Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit

Gonadotropin-releasing hormone (GnRH) -supplemented extenders have emerged as a welfare-orientated method to induce ovulation in the artificial insemination (AI) of rabbits. The main factor that limits the bioavailability of GnRH analogue on intravaginal administration is the proteolytic activ...

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Main Authors: Viudes-De-Castro, María P., Marco-Jiménez, Francisco, Miralles-Bover, H., Vicente, José S.
Format: article
Language:Español
Published: Wiley 2024
Subjects:
Online Access:https://hdl.handle.net/20.500.11939/9001
https://onlinelibrary.wiley.com/doi/full/10.1111/rda.14584
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author Viudes-De-Castro, María P.
Marco-Jiménez, Francisco
Miralles-Bover, H.
Vicente, José S.
author_browse Marco-Jiménez, Francisco
Miralles-Bover, H.
Vicente, José S.
Viudes-De-Castro, María P.
author_facet Viudes-De-Castro, María P.
Marco-Jiménez, Francisco
Miralles-Bover, H.
Vicente, José S.
author_sort Viudes-De-Castro, María P.
collection ReDivia
description Gonadotropin-releasing hormone (GnRH) -supplemented extenders have emerged as a welfare-orientated method to induce ovulation in the artificial insemination (AI) of rabbits. The main factor that limits the bioavailability of GnRH analogue on intravaginal administration is the proteolytic activity of enzymes present in rabbit seminal plasma. The use of GnRH analogues with higher biological potency would allow us to decrease their concentration in the seminal dose without compromising effectiveness. The current study was designed to assess the efficacy of various GnRH analogues concerning their ability to induce ovulation in rabbit AI. The base solution used for experimental extenders contained an aminopeptidase inhibitor. Four experimental groups were used, females from the Control group were induced to ovulate with an intramuscular administration of 1 μg of buserelin, while in the other three groups females received an intravaginal administration of 3.5 μg of buserelin (BUS), deslorelin (DES) or fertirelin (FER) within the seminal dose. Results showed that the ovulation frequency was similar in all groups studied. A concentration of 3.5 μg of the different GnRH analogues tested in this study showed similar potency in inducing ovulation in non-lactating females, yielding comparable results in terms of pregnancy rate at birth and prolificacy.
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spelling ReDivia90012025-04-25T14:49:36Z Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit Viudes-De-Castro, María P. Marco-Jiménez, Francisco Miralles-Bover, H. Vicente, José S. GnRH analogues L53 Animal physiology - Reproduction Induced ovulation Artificial insemination Gonadotropin-releasing hormone (GnRH) -supplemented extenders have emerged as a welfare-orientated method to induce ovulation in the artificial insemination (AI) of rabbits. The main factor that limits the bioavailability of GnRH analogue on intravaginal administration is the proteolytic activity of enzymes present in rabbit seminal plasma. The use of GnRH analogues with higher biological potency would allow us to decrease their concentration in the seminal dose without compromising effectiveness. The current study was designed to assess the efficacy of various GnRH analogues concerning their ability to induce ovulation in rabbit AI. The base solution used for experimental extenders contained an aminopeptidase inhibitor. Four experimental groups were used, females from the Control group were induced to ovulate with an intramuscular administration of 1 μg of buserelin, while in the other three groups females received an intravaginal administration of 3.5 μg of buserelin (BUS), deslorelin (DES) or fertirelin (FER) within the seminal dose. Results showed that the ovulation frequency was similar in all groups studied. A concentration of 3.5 μg of the different GnRH analogues tested in this study showed similar potency in inducing ovulation in non-lactating females, yielding comparable results in terms of pregnancy rate at birth and prolificacy. 2024-11-04T13:17:45Z 2024-11-04T13:17:45Z 2024 article acceptedVersion Viudes‐de‐Castro, M. P., Marco‐Jiménez, F., Miralles‐Bover, H., & Vicente, J. S. (2024). Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit. Reproduction in Domestic Animals, 59, e14584. https://hdl.handle.net/20.500.11939/9001 10.1111/rda.14584 https://onlinelibrary.wiley.com/doi/full/10.1111/rda.14584 es info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52201/ES/Relanzando la agroalimentación valenciana para una producción y consumo sostenibles y seguros/AgroAlimVal info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2021-127867OB-100 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Wiley electronico
spellingShingle GnRH analogues
L53 Animal physiology - Reproduction
Induced ovulation
Artificial insemination
Viudes-De-Castro, María P.
Marco-Jiménez, Francisco
Miralles-Bover, H.
Vicente, José S.
Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit
title Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit
title_full Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit
title_fullStr Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit
title_full_unstemmed Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit
title_short Potency evaluation of different GNRH analogues on ovulation induction and reproductive performance of doe rabbit
title_sort potency evaluation of different gnrh analogues on ovulation induction and reproductive performance of doe rabbit
topic GnRH analogues
L53 Animal physiology - Reproduction
Induced ovulation
Artificial insemination
url https://hdl.handle.net/20.500.11939/9001
https://onlinelibrary.wiley.com/doi/full/10.1111/rda.14584
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