Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management
Essential oils (EOs) are a promising alternative to conventional pesticides, but some challenges like high volatility, poor water solubility, and rapid degradation limit their use in Integrated Pest Management (IPM). To overcome these limitations, this study aimed to develop garlic, eucalyptus, an...
| Autores principales: | , , , , , , , , |
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| Formato: | publishedVersion |
| Lenguaje: | Inglés |
| Publicado: |
Elsevier
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9097 https://www.sciencedirect.com/science/article/pii/S0048357525002822?via%3Dihub |
| _version_ | 1855032911276802048 |
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| author | Modafferi, Antonino Urbaneja, Alberto Aure, C.M. Giunti, G. Martínez-Sañudo, I. Ienco, A. Campolo, O. Palmeri, V. Pérez-Hedo, M. |
| author_browse | Aure, C.M. Campolo, O. Giunti, G. Ienco, A. Martínez-Sañudo, I. Modafferi, Antonino Palmeri, V. Pérez-Hedo, M. Urbaneja, Alberto |
| author_facet | Modafferi, Antonino Urbaneja, Alberto Aure, C.M. Giunti, G. Martínez-Sañudo, I. Ienco, A. Campolo, O. Palmeri, V. Pérez-Hedo, M. |
| author_sort | Modafferi, Antonino |
| collection | ReDivia |
| description | Essential oils (EOs) are a promising alternative to conventional pesticides, but some challenges like high volatility,
poor water solubility, and rapid degradation limit their use in Integrated Pest Management (IPM). To
overcome these limitations, this study aimed to develop garlic, eucalyptus, and clove EO-based nano-emulsions
(EO-NEs) in a bait treatment format through the high-pressure microfluidization technique and investigated the
biological activities against Ceratitis capitata. In addition, the adverse effects of the most promising nanoemulsion
were evaluated towards a non-target parasitoid Anagaspis daci. The garlic nano-emulsion (garlic-NE)
with estimated LD50 and LD90 of 0.96 and 2.18 % of EO showed high insecticidal activity against C. capitata.
Instead, eucalyptus and clove nano-emulsions exibhited low and mediuam mortality rates respectively. However,
garlic-NE exhibited high toxicity (100 % mortality) towards the non-target parasitoid A. daci. We analyzed the
expression of 14 genes involved in detoxification pathways to investigate the mechanisms underlying garlic-NE
toxicity. Our results showed that garlic-NE triggered a strong metabolic detoxification response, leading to the
upregulation of cytochrome P450 monooxygenases (CYPs), glutathione S-transferases (GSTs), and alcohol dehydrogenase
(Adh). Furthermore, garlic-NE induced significant shifts in the gut microbiota composition of
C. capitata 72 h after the treatment, disrupting its microbial homeostasis. This dual effect—metabolic stress
combined with microbiota disruption—suggests that garlic-NE exerts its insecticidal action through a multifaceted
mode of action rather than direct neurotoxicity, potentially reducing the risk of resistance development.
Overall, this study highlights the potential of EO-based nano-formulations as alternatives to conventional
pesticides. |
| format | publishedVersion |
| id | ReDivia9097 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia90972025-12-18T19:35:36Z Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management Modafferi, Antonino Urbaneja, Alberto Aure, C.M. Giunti, G. Martínez-Sañudo, I. Ienco, A. Campolo, O. Palmeri, V. Pérez-Hedo, M. Non-target organism Detoxification system H10 Pests of plants Fruit flies Biopesticides Essential oils (EOs) are a promising alternative to conventional pesticides, but some challenges like high volatility, poor water solubility, and rapid degradation limit their use in Integrated Pest Management (IPM). To overcome these limitations, this study aimed to develop garlic, eucalyptus, and clove EO-based nano-emulsions (EO-NEs) in a bait treatment format through the high-pressure microfluidization technique and investigated the biological activities against Ceratitis capitata. In addition, the adverse effects of the most promising nanoemulsion were evaluated towards a non-target parasitoid Anagaspis daci. The garlic nano-emulsion (garlic-NE) with estimated LD50 and LD90 of 0.96 and 2.18 % of EO showed high insecticidal activity against C. capitata. Instead, eucalyptus and clove nano-emulsions exibhited low and mediuam mortality rates respectively. However, garlic-NE exhibited high toxicity (100 % mortality) towards the non-target parasitoid A. daci. We analyzed the expression of 14 genes involved in detoxification pathways to investigate the mechanisms underlying garlic-NE toxicity. Our results showed that garlic-NE triggered a strong metabolic detoxification response, leading to the upregulation of cytochrome P450 monooxygenases (CYPs), glutathione S-transferases (GSTs), and alcohol dehydrogenase (Adh). Furthermore, garlic-NE induced significant shifts in the gut microbiota composition of C. capitata 72 h after the treatment, disrupting its microbial homeostasis. This dual effect—metabolic stress combined with microbiota disruption—suggests that garlic-NE exerts its insecticidal action through a multifaceted mode of action rather than direct neurotoxicity, potentially reducing the risk of resistance development. Overall, this study highlights the potential of EO-based nano-formulations as alternatives to conventional pesticides. 2025-12-18T19:34:48Z 2025-12-18T19:34:48Z 2025 publishedVersion Modafferi, A., Urbaneja, A., Aure, C. M., Giunti, G., Martinez Sanudo, I., Ienco, A., … Pérez-Hedo, M. (2025). Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management. Pesticide Biochemistry and Physiology, 214, 106569. https://hdl.handle.net/20.500.11939/9097 10.1016/j.pestbp.2025.106569 https://www.sciencedirect.com/science/article/pii/S0048357525002822?via%3Dihub en PID2020-113234RR-I00 IVIA-52202B from Instituto Valenciano de Investigaciones Agrarias (IVIA) openAccess Elsevier electronico |
| spellingShingle | Non-target organism Detoxification system H10 Pests of plants Fruit flies Biopesticides Modafferi, Antonino Urbaneja, Alberto Aure, C.M. Giunti, G. Martínez-Sañudo, I. Ienco, A. Campolo, O. Palmeri, V. Pérez-Hedo, M. Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management |
| title | Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management |
| title_full | Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management |
| title_fullStr | Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management |
| title_full_unstemmed | Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management |
| title_short | Metabolic and microbial responses of Ceratitis capitata to essential oil-based nano-emulsions: Implications for pest management |
| title_sort | metabolic and microbial responses of ceratitis capitata to essential oil based nano emulsions implications for pest management |
| topic | Non-target organism Detoxification system H10 Pests of plants Fruit flies Biopesticides |
| url | https://hdl.handle.net/20.500.11939/9097 https://www.sciencedirect.com/science/article/pii/S0048357525002822?via%3Dihub |
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