Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually
Citrus black spot (CBS) caused by Phyllosticta citricarpa was reported for the first time in Tunisia in 2019. This was also the first reported occurrence of the disease in a Mediterranean climate. In Tunisia, CBS is mainly found in lemon (Citrus limon) orchards, and is seldom observed on sweet orang...
| Autores principales: | , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Elsevier
2024
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| Acceso en línea: | https://hdl.handle.net/20.500.11939/8977 https://www.sciencedirect.com/science/article/abs/pii/S1878614624000497?via%3Dihub |
| _version_ | 1855032889237831680 |
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| author | Ioos, Renaud Mannai, Sabrine Jeandel, Céline Benfradj, Najwa Vicent, Antonio Boughalleb-M'hamdi, Naima Aguayo, Jaime |
| author_browse | Aguayo, Jaime Benfradj, Najwa Boughalleb-M'hamdi, Naima Ioos, Renaud Jeandel, Céline Mannai, Sabrine Vicent, Antonio |
| author_facet | Ioos, Renaud Mannai, Sabrine Jeandel, Céline Benfradj, Najwa Vicent, Antonio Boughalleb-M'hamdi, Naima Aguayo, Jaime |
| author_sort | Ioos, Renaud |
| collection | ReDivia |
| description | Citrus black spot (CBS) caused by Phyllosticta citricarpa was reported for the first time in Tunisia in 2019. This was also the first reported occurrence of the disease in a Mediterranean climate. In Tunisia, CBS is mainly found in lemon (Citrus limon) orchards, and is seldom observed on sweet orange (Citrus × sinensis). This recent finding in North Africa raises questions about how the disease has been able to spread under Mediterranean climatic conditions. In this work, 216 Phyllosticta strains collected from lemon orchards in 2021, 2022 and 2023 throughout the country's main citrus-growing provinces were characterised by species morphological and molecular identification, mating type and Simple Sequence Repeats (SSR) microsatellite genotyping (MLG). P. citricarpa was the only species found to be associated with CBS in Tunisia. Although P. citricarpa is a heterothallic fungal species, potentially able to reproduce both sexually and asexually, a single mating type (MAT 1-1-1) idiomorph was found in the population. In addition, three MLGs were observed, across ten microsatellite loci, one of which was massively represented (93 %), indicating a clonal population. The clonality observed suggests a single recent introduction of the pathogen into the country. These findings support the idea that in Tunisia, P. citricarpa only reproduces asexually by pycniospores, with a relatively limited dispersal potential. This is consistent with the absence of pseudothecia on the leaf litter. These results show that CBS is able to thrive under Mediterranean conditions, even in the absence of sexual reproduction. This should be taken into consideration for CBS risk assessment and management. |
| format | article |
| id | ReDivia8977 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia89772025-04-25T14:49:41Z Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually Ioos, Renaud Mannai, Sabrine Jeandel, Céline Benfradj, Najwa Vicent, Antonio Boughalleb-M'hamdi, Naima Aguayo, Jaime Fungus Citrus disease Citrus black spot H20 Plant diseases Population genetics Quarantine Citrus black spot (CBS) caused by Phyllosticta citricarpa was reported for the first time in Tunisia in 2019. This was also the first reported occurrence of the disease in a Mediterranean climate. In Tunisia, CBS is mainly found in lemon (Citrus limon) orchards, and is seldom observed on sweet orange (Citrus × sinensis). This recent finding in North Africa raises questions about how the disease has been able to spread under Mediterranean climatic conditions. In this work, 216 Phyllosticta strains collected from lemon orchards in 2021, 2022 and 2023 throughout the country's main citrus-growing provinces were characterised by species morphological and molecular identification, mating type and Simple Sequence Repeats (SSR) microsatellite genotyping (MLG). P. citricarpa was the only species found to be associated with CBS in Tunisia. Although P. citricarpa is a heterothallic fungal species, potentially able to reproduce both sexually and asexually, a single mating type (MAT 1-1-1) idiomorph was found in the population. In addition, three MLGs were observed, across ten microsatellite loci, one of which was massively represented (93 %), indicating a clonal population. The clonality observed suggests a single recent introduction of the pathogen into the country. These findings support the idea that in Tunisia, P. citricarpa only reproduces asexually by pycniospores, with a relatively limited dispersal potential. This is consistent with the absence of pseudothecia on the leaf litter. These results show that CBS is able to thrive under Mediterranean conditions, even in the absence of sexual reproduction. This should be taken into consideration for CBS risk assessment and management. 2024-09-10T08:31:44Z 2024-09-10T08:31:44Z 2024 article publishedVersion Ioos, R., Mannai, S., Jeandel, C., Benfradj, N., Vicent, A., Boughalleb-M'hamdi, N., & Aguayo, J. (2024). Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually. Fungal Biology, 128(3), 1806-1813. 1878-6146 https://hdl.handle.net/20.500.11939/8977 10.1016/j.funbio.2024.04.004 R https://www.sciencedirect.com/science/article/abs/pii/S1878614624000497?via%3Dihub en This work was supported by a grant from the European Food Safety Authority (EFSA) “GP/EFSA/ALPHA/2019/04 Reduce risk assessment uncertainty: suitability of Mediterranean citrus production areas for Phyllosticta citricarpa”. The mycology research unit of the ANSES Plant Health Laboratory (LSV), USC INRAE 1480, is supported by a grant managed by the French National Research Agency (ANR) as part of the French government’s “Investing for the Future” (PIA) programme (ANR-11-LABX-0002-01, Laboratory of Excellence-ARBRE). closedAccess Elsevier electronico |
| spellingShingle | Fungus Citrus disease Citrus black spot H20 Plant diseases Population genetics Quarantine Ioos, Renaud Mannai, Sabrine Jeandel, Céline Benfradj, Najwa Vicent, Antonio Boughalleb-M'hamdi, Naima Aguayo, Jaime Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually |
| title | Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually |
| title_full | Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually |
| title_fullStr | Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually |
| title_full_unstemmed | Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually |
| title_short | Mating type and microsatellite genotyping indicate that the Tunisian population of Phyllosticta citricarpa is clonal and thrives only asexually |
| title_sort | mating type and microsatellite genotyping indicate that the tunisian population of phyllosticta citricarpa is clonal and thrives only asexually |
| topic | Fungus Citrus disease Citrus black spot H20 Plant diseases Population genetics Quarantine |
| url | https://hdl.handle.net/20.500.11939/8977 https://www.sciencedirect.com/science/article/abs/pii/S1878614624000497?via%3Dihub |
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