Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus

Improving nitrogen uptake efficiency by citrus in Mediterranean areas, where this crop predominates, is crucial for reducing ground-water pollution and enhancing environmental sustainability. This aligns with the Farm to Fork Strategy (European Green Deal) objectives, which aim to reduce the use...

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Main Authors: Pérez-Piqueres, Ana, Martínez-Alcatára, Belén, Canet, Rodolfo, del Val, Raquel, Quinones, Ana
Format: Artículo
Language:Inglés
Published: PLOS 2025
Subjects:
Online Access:https://hdl.handle.net/20.500.11939/9040
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0311400
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author Pérez-Piqueres, Ana
Martínez-Alcatára, Belén
Canet, Rodolfo
del Val, Raquel
Quinones, Ana
author_browse Canet, Rodolfo
Martínez-Alcatára, Belén
Pérez-Piqueres, Ana
Quinones, Ana
del Val, Raquel
author_facet Pérez-Piqueres, Ana
Martínez-Alcatára, Belén
Canet, Rodolfo
del Val, Raquel
Quinones, Ana
author_sort Pérez-Piqueres, Ana
collection ReDivia
description Improving nitrogen uptake efficiency by citrus in Mediterranean areas, where this crop predominates, is crucial for reducing ground-water pollution and enhancing environmental sustainability. This aligns with the Farm to Fork Strategy (European Green Deal) objectives, which aim to reduce the use of mineral fertilizers by up to 20% and to eliminate soil contamination from nitrogen entirely. In this context, exploring the potential of plant growth-promoting bacteria application to reduce nutrient inputs is a promising opportunity. The objective of the present study was to evaluate the effect of two Bacillus subtilis strains either individually inoculated or in combination with Saccharomyces cerevisiae on 15N-labeled fertilizer uptake efficiency and physiological parameters. Individual inoculations positively affected tree water potential, leaf chlorophyll concentrations (SPAD-values) and photosynthetic performance, enhancing tree growth. Fertilizer-15N use efficiency increased, as did phosphorus and potassium uptakes. Conversely, no response was observed in the trees co-inoculated with S cerevisiae. Therefore, PGPB can be considered an interesting means to reduce reliance on synthetic fertilizers in citrus orchards, minimizing the environmental impact and promoting sustainable production practices.
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institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
language Inglés
publishDate 2025
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spelling ReDivia90402025-04-25T14:49:52Z Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus Pérez-Piqueres, Ana Martínez-Alcatára, Belén Canet, Rodolfo del Val, Raquel Quinones, Ana F61 Plant physiology - Nutrition Fertilizers Citrus Trees Leaves Chlorophylls Saccharomyces cerevisiae Nutrients Bacillus Improving nitrogen uptake efficiency by citrus in Mediterranean areas, where this crop predominates, is crucial for reducing ground-water pollution and enhancing environmental sustainability. This aligns with the Farm to Fork Strategy (European Green Deal) objectives, which aim to reduce the use of mineral fertilizers by up to 20% and to eliminate soil contamination from nitrogen entirely. In this context, exploring the potential of plant growth-promoting bacteria application to reduce nutrient inputs is a promising opportunity. The objective of the present study was to evaluate the effect of two Bacillus subtilis strains either individually inoculated or in combination with Saccharomyces cerevisiae on 15N-labeled fertilizer uptake efficiency and physiological parameters. Individual inoculations positively affected tree water potential, leaf chlorophyll concentrations (SPAD-values) and photosynthetic performance, enhancing tree growth. Fertilizer-15N use efficiency increased, as did phosphorus and potassium uptakes. Conversely, no response was observed in the trees co-inoculated with S cerevisiae. Therefore, PGPB can be considered an interesting means to reduce reliance on synthetic fertilizers in citrus orchards, minimizing the environmental impact and promoting sustainable production practices. 2025-03-11T09:13:14Z 2025-03-11T09:13:14Z 2025 article publishedVersion Pérez-Piqueres, A., Martínez-Alcántara, B., Canet, R., del Val, R., & Quiñones, A. (2025). Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus. Plos One 1932-6203 https://hdl.handle.net/20.500.11939/9040 10.1371/journal.pone.0311400 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0311400 en This study was carried out in the frame of the collaboration agreement between the enterprise: IAB, Investigaciones y Aplicaciones Biotecnolo´gicas, S.L. and Instituto Valenciano de Investigaciones Agrarias: “Biostimulating bacteria as natural growth stimulators and nutritional status improvers” (Project number IVIA 7212) R.V. was an employee of IAB, Investigaciones y Aplicaciones Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess PLOS electronico
spellingShingle F61 Plant physiology - Nutrition
Fertilizers
Citrus
Trees
Leaves
Chlorophylls
Saccharomyces cerevisiae
Nutrients
Bacillus
Pérez-Piqueres, Ana
Martínez-Alcatára, Belén
Canet, Rodolfo
del Val, Raquel
Quinones, Ana
Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus
title Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus
title_full Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus
title_fullStr Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus
title_full_unstemmed Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus
title_short Plant growth-promoting microorganisms as natural stimulators of nitrogen uptake in citrus
title_sort plant growth promoting microorganisms as natural stimulators of nitrogen uptake in citrus
topic F61 Plant physiology - Nutrition
Fertilizers
Citrus
Trees
Leaves
Chlorophylls
Saccharomyces cerevisiae
Nutrients
Bacillus
url https://hdl.handle.net/20.500.11939/9040
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0311400
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