Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus
Background/Objective: Salinity is a growing problem affecting a large portion of global agricultural land, particularly in areas where water resources are scarce. The objective of this study was to provide physiological and molecular information on salttolerant citrus rootstocks to mitigate the d...
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| Format: | article |
| Language: | Inglés |
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MDPI
2025
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| Online Access: | https://hdl.handle.net/20.500.11939/9067 https://www.mdpi.com/2073-4425/16/6/683 |
| _version_ | 1855032905751855104 |
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| author | Asins, María J. Raga, Verónica Romero-Aranda, María R. Jaime-Fernández, Emilio Carbonell, Emilio A. Belver, Andres |
| author_browse | Asins, María J. Belver, Andres Carbonell, Emilio A. Jaime-Fernández, Emilio Raga, Verónica Romero-Aranda, María R. |
| author_facet | Asins, María J. Raga, Verónica Romero-Aranda, María R. Jaime-Fernández, Emilio Carbonell, Emilio A. Belver, Andres |
| author_sort | Asins, María J. |
| collection | ReDivia |
| description | Background/Objective: Salinity is a growing problem affecting a large portion
of global agricultural land, particularly in areas where water resources are scarce. The
objective of this study was to provide physiological and molecular information on salttolerant
citrus rootstocks to mitigate the detrimental effects of salinity on citriculture.
Methods: Ten accessions belonging to eight Citrus species and four to Poncirus trifoliata
Raf. were tested for salinity tolerance (0 and 15 mM NaCl for 1 year) in terms of vegetative
and Cl− tissue distribution traits. In addition, most accessions were evaluated for leaf
Na+ and other cations. Results: All salt tolerant accessions tended to restrict the leaf Cl−
content, although in a lower degree than the Cleopatra mandarin. However, differences in
their ability to restrict leaf [Na+] were evident, contributing to a classification of trifoliate
and sour orange accessions that matched their genotypic grouping based on allele sharing
at a marker targeting candidate gene coding for the NPF5.9 transporter within LCL-6
quantitative trait locus. Conclusions: Our markers targeting LCl-6 candidate genes coding
for NPF5.9, PIP2.1, and CHX20 (citrus GmSALT3 ortholog) could be efficient tools for
managing the detected salt tolerance diversity in terms of both Cl− and Na+ homeostasis
in rootstock breeding programs derived from these species, in addition to Citrus reshni. |
| format | article |
| id | ReDivia9067 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia90672025-06-03T18:38:12Z Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus Asins, María J. Raga, Verónica Romero-Aranda, María R. Jaime-Fernández, Emilio Carbonell, Emilio A. Belver, Andres rootstock breeding germplasm enhancement NPF5.9 CHX20 Cl− homeostasis Na+ homeostasis F30 Plant genetics and breeding Poncirus QTL (quantitative trait loci) Background/Objective: Salinity is a growing problem affecting a large portion of global agricultural land, particularly in areas where water resources are scarce. The objective of this study was to provide physiological and molecular information on salttolerant citrus rootstocks to mitigate the detrimental effects of salinity on citriculture. Methods: Ten accessions belonging to eight Citrus species and four to Poncirus trifoliata Raf. were tested for salinity tolerance (0 and 15 mM NaCl for 1 year) in terms of vegetative and Cl− tissue distribution traits. In addition, most accessions were evaluated for leaf Na+ and other cations. Results: All salt tolerant accessions tended to restrict the leaf Cl− content, although in a lower degree than the Cleopatra mandarin. However, differences in their ability to restrict leaf [Na+] were evident, contributing to a classification of trifoliate and sour orange accessions that matched their genotypic grouping based on allele sharing at a marker targeting candidate gene coding for the NPF5.9 transporter within LCL-6 quantitative trait locus. Conclusions: Our markers targeting LCl-6 candidate genes coding for NPF5.9, PIP2.1, and CHX20 (citrus GmSALT3 ortholog) could be efficient tools for managing the detected salt tolerance diversity in terms of both Cl− and Na+ homeostasis in rootstock breeding programs derived from these species, in addition to Citrus reshni. 2025-06-03T18:38:11Z 2025-06-03T18:38:11Z 2025 article publishedVersion Asins, M. J., Raga, M. V., Romero-Aranda, M. R., Jaime-Fernández, E., Carbonell, E. A., & Belver, A. (2025). Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus. Genes. 2073-4425 https://hdl.handle.net/20.500.11939/9067 10.3390/ genes16060683 https://www.mdpi.com/2073-4425/16/6/683 en info:eu-repo/grantAgreement/AEI/Proyectos de Generación de Conocimiento/PID2021-124599OB-100 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | rootstock breeding germplasm enhancement NPF5.9 CHX20 Cl− homeostasis Na+ homeostasis F30 Plant genetics and breeding Poncirus QTL (quantitative trait loci) Asins, María J. Raga, Verónica Romero-Aranda, María R. Jaime-Fernández, Emilio Carbonell, Emilio A. Belver, Andres Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus |
| title | Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus |
| title_full | Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus |
| title_fullStr | Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus |
| title_full_unstemmed | Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus |
| title_short | Salt Tolerance Diversity in Citrus Rootstocks Agrees with Genotypic Diversity at the LCl-6 Quantitative Trait Locus |
| title_sort | salt tolerance diversity in citrus rootstocks agrees with genotypic diversity at the lcl 6 quantitative trait locus |
| topic | rootstock breeding germplasm enhancement NPF5.9 CHX20 Cl− homeostasis Na+ homeostasis F30 Plant genetics and breeding Poncirus QTL (quantitative trait loci) |
| url | https://hdl.handle.net/20.500.11939/9067 https://www.mdpi.com/2073-4425/16/6/683 |
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