Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants
Grafting onto pepper rootstock NIBER® is an effective strategy to mitigate water stress effects on the grafted variety. In this work, we comparatively explored the metabolomic responses to water stress in the pepper variety “Maestral F1” (V) grafted onto NIBER® (V/N) and self-grafted (V/V) by unta...
| Autores principales: | , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
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ELSEVIER
2025
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| Acceso en línea: | https://hdl.handle.net/20.500.11939/9026 https://www.sciencedirect.com/science/article/pii/S2667064X24001957 |
| _version_ | 1855492609640759296 |
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| author | Padilla, Yaiza Gara Miras Moreno, Begoña Gisbert-Mullor, Ramón Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles |
| author_browse | Calatayud, Ángeles Gisbert-Mullor, Ramón Lucini, Luigi López-Galarza, Salvador Miras Moreno, Begoña Padilla, Yaiza Gara |
| author_facet | Padilla, Yaiza Gara Miras Moreno, Begoña Gisbert-Mullor, Ramón Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles |
| author_sort | Padilla, Yaiza Gara |
| collection | ReDivia |
| description | Grafting onto pepper rootstock NIBER® is an effective strategy to mitigate water stress effects on the grafted
variety. In this work, we comparatively explored the metabolomic responses to water stress in the pepper variety
“Maestral F1” (V) grafted onto NIBER® (V/N) and self-grafted (V/V) by untargeted metabolomics on leaves and
roots. Leaf water status was also evaluated by relative water content (RWC) and gas exchange measurements.
Under water stress, the V/N water use efficiency (WUE) and leaf RWC were higher than V/V, in agreement with
major stomata closure and water retention in leaves. V/N showed a tolerance response, which was manifested in
the untargeted metabolomic analysis. NIBER® modulated the grafted variety response to water stress as reflected
in the differential metabolomic profiles in leaves and roots. The V/N-enriched metabolic pathways showed that
the NIBER® response to water stress involved cutin and suberin biosynthesis, which act as protection layers, and
jasmonic acid (JA) and jasmonates biosynthesis to favor signaling pathways. NIBER® did not induce flavonols
and chlorophyll b synthesis, but likely promoted anthocyanins biosynthesis and maintained an undisturbed
chlorophyll a:chlorophyll b ratio. Moreover, NIBER® increased vitamin B6, anthocyanins and stearic acid concentration
in the variety leaves, whereas siroheme content rose in roots to improve nitrogen assimilation. Further
studies are required to understand the contribution of secondary metabolites, such as phenylpropanoids, glycoalkaloids,
and nitrogen-containing secondary metabolites, to NIBER® water stress tolerance. |
| format | Artículo |
| id | ReDivia9026 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | ELSEVIER |
| publisherStr | ELSEVIER |
| record_format | dspace |
| spelling | ReDivia90262025-04-25T14:49:48Z Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants Padilla, Yaiza Gara Miras Moreno, Begoña Gisbert-Mullor, Ramón Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles Untargeted metabolomics F30 Plant genetics and breeding Drought Grafting Metabolic pathway Pepper Tolerance Grafting onto pepper rootstock NIBER® is an effective strategy to mitigate water stress effects on the grafted variety. In this work, we comparatively explored the metabolomic responses to water stress in the pepper variety “Maestral F1” (V) grafted onto NIBER® (V/N) and self-grafted (V/V) by untargeted metabolomics on leaves and roots. Leaf water status was also evaluated by relative water content (RWC) and gas exchange measurements. Under water stress, the V/N water use efficiency (WUE) and leaf RWC were higher than V/V, in agreement with major stomata closure and water retention in leaves. V/N showed a tolerance response, which was manifested in the untargeted metabolomic analysis. NIBER® modulated the grafted variety response to water stress as reflected in the differential metabolomic profiles in leaves and roots. The V/N-enriched metabolic pathways showed that the NIBER® response to water stress involved cutin and suberin biosynthesis, which act as protection layers, and jasmonic acid (JA) and jasmonates biosynthesis to favor signaling pathways. NIBER® did not induce flavonols and chlorophyll b synthesis, but likely promoted anthocyanins biosynthesis and maintained an undisturbed chlorophyll a:chlorophyll b ratio. Moreover, NIBER® increased vitamin B6, anthocyanins and stearic acid concentration in the variety leaves, whereas siroheme content rose in roots to improve nitrogen assimilation. Further studies are required to understand the contribution of secondary metabolites, such as phenylpropanoids, glycoalkaloids, and nitrogen-containing secondary metabolites, to NIBER® water stress tolerance. 2025-02-17T11:58:48Z 2025-02-17T11:58:48Z 2024 article publishedVersion Padilla, Y. G., Miras-Moreno, B., Gisbert-Mullor, R., Lucini, L., López-Galarza, S., & Calatayud, Á. (2024). Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants. Plant Stress, 13, 100542. 2667-064X https://hdl.handle.net/20.500.11939/9026 10.1016/j.stress.2024.100542 https://www.sciencedirect.com/science/article/pii/S2667064X24001957 en This work has been financed by Grant PID2020-118824RR-C21, funded by MCIN/AEI/10.13039/501100011033. Ram´on Gisbert- Mullor is a beneficiary of a doctoral fellowship (FPU-MEFP (Spain)). Yaiza Gara Padilla is a beneficiary of grant PRE2018-086374 funded by MCIN/AEI/10.13039/501100011033 and, as appropriate, by “ESF Investing in your future”. info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/PID2020-118824RR-C21/ES/IDENTIFICACION Y CARACTERIZACION DE LAS BASES MOLECULARES Y FISIOLOGICAS EN LA UTILIZACION DE PATRONES DE PIMIENTO TOLERANTES A TEMPERATURAS NO OPTIMAS Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess ELSEVIER electronico |
| spellingShingle | Untargeted metabolomics F30 Plant genetics and breeding Drought Grafting Metabolic pathway Pepper Tolerance Padilla, Yaiza Gara Miras Moreno, Begoña Gisbert-Mullor, Ramón Lucini, Luigi López-Galarza, Salvador Calatayud, Ángeles Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants |
| title | Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants |
| title_full | Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants |
| title_fullStr | Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants |
| title_full_unstemmed | Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants |
| title_short | Leaves and roots metabolomic signatures underlying rootstock-mediated water stress tolerance in grafted pepper plants |
| title_sort | leaves and roots metabolomic signatures underlying rootstock mediated water stress tolerance in grafted pepper plants |
| topic | Untargeted metabolomics F30 Plant genetics and breeding Drought Grafting Metabolic pathway Pepper Tolerance |
| url | https://hdl.handle.net/20.500.11939/9026 https://www.sciencedirect.com/science/article/pii/S2667064X24001957 |
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