The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C
Artificial insemination in goats commonly relies on refrigerated semen doses, yet the optimal energetic substrate to support sperm metabolism remains unclear. This study aimed to evaluate the effects of different energetic substrates on goat buck sperm metabolism and motility when refrigerated at 17...
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| Format: | Artículo |
| Language: | Inglés |
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MPDI
2025
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| Online Access: | https://hdl.handle.net/20.500.11939/9076 https://www.mdpi.com/2079-7737/14/7/782 |
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| author | Gacem, Sabrina Mocé, Eva Gozalbo, Carmen Albuixech-Benetó, Marta ESTEVE, INÉS CAROLINA Martínez-Talaván, Amparo Silvestre, Miguel Ángel |
| author_browse | Albuixech-Benetó, Marta ESTEVE, INÉS CAROLINA Gacem, Sabrina Gozalbo, Carmen Martínez-Talaván, Amparo Mocé, Eva Silvestre, Miguel Ángel |
| author_facet | Gacem, Sabrina Mocé, Eva Gozalbo, Carmen Albuixech-Benetó, Marta ESTEVE, INÉS CAROLINA Martínez-Talaván, Amparo Silvestre, Miguel Ángel |
| author_sort | Gacem, Sabrina |
| collection | ReDivia |
| description | Artificial insemination in goats commonly relies on refrigerated semen doses, yet the optimal energetic substrate to support sperm metabolism remains unclear. This study aimed to evaluate the effects of different energetic substrates on goat buck sperm metabolism and motility when refrigerated at 17 °C. Semen from six Murciano-Granadina male goats were collected and diluted in PBS supplemented with 35 mM of either glucose, fructose, pyruvate, or lactate in the first experiment. In the second experiment, the effects of varying concentrations of pyruvate and/or glucose, NaCl supplementation, and the osmolarity on sperm quality parameters were assessed. Semen was stored at 17 °C for 48 h and evaluated for motility using the CASA system, as well as for viability, mitochondrial membrane potential, and mitochondrial ROS by flow cytometry. The results show that pyruvate and lactate extenders outperformed the others, preserving higher total motility, progressivity, and viability of spermatozoa over 48 h, even at a concentration lower than 35 mM, as in the case of pyruvate. In contrast, glucose had a detrimental effect on sperm quality, reducing viability and healthy population rates while increasing motility, especially at higher concentrations. NaCl supplementation and osmolarity had no significant effect on any of the sperm quality parameters. In conclusion, pyruvate maintains a higher quality and motility of sperm stored at 17 °C in PBS in comparison with a glucose-supplemented extender. |
| format | Artículo |
| id | ReDivia9076 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MPDI |
| publisherStr | MPDI |
| record_format | dspace |
| spelling | ReDivia90762025-07-03T06:34:36Z The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C Gacem, Sabrina Mocé, Eva Gozalbo, Carmen Albuixech-Benetó, Marta ESTEVE, INÉS CAROLINA Martínez-Talaván, Amparo Silvestre, Miguel Ángel L53 Animal physiology - Reproduction Pyruvates Lactates Fructose Glucose Sperm Goats Artificial insemination in goats commonly relies on refrigerated semen doses, yet the optimal energetic substrate to support sperm metabolism remains unclear. This study aimed to evaluate the effects of different energetic substrates on goat buck sperm metabolism and motility when refrigerated at 17 °C. Semen from six Murciano-Granadina male goats were collected and diluted in PBS supplemented with 35 mM of either glucose, fructose, pyruvate, or lactate in the first experiment. In the second experiment, the effects of varying concentrations of pyruvate and/or glucose, NaCl supplementation, and the osmolarity on sperm quality parameters were assessed. Semen was stored at 17 °C for 48 h and evaluated for motility using the CASA system, as well as for viability, mitochondrial membrane potential, and mitochondrial ROS by flow cytometry. The results show that pyruvate and lactate extenders outperformed the others, preserving higher total motility, progressivity, and viability of spermatozoa over 48 h, even at a concentration lower than 35 mM, as in the case of pyruvate. In contrast, glucose had a detrimental effect on sperm quality, reducing viability and healthy population rates while increasing motility, especially at higher concentrations. NaCl supplementation and osmolarity had no significant effect on any of the sperm quality parameters. In conclusion, pyruvate maintains a higher quality and motility of sperm stored at 17 °C in PBS in comparison with a glucose-supplemented extender. 2025-07-03T06:33:09Z 2025-07-03T06:33:09Z 2025 article publishedVersion Gacem, S.; Mocé, E.; Gozalbo, C.; Albuixech-Benetó, M.; Esteve, I.C.; Martínez-Talaván, A.; Silvestre, M.A. The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C. Biology 2025, 14, 782. https://hdl.handle.net/20.500.11939/9076 10.3390/ biology14070782 https://www.mdpi.com/2079-7737/14/7/782 en AGROALNEXT/2022/063 IVIA 71714 CIAPOT/2022/14 info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52201/ES/Relanzando la agroalimentación valenciana para una producción y consumo sostenibles y seguros/AgroAlimVal openAccess MPDI electronico |
| spellingShingle | L53 Animal physiology - Reproduction Pyruvates Lactates Fructose Glucose Sperm Goats Gacem, Sabrina Mocé, Eva Gozalbo, Carmen Albuixech-Benetó, Marta ESTEVE, INÉS CAROLINA Martínez-Talaván, Amparo Silvestre, Miguel Ángel The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C |
| title | The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C |
| title_full | The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C |
| title_fullStr | The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C |
| title_full_unstemmed | The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C |
| title_short | The Effects of Extender Energetic Substrate Type on Goat Sperm Stored at 17 ◦C |
| title_sort | effects of extender energetic substrate type on goat sperm stored at 17 ◦c |
| topic | L53 Animal physiology - Reproduction Pyruvates Lactates Fructose Glucose Sperm Goats |
| url | https://hdl.handle.net/20.500.11939/9076 https://www.mdpi.com/2079-7737/14/7/782 |
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