Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain
The 3¢ untranslated region (3¢ UTR) of the bovine natural resistanceassociated macrophage gene (NRAMP1 or SLC11A1) was genotyped in Colombian Creole Blanco Orejinegro (BON) (Bos taurus) (n = 140) and Zebu Brahman (Bos indicus) (Z) (n = 20) cattle and their crosses (BON · Zebu Brahman [B · Z] [n =...
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Blackwell Verlag
2018
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| Acceso en línea: | http://hdl.handle.net/20.500.12324/1011 |
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RepoAGROSAVIA10112023-11-09T13:07:22Z Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain Martínez, R. Toro R., R. Montoya, F. Burbano, M. Tobón, J. Gallego, J. Dunner, J. Cañón, J. Enfermedades de los animales - L73 Ganado bovino Brucella abortus Enfermedades de los animales ADN Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_23901 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_2347 The 3¢ untranslated region (3¢ UTR) of the bovine natural resistanceassociated macrophage gene (NRAMP1 or SLC11A1) was genotyped in Colombian Creole Blanco Orejinegro (BON) (Bos taurus) (n = 140) and Zebu Brahman (Bos indicus) (Z) (n = 20) cattle and their crosses (BON · Zebu Brahman [B · Z] [n = 10]; Zebu Brahman · BON [Z · B] [n = 10]), and in animals from a Holstein · BON (H · B) (n = 10) cross. Direct sequencing and single-strand conformation polymorphism analysis (SSCP) helped in detecting the polymorphic behaviour. The association between resistance to brucellosis infection and SSCP genotype was evaluated using a macrophage in vitro killing assay employing a virulent Brucella abortus strain. The 3¢ UTR (GT) repeated polymorphism was gentoyped and its association with resistance to brucellosis was evaluated. When all breeds were grouped, a high frequency in the homozygote GT12 (AA genotype) (0.823) and a very low frequency in the homozygote GT10 (BB genotype) (0.047) were detected. The BON (0.963), Z · B (0.60) and H · B (1.00) cattle showed high GT12 allele frequencies, unlike that seen for the B · Z and Zebu cattle (0.3002 and 0.218, respectively). The GT10 allele was only found in the Zebu cattle (0.391). A significant association (p < 0.001) was found between the B. abortus macrophage in vitro killing assay phenotypes and the bovine SLC11A1 3¢ UTR genotypes, which suggests that the A allele may be associated with resistance. Because only nine animals had the BB genotype, the results require some confirmation in more extensive populations. Ganadería bovina 2018-09-08T17:28:58Z 2018-09-08T17:28:58Z 2008 article Artículo científico http://purl.org/coar/resource_type/c_2df8fbb1 info:eu-repo/semantics/article https://purl.org/redcol/resource_type/ART http://purl.org/coar/version/c_970fb48d4fbd8a85 http://hdl.handle.net/20.500.12324/1011 64545 reponame:Biblioteca Digital Agropecuaria de Colombia repourl:https://repository.agrosavia.co instname:Corporación colombiana de investigación agropecuaria AGROSAVIA spa Journal of Animal Breeding and Genetics 125 1 9 Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/ Acceso a texto completo info:eu-repo/semantics/openAccess application/pdf application/pdf Colombia Blackwell Verlag Berlin Journal of Animal Breeding and Genetics; v.125; 2008 |
| institution |
Corporación Colombiana de Investigación Agropecuaria |
| collection |
Repositorio AGROSAVIA |
| language |
Español |
| topic |
Enfermedades de los animales - L73 Ganado bovino Brucella abortus Enfermedades de los animales ADN Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_23901 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_2347 |
| spellingShingle |
Enfermedades de los animales - L73 Ganado bovino Brucella abortus Enfermedades de los animales ADN Ganadería y especies menores http://aims.fao.org/aos/agrovoc/c_1391 http://aims.fao.org/aos/agrovoc/c_23901 http://aims.fao.org/aos/agrovoc/c_426 http://aims.fao.org/aos/agrovoc/c_2347 Martínez, R. Toro R., R. Montoya, F. Burbano, M. Tobón, J. Gallego, J. Dunner, J. Cañón, J. Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain |
| description |
The 3¢ untranslated region (3¢ UTR) of the bovine natural resistanceassociated macrophage gene (NRAMP1 or SLC11A1) was genotyped in
Colombian Creole Blanco Orejinegro (BON) (Bos taurus) (n = 140) and
Zebu Brahman (Bos indicus) (Z) (n = 20) cattle and their crosses
(BON · Zebu Brahman [B · Z] [n = 10]; Zebu Brahman · BON [Z · B]
[n = 10]), and in animals from a Holstein · BON (H · B) (n = 10) cross.
Direct sequencing and single-strand conformation polymorphism analysis (SSCP) helped in detecting the polymorphic behaviour. The association between resistance to brucellosis infection and SSCP genotype was
evaluated using a macrophage in vitro killing assay employing a virulent
Brucella abortus strain. The 3¢ UTR (GT) repeated polymorphism was gentoyped and its association with resistance to brucellosis was evaluated.
When all breeds were grouped, a high frequency in the homozygote
GT12 (AA genotype) (0.823) and a very low frequency in the homozygote GT10 (BB genotype) (0.047) were detected. The BON (0.963), Z · B
(0.60) and H · B (1.00) cattle showed high GT12 allele frequencies,
unlike that seen for the B · Z and Zebu cattle (0.3002 and 0.218,
respectively). The GT10 allele was only found in the Zebu cattle (0.391).
A significant association (p < 0.001) was found between the B. abortus
macrophage in vitro killing assay phenotypes and the bovine SLC11A1 3¢
UTR genotypes, which suggests that the A allele may be associated with
resistance. Because only nine animals had the BB genotype, the results
require some confirmation in more extensive populations. |
| format |
article |
| author |
Martínez, R. Toro R., R. Montoya, F. Burbano, M. Tobón, J. Gallego, J. Dunner, J. Cañón, J. |
| author_facet |
Martínez, R. Toro R., R. Montoya, F. Burbano, M. Tobón, J. Gallego, J. Dunner, J. Cañón, J. |
| author_sort |
Martínez, R. |
| title |
Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain |
| title_short |
Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain |
| title_full |
Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain |
| title_fullStr |
Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain |
| title_full_unstemmed |
Bovine SLC11A1 3´ UTR SSCP genotype evaluated by a macrophage in vitro killing assay employing a Brucella abortus strain |
| title_sort |
bovine slc11a1 3´ utr sscp genotype evaluated by a macrophage in vitro killing assay employing a brucella abortus strain |
| publisher |
Blackwell Verlag |
| publishDate |
2018 |
| url |
http://hdl.handle.net/20.500.12324/1011 |
| work_keys_str_mv |
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