillermo Roy, Avijit Choudhary, Nandlal Brlansky, Ronald |
| author_facet |
León, Guillermo Roy, Avijit Choudhary, Nandlal Brlansky, Ronald |
| author_sort |
León, Guillermo |
| title |
Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States |
| title_short |
Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States |
| title_full |
Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States |
| title_fullStr |
Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States |
| title_full_unstemmed |
Citrus leprosis virus cytoplasmic type 2 (CiLV-C2) detection in Meta and Casanare States |
| title_sort |
citrus leprosis virus cytoplasmic type 2 (cilv-c2) detection in meta and casanare states |
| publisher |
Corporación colombiana de investigación agropecuaria - AGROSAVIA |
| publishDate |
2018 |
| url |
http://revista.corpoica.org.co/index.php/revista/article/view/360 http://hdl.handle.net/20.500.12324/33930 |
| work_keys_str_mv |
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