Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning

Fungal infections are a main concern in fruit packing houses since a single infected fruit can spread the infection, causing severe losses. Hence, early detection is crucial. Fluorescence induced by UV light is commonly used to detect infected fruits, but it can harm the eyes and the skin. Hypers...

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Autores principales: Castillo-Gironés, Salvador, Gómez-Sanchis, Juan, López-Chulia, Marina, Guirao, Alberto, Palou, Lluís, Souza, Ricardo-Lima-de, Blasco, José
Formato: poster
Lenguaje:Inglés
Publicado: 2024
Materias:
Acceso en línea:https://hdl.handle.net/20.500.11939/8934
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author Castillo-Gironés, Salvador
Gómez-Sanchis, Juan
López-Chulia, Marina
Guirao, Alberto
Palou, Lluís
Souza, Ricardo-Lima-de
Blasco, José
author_browse Blasco, José
Castillo-Gironés, Salvador
Guirao, Alberto
Gómez-Sanchis, Juan
López-Chulia, Marina
Palou, Lluís
Souza, Ricardo-Lima-de
author_facet Castillo-Gironés, Salvador
Gómez-Sanchis, Juan
López-Chulia, Marina
Guirao, Alberto
Palou, Lluís
Souza, Ricardo-Lima-de
Blasco, José
author_sort Castillo-Gironés, Salvador
collection ReDivia
description Fungal infections are a main concern in fruit packing houses since a single infected fruit can spread the infection, causing severe losses. Hence, early detection is crucial. Fluorescence induced by UV light is commonly used to detect infected fruits, but it can harm the eyes and the skin. Hyperspectral imaging and chemometrics have already been used to detect early infected fruits, but novel deep-learning approaches can improve the detection. In this work, 50 oranges Navelate were inoculated with Penicillium digitatum and another 50 oranges were inoculated with distilled water as a control. After the inoculation, the fruits were stored at 20 ºC and 90 % relative humidity. Images were taken daily from the inoculation for four days, using a Vis-NIR pushbroom system (900-1700 nm). A total of 500 images were captured, corrected using white and dark references, cropped to the size of the fruit and divided into a train (60% of the images), validation (20% of images) and independent test (20% of images) datasets. A customised convolutional nerual network (CNN) was designed, composed of 3 convolutional layers (16, 32 and 64 filters and a kernel size of 3), followed by 3 max-pooling layers (pool size 2) and two final dense layers for classification of 128 and 1 neuron (with sigmoid activation function) which was trained for 800 epochs with a binary cross-entropy loss function. It was possible to correctly classify 100% of the control oranges and 80% of the inoculated ones from the second day, demonstrating that it is possible to detect asymptomatic infected using this technology.
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institution Instituto Valenciano de Investigaciones Agrarias (IVIA)
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spelling ReDivia89342025-04-25T14:54:29Z Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning Castillo-Gironés, Salvador Gómez-Sanchis, Juan López-Chulia, Marina Guirao, Alberto Palou, Lluís Souza, Ricardo-Lima-de Blasco, José Hyperspectral imaging J Handling, transport, storage and protection of agricultural products Citrus Penicillium digitatum machine learning Fungal infections are a main concern in fruit packing houses since a single infected fruit can spread the infection, causing severe losses. Hence, early detection is crucial. Fluorescence induced by UV light is commonly used to detect infected fruits, but it can harm the eyes and the skin. Hyperspectral imaging and chemometrics have already been used to detect early infected fruits, but novel deep-learning approaches can improve the detection. In this work, 50 oranges Navelate were inoculated with Penicillium digitatum and another 50 oranges were inoculated with distilled water as a control. After the inoculation, the fruits were stored at 20 ºC and 90 % relative humidity. Images were taken daily from the inoculation for four days, using a Vis-NIR pushbroom system (900-1700 nm). A total of 500 images were captured, corrected using white and dark references, cropped to the size of the fruit and divided into a train (60% of the images), validation (20% of images) and independent test (20% of images) datasets. A customised convolutional nerual network (CNN) was designed, composed of 3 convolutional layers (16, 32 and 64 filters and a kernel size of 3), followed by 3 max-pooling layers (pool size 2) and two final dense layers for classification of 128 and 1 neuron (with sigmoid activation function) which was trained for 800 epochs with a binary cross-entropy loss function. It was possible to correctly classify 100% of the control oranges and 80% of the inoculated ones from the second day, demonstrating that it is possible to detect asymptomatic infected using this technology. 2024-06-06T11:54:04Z 2024-06-06T11:54:04Z 2023 poster Castillo-Gironés, S., Gómez-Sanchis, J., Lopez-Chulia, M., Guirao-Carrascosa, A., Palou, L., Lima de Souza, R. F., Blasco, J. (2023). Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning. II International Conference Cost Action 19145 SENSORFINT — European Network for assuring food integrity using non-destructive spectral sensors, Berlín, p. 105. [Poster presentation] https://hdl.handle.net/20.500.11939/8934 en 2023-06-05 SensorFINT conference & AK Chemometrik und Qualitätssicherung Annual Workshop Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess electronico
spellingShingle Hyperspectral imaging
J Handling, transport, storage and protection of agricultural products
Citrus
Penicillium digitatum
machine learning
Castillo-Gironés, Salvador
Gómez-Sanchis, Juan
López-Chulia, Marina
Guirao, Alberto
Palou, Lluís
Souza, Ricardo-Lima-de
Blasco, José
Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning
title Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning
title_full Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning
title_fullStr Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning
title_full_unstemmed Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning
title_short Early detection of infection by Penicillium digitatum in oranges using hyperspectral imaging and machine learning
title_sort early detection of infection by penicillium digitatum in oranges using hyperspectral imaging and machine learning
topic Hyperspectral imaging
J Handling, transport, storage and protection of agricultural products
Citrus
Penicillium digitatum
machine learning
url https://hdl.handle.net/20.500.11939/8934
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