Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)

The knowledge of genetic diversity in Amazonian species is of great importance for domestication and breeding purposes. A great difficulty with non-domesticated species such as “camu-camu” is the lack of information about their genetic variability. Due to the economic potential of camu-camu, a fruit...

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Main Authors:	Rojas, Salvador, Clement Ch., Yuyama K., Nagao, Eduardo Ossamu
Format:	article
Language:	Español
Published:	‎‎Corporación colombiana de investigación agropecuaria - AGROSAVIA 2018
Subjects:	Propagación de plantas - F02 Myrciaria cauliflora Germoplasma Variación genética Microsatélites Marcadores genéticos Fitomejoramiento Transversal
Online Access:	http://hdl.handle.net/20.500.12324/33877

id	RepoAGROSAVIA33877
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spelling	RepoAGROSAVIA338772024-02-06T03:00:14Z Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR) Diversidade genética em acessos do banco de germoplasma de camu-camu (Myrciaria dúbia [H.B.K.] McVaugh) do INPA usando marcadores microssatélites (EST-SSR). Rojas, Salvador Clement Ch., Yuyama K. Nagao, Eduardo Ossamu Propagación de plantas - F02 Myrciaria cauliflora Germoplasma Variación genética Microsatélites Marcadores genéticos Fitomejoramiento Transversal The knowledge of genetic diversity in Amazonian species is of great importance for domestication and breeding purposes. A great difficulty with non-domesticated species such as “camu-camu” is the lack of information about their genetic variability. Due to the economic potential of camu-camu, a fruit with a high level of vitamin C production, the aim of this study was to estimate the genetic diversity using the molecular markers EST-SSR, to study the genetic variability of 139 accessions from 17 “camu-camu” materiales from different rivers in the Brazilian's Amazon region, preserved at the INPA (Instituto Nacional de Pesquisas da Amazônia Brasilera) Active Germoplasm Bank (BAG) of “camu-camu” in Manaus. Eight of the EST-SSR polimorfic loci used had 102 alleles detected with an average of 12.87 alleles per locus. The results show high levels of diversity for all loci with an average expected heterocygocity (He) of 0.797 and a total heterocygocity (Ht) value of 0.502. Populations had high inbreeding values, suggesting a heterocigotes deficiency as observed by the heterocigocity (Ho), perhaps as result of the great distance and isolation among populations, which limits gene flow and favors inbreeding. A high genetic differentiation value (FST) of 0.21, indicates high variability among populations. Measures based on the alleles frequency, showed a larger variability within populations (80.3%) than among populations (19.7%). Genetic distances between populations showed high differences within accessions coming from Rondonia (Jaru) and those from Amazonas (and Pirarucu Tarumã) and Roraima (Urubu). The dendogram made by the UPGMA method, showed two major groups and five subgroups related geographically. Results proved EST-SSR marker's utility in genetic diversity studies among BAG of camu-camu. These results will be useful in planning new collections, germplasm conservation and population variability analysis, as well as directional crossover using divergent genotypes; which will contribute to camu-camu breeding in Amazonian countries. 2018-11-06T20:41:26Z 2018-11-06T20:41:26Z 2011 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 10.21930/rcta.vol12_num1_art:215 http://hdl.handle.net/20.500.12324/33877 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA spa Attribution-NonCommercial-ShareAlike 4.0 International http://creativecommons.org/licenses/by-nc-sa/4.0/ info:eu-repo/semantics/openAccess application/pdf application/pdf Amazonas Colombia ‎‎Corporación colombiana de investigación agropecuaria - AGROSAVIA Revista Ciencia y Tecnología Agropecuaria; Vol 12 No 1 (2011); p. 51-64
institution	Corporación Colombiana de Investigación Agropecuaria
collection	Repositorio AGROSAVIA
language	Español
topic	Propagación de plantas - F02 Myrciaria cauliflora Germoplasma Variación genética Microsatélites Marcadores genéticos Fitomejoramiento Transversal
spellingShingle	Propagación de plantas - F02 Myrciaria cauliflora Germoplasma Variación genética Microsatélites Marcadores genéticos Fitomejoramiento Transversal Rojas, Salvador Clement Ch., Yuyama K. Nagao, Eduardo Ossamu Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)
description	The knowledge of genetic diversity in Amazonian species is of great importance for domestication and breeding purposes. A great difficulty with non-domesticated species such as “camu-camu” is the lack of information about their genetic variability. Due to the economic potential of camu-camu, a fruit with a high level of vitamin C production, the aim of this study was to estimate the genetic diversity using the molecular markers EST-SSR, to study the genetic variability of 139 accessions from 17 “camu-camu” materiales from different rivers in the Brazilian's Amazon region, preserved at the INPA (Instituto Nacional de Pesquisas da Amazônia Brasilera) Active Germoplasm Bank (BAG) of “camu-camu” in Manaus. Eight of the EST-SSR polimorfic loci used had 102 alleles detected with an average of 12.87 alleles per locus. The results show high levels of diversity for all loci with an average expected heterocygocity (He) of 0.797 and a total heterocygocity (Ht) value of 0.502. Populations had high inbreeding values, suggesting a heterocigotes deficiency as observed by the heterocigocity (Ho), perhaps as result of the great distance and isolation among populations, which limits gene flow and favors inbreeding. A high genetic differentiation value (FST) of 0.21, indicates high variability among populations. Measures based on the alleles frequency, showed a larger variability within populations (80.3%) than among populations (19.7%). Genetic distances between populations showed high differences within accessions coming from Rondonia (Jaru) and those from Amazonas (and Pirarucu Tarumã) and Roraima (Urubu). The dendogram made by the UPGMA method, showed two major groups and five subgroups related geographically. Results proved EST-SSR marker's utility in genetic diversity studies among BAG of camu-camu. These results will be useful in planning new collections, germplasm conservation and population variability analysis, as well as directional crossover using divergent genotypes; which will contribute to camu-camu breeding in Amazonian countries.
format	article
author	Rojas, Salvador Clement Ch., Yuyama K. Nagao, Eduardo Ossamu
author_facet	Rojas, Salvador Clement Ch., Yuyama K. Nagao, Eduardo Ossamu
author_sort	Rojas, Salvador
title	Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)
title_short	Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)
title_full	Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)
title_fullStr	Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)
title_full_unstemmed	Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)
title_sort	genetic diversity of the inpa germoplasm bank of camu-camu (myrciaria dubia [h.b.k.] mcvaugh) using microsatellites markers (est-ssr)
publisher	‎‎Corporación colombiana de investigación agropecuaria - AGROSAVIA
publishDate	2018
url	http://hdl.handle.net/20.500.12324/33877
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Genetic diversity of the INPA germoplasm bank of camu-camu (Myrciaria dubia [H.B.K.] McVaugh) using microsatellites markers (EST-SSR)

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