Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study
In order to explore a less stressful and hormone-independent insemination method in rabbits, we aimed to evaluate the influence of a new insemination cannula on reproductive performance. Two assays were conducted. In the first, 136 nulliparous and multiparous females were treated with 12.5 IU of eCG...
| Autores principales: | , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Elsevier
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8973 https://www.sciencedirect.com/science/article/pii/S1871141324000829 |
| _version_ | 1855032888456642560 |
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| author | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José S. |
| author_browse | Marco-Jiménez, Francisco Vicente, José S. Viudes-De-Castro, María P. |
| author_facet | Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José S. |
| author_sort | Viudes-De-Castro, María P. |
| collection | ReDivia |
| description | In order to explore a less stressful and hormone-independent insemination method in rabbits, we aimed to evaluate the influence of a new insemination cannula on reproductive performance. Two assays were conducted. In the first, 136 nulliparous and multiparous females were treated with 12.5 IU of eCG to improve their receptivity and divided into four stimulation groups (aGnRHsingle bondControl, Extender, Seminal and Sperm) to test ovulation frequency and rate. The control group received 1 µg of buserelin acetate intramuscularly to induce ovulation. The other groups used a 3D printed cannula to introduce 0.5 ml of extender, undiluted seminal plasma or resuspended sperm into the vagina. After six days, laparoscopy was performed to assess ovulation frequency and rate. Results showed that nulliparous does exhibited higher ovulation frequencies compared to multiparous does, regardless of the stimulation group.
In the second assay, reproductive performance (delivery rate and litter size at birth) of 109 females treated with 12.5 IU of eCG to improve their receptivity was studied after insemination with a seminal dose using the 3D printed cannula. Forty-nine of them were induced to ovulate with buserelin acetate as a control. The results confirmed previous findings, as cannula stimulation allowed better delivery rates in receptive nulliparous than multiparous does. Differential response to multiparous does to the physical stimulus of the cannula could be supported by the histological changes and loss of contractile response that occur after multiple births. Litter sizes at birth were similar between experimental groups.
In conclusion, the 3D designed cannula showed promising results in nulliparous does in ovulating frequency, and further refinement is needed to enhance its effectiveness, particularly in multiparous rabbits. |
| format | article |
| id | ReDivia8973 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia89732025-04-25T14:49:44Z Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José S. Reproductive status Ovulation frequency Insemination 3D printed cannula L53 Animal physiology - Reproduction Rabbits In order to explore a less stressful and hormone-independent insemination method in rabbits, we aimed to evaluate the influence of a new insemination cannula on reproductive performance. Two assays were conducted. In the first, 136 nulliparous and multiparous females were treated with 12.5 IU of eCG to improve their receptivity and divided into four stimulation groups (aGnRHsingle bondControl, Extender, Seminal and Sperm) to test ovulation frequency and rate. The control group received 1 µg of buserelin acetate intramuscularly to induce ovulation. The other groups used a 3D printed cannula to introduce 0.5 ml of extender, undiluted seminal plasma or resuspended sperm into the vagina. After six days, laparoscopy was performed to assess ovulation frequency and rate. Results showed that nulliparous does exhibited higher ovulation frequencies compared to multiparous does, regardless of the stimulation group. In the second assay, reproductive performance (delivery rate and litter size at birth) of 109 females treated with 12.5 IU of eCG to improve their receptivity was studied after insemination with a seminal dose using the 3D printed cannula. Forty-nine of them were induced to ovulate with buserelin acetate as a control. The results confirmed previous findings, as cannula stimulation allowed better delivery rates in receptive nulliparous than multiparous does. Differential response to multiparous does to the physical stimulus of the cannula could be supported by the histological changes and loss of contractile response that occur after multiple births. Litter sizes at birth were similar between experimental groups. In conclusion, the 3D designed cannula showed promising results in nulliparous does in ovulating frequency, and further refinement is needed to enhance its effectiveness, particularly in multiparous rabbits. 2024-09-03T11:09:07Z 2024-09-03T11:09:07Z 2024 article publishedVersion Viudes-de-Castro, M. P., Marco-Jiménez, F., & Vicente, J. S. (2024). Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study. Livestock Science, 283, 105475. 1878-0490 https://hdl.handle.net/20.500.11939/8973 10.1016/j.livsci.2024.105475 https://www.sciencedirect.com/science/article/pii/S1871141324000829 en Grant PID2021–127867OB-I00 funded by MICIU/AEI/ 10.13039/ 501100011033 and by “ERDF A way of making Europe”. Funding for open access charge: CRUE-Universitat Politecnica de Valencia. Atribución-NoComercial 4.0 Internacional http://creativecommons.org/licenses/by-nc/4.0/ openAccess Elsevier electronico |
| spellingShingle | Reproductive status Ovulation frequency Insemination 3D printed cannula L53 Animal physiology - Reproduction Rabbits Viudes-De-Castro, María P. Marco-Jiménez, Francisco Vicente, José S. Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study |
| title | Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study |
| title_full | Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study |
| title_fullStr | Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study |
| title_full_unstemmed | Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study |
| title_short | Differential ovulation responses in nulliparous and multiparous rabbit females: A cannula-mediated study |
| title_sort | differential ovulation responses in nulliparous and multiparous rabbit females a cannula mediated study |
| topic | Reproductive status Ovulation frequency Insemination 3D printed cannula L53 Animal physiology - Reproduction Rabbits |
| url | https://hdl.handle.net/20.500.11939/8973 https://www.sciencedirect.com/science/article/pii/S1871141324000829 |
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