High Resolution Melting DNA analysis for the traceability of plants and juices of blond and pigmented sweet orange
The economic relevance of sweet orange [Citrus sinensis (L.) Osbeck] raised the interest for the set-up of robust and scalable methods to assess the origin of plants and derived products. Molecular markers, such as singlenucleotide polymorphisms (SNPs), represent a robust tool for the univocal ide...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
ELSEVIER
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9077 https://www.sciencedirect.com/science/article/pii/S2666154325001267?via%3Dihub |
| Sumario: | The economic relevance of sweet orange [Citrus sinensis (L.) Osbeck] raised the interest for the set-up of robust
and scalable methods to assess the origin of plants and derived products. Molecular markers, such as singlenucleotide
polymorphisms (SNPs), represent a robust tool for the univocal identification of the genetic origin
of a specific cultivar (or group of accessions) with relevant application for traceability.
In this work, the whole-genome sequencing of 29 accessions representing the four varietal groups of sweet
orange (i.e.: Common, Navel, Acidless and Pigmented) allowed the in silico detection of unique SNPs to
discriminate each group (group-specific SNPs) and ten pigmented orange accessions (genotype-specific SNPs).
A subset of the SNPs detected in silico was then validated through a high-resolution melting assay (HRM) on
additional 81 genotypes held in three ex-situ collections in Italy and Spain and on several matrices: leaves, handsqueezed
and commercial fresh-squeezed juices, processed orange juices, and beverages. The HRM assay allowed
the identification of 13 group-specific and 30 genotype-specific SNPs showing consistent results across the whole
germplasm and the different food matrices tested. The HRM assay proved its efficacy also for the identification of
juice blending with other sweet orange varieties (with a detection limit of 5 %). The identified unique SNPs
represent a valuable tool to trace the varietal correspondence of plants and fruit-derived products. Their
implementation with a scalable and robust HRM assay could be readily employed by growers and the food
processing industry to ensure traceability along the chain. |
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