New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins
The ATP-binding cassette (ABC) transporters are a superfamily of membrane proteins. These active transporters are involved in the export of different substances such as xenobiotics. ABC transporters from subfamily C (ABCC) have also been described as functional receptors for different insecticidal p...
| Autores principales: | , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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MDPI
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8968 https://www.mdpi.com/2218-273X/14/4/397 |
| _version_ | 1855032887544381440 |
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| author | Pinos, Daniel Millán-Leiva, Anabel Ferré, Juan Hernández-Martínez, Patricia |
| author_browse | Ferré, Juan Hernández-Martínez, Patricia Millán-Leiva, Anabel Pinos, Daniel |
| author_facet | Pinos, Daniel Millán-Leiva, Anabel Ferré, Juan Hernández-Martínez, Patricia |
| author_sort | Pinos, Daniel |
| collection | ReDivia |
| description | The ATP-binding cassette (ABC) transporters are a superfamily of membrane proteins. These active transporters are involved in the export of different substances such as xenobiotics. ABC transporters from subfamily C (ABCC) have also been described as functional receptors for different insecticidal proteins from Bacillus thuringiensis (Bt) in several lepidopteran species. Numerous studies have characterized the relationship between the ABCC2 transporter and Bt Cry1 proteins. Although other ABCC transporters sharing structural and functional similarities have been described, little is known of their role in the mode of action of Bt proteins. For Heliothis virescens, only the ABCC2 transporter and its interaction with Cry1A proteins have been studied to date. Here, we have searched for paralogs to the ABCC2 gene in H. virescens, and identified two new ABC transporter genes: HvABCC3 and HvABCC4. Furthermore, we have characterized their gene expression in the midgut and their protein topology, and compared them with that of ABCC2. Finally, we discuss their possible interaction with Bt proteins by performing protein docking analysis. |
| format | article |
| id | ReDivia8968 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia89682025-04-25T14:49:41Z New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins Pinos, Daniel Millán-Leiva, Anabel Ferré, Juan Hernández-Martínez, Patricia Insecticidal proteins Receptors Tobacco budworm Docking analysis F60 Plant physiology and biochemistry Bacillus thuringiensis The ATP-binding cassette (ABC) transporters are a superfamily of membrane proteins. These active transporters are involved in the export of different substances such as xenobiotics. ABC transporters from subfamily C (ABCC) have also been described as functional receptors for different insecticidal proteins from Bacillus thuringiensis (Bt) in several lepidopteran species. Numerous studies have characterized the relationship between the ABCC2 transporter and Bt Cry1 proteins. Although other ABCC transporters sharing structural and functional similarities have been described, little is known of their role in the mode of action of Bt proteins. For Heliothis virescens, only the ABCC2 transporter and its interaction with Cry1A proteins have been studied to date. Here, we have searched for paralogs to the ABCC2 gene in H. virescens, and identified two new ABC transporter genes: HvABCC3 and HvABCC4. Furthermore, we have characterized their gene expression in the midgut and their protein topology, and compared them with that of ABCC2. Finally, we discuss their possible interaction with Bt proteins by performing protein docking analysis. 2024-08-30T12:00:54Z 2024-08-30T12:00:54Z 2024 article publishedVersion Pinos, D., Millán-Leiva, A., Ferré, J., & Hernández-Martínez, P. (2024). New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins. Biomolecules, 14(4), 397. 2218-273X https://hdl.handle.net/20.500.11939/8968 10.3390/biom14040397 https://www.mdpi.com/2218-273X/14/4/397 en This research was funded by the Spanish Ministry of Science, Innovation and Universities (grant no. RTI2018-095204-B-C21) and the Generalitat Valenciana (grant no. PROMETEO/2020/010). Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | Insecticidal proteins Receptors Tobacco budworm Docking analysis F60 Plant physiology and biochemistry Bacillus thuringiensis Pinos, Daniel Millán-Leiva, Anabel Ferré, Juan Hernández-Martínez, Patricia New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins |
| title | New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins |
| title_full | New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins |
| title_fullStr | New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins |
| title_full_unstemmed | New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins |
| title_short | New Paralogs of the Heliothis virescens ABCC2 Transporter as Potential Receptors for Bt Cry1A Proteins |
| title_sort | new paralogs of the heliothis virescens abcc2 transporter as potential receptors for bt cry1a proteins |
| topic | Insecticidal proteins Receptors Tobacco budworm Docking analysis F60 Plant physiology and biochemistry Bacillus thuringiensis |
| url | https://hdl.handle.net/20.500.11939/8968 https://www.mdpi.com/2218-273X/14/4/397 |
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