Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection
Green mold caused by Penicillium digitatum (Pers.:Fr.) Sacc is the most prevalent postharvest rot concerning citrus fruits. Using the subtractive suppression hybridization (SSH) technique, different P. digitatum genes have been identified that could be involved in virulence during citrus infectio...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
| Publicado: |
MDPI
2025
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/9023 https://www.mdpi.com/2309-608X/10/4/235 |
| _version_ | 1855032897447133184 |
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| author | Sánchez-Torres, Paloma Gonzalez-Candelas, Luis Ballester, Ana Rosa |
| author_browse | Ballester, Ana Rosa Gonzalez-Candelas, Luis Sánchez-Torres, Paloma |
| author_facet | Sánchez-Torres, Paloma Gonzalez-Candelas, Luis Ballester, Ana Rosa |
| author_sort | Sánchez-Torres, Paloma |
| collection | ReDivia |
| description | Green mold caused by Penicillium digitatum (Pers.:Fr.) Sacc is the most prevalent postharvest
rot concerning citrus fruits. Using the subtractive suppression hybridization (SSH) technique,
different P. digitatum genes have been identified that could be involved in virulence during citrus
infection in the early stages, a crucial moment that determines whether the infection progresses or not.
To this end, a comparison of two P. digitatum strains with high and low virulence has been carried
out. We conducted a study on the gene expression profile of the most relevant genes. The results
indicate the importance of transcription and regulation processes as well as enzymes involved in the
degradation of the plant cell wall. The most represented expressed sequence tag (EST) was identified
as PDIP_11000, associated with the FluG domain, which is putatively involved in the activation of
conidiation. It is also worth noting that PDIP_02280 encodes a pectin methyl esterase, a cell wall
remodeling protein with a high expression level in the most virulent fungal strains, which is notably
induced during citrus infection. Furthermore, within the group with the greatest representation
and showing significant induction in the early stages of infection, regulatory proteins (PDIP_68700,
PDIP_76160) and a chaperone (PDIP_38040) stand out. To a lesser extent, but not less relevant, it is
worth distinguishing different regulatory proteins and transcription factors, such as PDIP_00580,
PDIP_49640 and PDIP_78930. |
| format | article |
| id | ReDivia9023 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2025 |
| publishDateRange | 2025 |
| publishDateSort | 2025 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia90232025-04-25T14:49:47Z Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection Sánchez-Torres, Paloma Gonzalez-Candelas, Luis Ballester, Ana Rosa Citrus Pathogenicity Penicillium digitatum Postharvest Virulence SSH CWDE Regulatory proteins Green mold caused by Penicillium digitatum (Pers.:Fr.) Sacc is the most prevalent postharvest rot concerning citrus fruits. Using the subtractive suppression hybridization (SSH) technique, different P. digitatum genes have been identified that could be involved in virulence during citrus infection in the early stages, a crucial moment that determines whether the infection progresses or not. To this end, a comparison of two P. digitatum strains with high and low virulence has been carried out. We conducted a study on the gene expression profile of the most relevant genes. The results indicate the importance of transcription and regulation processes as well as enzymes involved in the degradation of the plant cell wall. The most represented expressed sequence tag (EST) was identified as PDIP_11000, associated with the FluG domain, which is putatively involved in the activation of conidiation. It is also worth noting that PDIP_02280 encodes a pectin methyl esterase, a cell wall remodeling protein with a high expression level in the most virulent fungal strains, which is notably induced during citrus infection. Furthermore, within the group with the greatest representation and showing significant induction in the early stages of infection, regulatory proteins (PDIP_68700, PDIP_76160) and a chaperone (PDIP_38040) stand out. To a lesser extent, but not less relevant, it is worth distinguishing different regulatory proteins and transcription factors, such as PDIP_00580, PDIP_49640 and PDIP_78930. 2025-02-12T10:53:54Z 2025-02-12T10:53:54Z 2024 article publishedVersion Sánchez-Torres, P., González-Candelas, L., & Ballester, A. R. (2024). Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection. Journal of Fungi, 10(4), 235. 2309-608X https://hdl.handle.net/20.500.11939/9023 10.3390/jof10040235 https://www.mdpi.com/2309-608X/10/4/235 en The authors would like to thank the Spanish Government for financial support through the projects AGL-2008-04828-C03-02, AGL2008-04828-C03-03, AGL2011-30519-C03-01 and AGL2011- 30519-C03-02 from the Ministry of Science and Innovation (Spain). The Accreditation as Center of Excellence Severo Ochoa CEX2021-001189-S funded by MCIN/AEI/10.13039/501100011033 is also fully acknowledged. Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | Citrus Pathogenicity Penicillium digitatum Postharvest Virulence SSH CWDE Regulatory proteins Sánchez-Torres, Paloma Gonzalez-Candelas, Luis Ballester, Ana Rosa Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection |
| title | Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection |
| title_full | Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection |
| title_fullStr | Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection |
| title_full_unstemmed | Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection |
| title_short | Discovery and Transcriptional Profiling of Penicillium digitatum Genes That Could Promote Fungal Virulence during Citrus Fruit Infection |
| title_sort | discovery and transcriptional profiling of penicillium digitatum genes that could promote fungal virulence during citrus fruit infection |
| topic | Citrus Pathogenicity Penicillium digitatum Postharvest Virulence SSH CWDE Regulatory proteins |
| url | https://hdl.handle.net/20.500.11939/9023 https://www.mdpi.com/2309-608X/10/4/235 |
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