High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees
The ecotoxicological consequences of synthetic pesticides have encouraged stakeholders to search for eco-friendly pest control tools, like essential oils (EOs). Nano-delivery systems (nanoparticles and nano-emulsions) seem ideal for developing EO-based biopesticides, although production processes sh...
| Autores principales: | , , , , , , , , |
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| Formato: | Artículo |
| Lenguaje: | Inglés |
| Publicado: |
Springer
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8967 https://link.springer.com/article/10.1007/s10340-024-01800-2#citeas |
| _version_ | 1855492596818771968 |
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| author | Modafferi, Antonino Giunti Urbaneja, Alberto Laudani, Francesca Latella Pérez-Hedo, Mertixell Ricupero, Michele Palmeri, Vince Campolo, Orlando |
| author_browse | Campolo, Orlando Giunti Latella Laudani, Francesca Modafferi, Antonino Palmeri, Vince Pérez-Hedo, Mertixell Ricupero, Michele Urbaneja, Alberto |
| author_facet | Modafferi, Antonino Giunti Urbaneja, Alberto Laudani, Francesca Latella Pérez-Hedo, Mertixell Ricupero, Michele Palmeri, Vince Campolo, Orlando |
| author_sort | Modafferi, Antonino |
| collection | ReDivia |
| description | The ecotoxicological consequences of synthetic pesticides have encouraged stakeholders to search for eco-friendly pest control tools, like essential oils (EOs). Nano-delivery systems (nanoparticles and nano-emulsions) seem ideal for developing EO-based biopesticides, although production processes should be standardized and implemented. In this study, nano-emulsions loaded with a high amount of Allium sativum L. EO (15%) were developed using different mixed bottom-up/top-down processes. Garlic EO was chemically analyzed by gas chromatography-mass spectrometry (GC-MS) and formulations were physically characterized using Dynamic Light Scattering (DLS) apparatus. The insecticidal activity against Planococcus citri Risso (Hemiptera: Pseudococcidae) and selectivity toward Apis mellifera L. (Hymenoptera: Apidae) worker bees was evaluated. Garlic EO was mainly composed of sulphur components (96.3%), with diallyl disulphide and diallyl trisulphide as the most abundant compounds (37.26% and 28.15%, respectively). Top-down processes could produce stable nano-emulsions with droplet size in the nanometric range (< 200nm) and good polydispersity index (PDI < 0.2). In contrast, the bottom-up emulsion was unstable, and its droplet size was around 500nm after 24 hours. High-energy emulsification processes significantly increased the residual toxicity of garlic EO against 3rd instar P. citri nymphs, whereas the developed formulations were harmless to A. mellifera workers in topical application. This study confirmed that the production process significantly affected the physical properties and efficacy against target pests. The lack of adverse impact on honeybees denotated the potential of these formulations as bioinsecticides in organic and/or IPM programs, although further extended ecotoxicological studies are necessary. |
| format | Artículo |
| id | ReDivia8967 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Springer |
| publisherStr | Springer |
| record_format | dspace |
| spelling | ReDivia89672025-04-25T14:49:40Z High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees Modafferi, Antonino Giunti Urbaneja, Alberto Laudani, Francesca Latella Pérez-Hedo, Mertixell Ricupero, Michele Palmeri, Vince Campolo, Orlando Bioinsecticides Citrus mealybug Ecotoxicology Garlic essential oil Nanotechnology H10 Pests of plants Nanotechnology The ecotoxicological consequences of synthetic pesticides have encouraged stakeholders to search for eco-friendly pest control tools, like essential oils (EOs). Nano-delivery systems (nanoparticles and nano-emulsions) seem ideal for developing EO-based biopesticides, although production processes should be standardized and implemented. In this study, nano-emulsions loaded with a high amount of Allium sativum L. EO (15%) were developed using different mixed bottom-up/top-down processes. Garlic EO was chemically analyzed by gas chromatography-mass spectrometry (GC-MS) and formulations were physically characterized using Dynamic Light Scattering (DLS) apparatus. The insecticidal activity against Planococcus citri Risso (Hemiptera: Pseudococcidae) and selectivity toward Apis mellifera L. (Hymenoptera: Apidae) worker bees was evaluated. Garlic EO was mainly composed of sulphur components (96.3%), with diallyl disulphide and diallyl trisulphide as the most abundant compounds (37.26% and 28.15%, respectively). Top-down processes could produce stable nano-emulsions with droplet size in the nanometric range (< 200nm) and good polydispersity index (PDI < 0.2). In contrast, the bottom-up emulsion was unstable, and its droplet size was around 500nm after 24 hours. High-energy emulsification processes significantly increased the residual toxicity of garlic EO against 3rd instar P. citri nymphs, whereas the developed formulations were harmless to A. mellifera workers in topical application. This study confirmed that the production process significantly affected the physical properties and efficacy against target pests. The lack of adverse impact on honeybees denotated the potential of these formulations as bioinsecticides in organic and/or IPM programs, although further extended ecotoxicological studies are necessary. 2024-08-30T11:48:42Z 2024-08-30T11:48:42Z 2024 article publishedVersion Modafferi, A., Giunti, G., Urbaneja, A., Laudani, F., Latella, I., Pérez-Hedo, M., ... & Campolo, O. (2024). High-energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees. Journal of Pest Science, 1-12. 1612-4766 https://hdl.handle.net/20.500.11939/8967 10.1007/s10340-024-01800-2 https://link.springer.com/article/10.1007/s10340-024-01800-2#citeas en Open access funding provided by Università degli Studi di Salerno within the CRUI-CARE Agreement. This study was carried out within the project ASTER from Call Prima Sect. 2 2021—Multi-Topic, the Agritech National Research Center and received funding from the European Union Next-Generation EU (PIANO NAZIONALE DI RIPRESA E RESILIENZA (PNRR) – MISSIONE 4 COMPONENTE 2, INVESTIMENTO 1.4 – D.D. 1032 17/06/2022, CN00000022). This manuscript reflects only the authors’ views and opinions, neither the European Union nor the European Commission can be considered responsible for them. Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Springer electronico |
| spellingShingle | Bioinsecticides Citrus mealybug Ecotoxicology Garlic essential oil Nanotechnology H10 Pests of plants Nanotechnology Modafferi, Antonino Giunti Urbaneja, Alberto Laudani, Francesca Latella Pérez-Hedo, Mertixell Ricupero, Michele Palmeri, Vince Campolo, Orlando High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees |
| title | High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees |
| title_full | High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees |
| title_fullStr | High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees |
| title_full_unstemmed | High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees |
| title_short | High‑energy emulsification of Allium sativum essential oil boosts insecticidal activity against Planococcus citri with no risk to honeybees |
| title_sort | high energy emulsification of allium sativum essential oil boosts insecticidal activity against planococcus citri with no risk to honeybees |
| topic | Bioinsecticides Citrus mealybug Ecotoxicology Garlic essential oil Nanotechnology H10 Pests of plants Nanotechnology |
| url | https://hdl.handle.net/20.500.11939/8967 https://link.springer.com/article/10.1007/s10340-024-01800-2#citeas |
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