Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions

High salinity decreases the productivity of crops worldwide. Pepper is particularly sensitive to high salt concentrations. Herein, we subjected three tolerant pepper accessions (C12, B14 and A25) to high sodium chloride concentration (70 mM NaCl). The aerial and root biomass, leaf and root osmoti...

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Autores principales: López-Serrano, Lidia, Martínez-Cuenca, Mary-Rus, López-Galarza, Salvador, Calatayud, Ángeles
Formato: Artículo
Lenguaje:Inglés
Publicado: Wiley 2024
Materias:
Acceso en línea:https://hdl.handle.net/20.500.11939/8882
https://onlinelibrary.wiley.com/doi/pdf/10.1111/ppl.14090
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author López-Serrano, Lidia
Martínez-Cuenca, Mary-Rus
López-Galarza, Salvador
Calatayud, Ángeles
author_browse Calatayud, Ángeles
López-Galarza, Salvador
López-Serrano, Lidia
Martínez-Cuenca, Mary-Rus
author_facet López-Serrano, Lidia
Martínez-Cuenca, Mary-Rus
López-Galarza, Salvador
Calatayud, Ángeles
author_sort López-Serrano, Lidia
collection ReDivia
description High salinity decreases the productivity of crops worldwide. Pepper is particularly sensitive to high salt concentrations. Herein, we subjected three tolerant pepper accessions (C12, B14 and A25) to high sodium chloride concentration (70 mM NaCl). The aerial and root biomass, leaf and root osmotic potential (Ψπ), Na+, Cl , K+ and proline concentrations and the relative expression of the putative genes CaSOS1, CaHKT1, three CaNHXs and CaP5CS were measured. Different salinity tolerance strategies depending on the pepper accession were identified. In C12, tolerance was attributed to the accumulation of Na+ in vacuoles and endosomes by the activation of vacuolar CaNHXs genes and the reduction in Ψπ; additionally, the activation of CaHKT1 and CaSOS1 in leaves and roots moved and accumulated Na+ ions in the xylem and xylem parenchyma cells (XPC) as well as expulsed it out of the root cells. A25 accession, on the contrary, was specialized in compartmentalizing Na+ ions in root and leaf vacuoles and root XPC by the up-regulation of CaNHXs and CaHKT1, respectively, avoiding a toxic accumulation in leaves. Finally, B14 accession moved and accumulated Na+ in xylem and XPC, reducing its concentration in roots by the activation of CaSOS1 and CaHKT1. This study shade light on different tolerance mechanisms of pepper plants to overcome salt stress
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spelling ReDivia88822025-04-25T14:49:35Z Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions López-Serrano, Lidia Martínez-Cuenca, Mary-Rus López-Galarza, Salvador Calatayud, Ángeles F30 Plant genetics and breeding Pepper Salt stress High salinity decreases the productivity of crops worldwide. Pepper is particularly sensitive to high salt concentrations. Herein, we subjected three tolerant pepper accessions (C12, B14 and A25) to high sodium chloride concentration (70 mM NaCl). The aerial and root biomass, leaf and root osmotic potential (Ψπ), Na+, Cl , K+ and proline concentrations and the relative expression of the putative genes CaSOS1, CaHKT1, three CaNHXs and CaP5CS were measured. Different salinity tolerance strategies depending on the pepper accession were identified. In C12, tolerance was attributed to the accumulation of Na+ in vacuoles and endosomes by the activation of vacuolar CaNHXs genes and the reduction in Ψπ; additionally, the activation of CaHKT1 and CaSOS1 in leaves and roots moved and accumulated Na+ ions in the xylem and xylem parenchyma cells (XPC) as well as expulsed it out of the root cells. A25 accession, on the contrary, was specialized in compartmentalizing Na+ ions in root and leaf vacuoles and root XPC by the up-regulation of CaNHXs and CaHKT1, respectively, avoiding a toxic accumulation in leaves. Finally, B14 accession moved and accumulated Na+ in xylem and XPC, reducing its concentration in roots by the activation of CaSOS1 and CaHKT1. This study shade light on different tolerance mechanisms of pepper plants to overcome salt stress 2024-05-08T11:33:50Z 2024-05-08T11:33:50Z 2023 article acceptedVersion López‐Serrano, L., Martínez‐Cuenca, M. R., López‐Galarza, S., & Calatayud, Á. (2023). Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions. Physiologia Plantarum, 175(6), e14090. 1399-3054 https://hdl.handle.net/20.500.11939/8882 10.1111/ppl.14090 https://onlinelibrary.wiley.com/doi/pdf/10.1111/ppl.14090 en This work was financed by the INIA (Spain) and the Ministerio de Ciencia, Innovacion y Universidades (RTA2017 –00030-C02–00) and the European Regional Development Fund (ERDF). Lidia Lopez- Serrano was a beneficiary of a doctoral fellowship (FPI-INIA) info:eu-repo/grantAgreement/MINECO/Programa estatal de i+D+i Orientada a los Retos de la Sociedad/RTA2017-00030-C02/ES/GENERACIÓN DE PATRONES DE PIMIENTO DIRIGIDOS A LA RESILIENCIA Y ADAPTACIÓN AL ESTRÉS ABIOTICO Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Wiley electronico
spellingShingle F30 Plant genetics and breeding
Pepper
Salt stress
López-Serrano, Lidia
Martínez-Cuenca, Mary-Rus
López-Galarza, Salvador
Calatayud, Ángeles
Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions
title Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions
title_full Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions
title_fullStr Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions
title_full_unstemmed Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions
title_short Differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions
title_sort differential gene expression patterns and physiological responses improve adaptation to high salinity concentration in pepper accessions
topic F30 Plant genetics and breeding
Pepper
Salt stress
url https://hdl.handle.net/20.500.11939/8882
https://onlinelibrary.wiley.com/doi/pdf/10.1111/ppl.14090
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