Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage
Although Nesidiocoris tenuis is highly effective as a biological control agent, it can also damage tomato plants due to its zoophytophagous behavior. When N. tenuis pierces the stems and petioles of tomato plants with its stylets, it triggers callose deposition and subsequent cell death, resulting...
| Main Authors: | , , , , , , |
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| Format: | article |
| Language: | Inglés |
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Elsevier
2024
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| Online Access: | https://hdl.handle.net/20.500.11939/8881 https://www.sciencedirect.com/science/article/pii/S1049964424000823 |
| _version_ | 1855032871565131776 |
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| author | Depalo, Laura Gallego, Carolina Ortells-Fabra, Raúl Salas, Carlos Montalt, Rafael Urbaneja, Alberto Pérez-Hedo, Mertixell |
| author_browse | Depalo, Laura Gallego, Carolina Montalt, Rafael Ortells-Fabra, Raúl Pérez-Hedo, Mertixell Salas, Carlos Urbaneja, Alberto |
| author_facet | Depalo, Laura Gallego, Carolina Ortells-Fabra, Raúl Salas, Carlos Montalt, Rafael Urbaneja, Alberto Pérez-Hedo, Mertixell |
| author_sort | Depalo, Laura |
| collection | ReDivia |
| description | Although Nesidiocoris tenuis is highly effective as a biological control agent, it can also damage tomato plants due
to its zoophytophagous behavior. When N. tenuis pierces the stems and petioles of tomato plants with its stylets, it
triggers callose deposition and subsequent cell death, resulting in blocked nutrient transport, floral abortions, or
wilting of tender shoots. Recently, it has been shown that exposure of tomato plants to the green leaf volatile (Z)-
3-hexenyl propanoate [(Z)-3-HP] activates defensive mechanisms, including the regulation of genes involved in
the synthesis and degradation of callose. In this study, conducted under greenhouse conditions, we tested the
hypothesis that damage caused by N. tenuis could be reduced by exposing tomato plants to (Z)-3-HP through
polymeric dispensers. Tomato plants exposed to (Z)-3-HP and non-exposed control plants were inoculated with
N. tenuis. Nesidiocoris tenuis established in both groups with no significant differences between the two treatments.
However, as hypothesized, the damage caused by N. tenuis was significantly lower in the plants exposed to
(Z)-3-HP. Gene expression analysis of salicylic, jasmonic, and abscisic acids, along with histochemical staining
methods, was used to compare the defensive responses of tomato plants infested solely with N. tenuis versus those
infested with N. tenuis and exposed to (Z)-3-HP. Our findings confirm the influence of (Z)-3-HP exposure on
differential defensive activation between treatments and reduced callose deposition in (Z)-3-HP-exposed plants.
These results pave the way for improved management of N. tenuis by enhancing the plant’s defenses based on inter-plant communication. |
| format | article |
| id | ReDivia8881 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Elsevier |
| publisherStr | Elsevier |
| record_format | dspace |
| spelling | ReDivia88812025-04-25T14:49:35Z Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage Depalo, Laura Gallego, Carolina Ortells-Fabra, Raúl Salas, Carlos Montalt, Rafael Urbaneja, Alberto Pérez-Hedo, Mertixell Necrotic rings Callose deposition HIPV Tissue regeneration Defensive response H10 Pests of plants Biological control Although Nesidiocoris tenuis is highly effective as a biological control agent, it can also damage tomato plants due to its zoophytophagous behavior. When N. tenuis pierces the stems and petioles of tomato plants with its stylets, it triggers callose deposition and subsequent cell death, resulting in blocked nutrient transport, floral abortions, or wilting of tender shoots. Recently, it has been shown that exposure of tomato plants to the green leaf volatile (Z)- 3-hexenyl propanoate [(Z)-3-HP] activates defensive mechanisms, including the regulation of genes involved in the synthesis and degradation of callose. In this study, conducted under greenhouse conditions, we tested the hypothesis that damage caused by N. tenuis could be reduced by exposing tomato plants to (Z)-3-HP through polymeric dispensers. Tomato plants exposed to (Z)-3-HP and non-exposed control plants were inoculated with N. tenuis. Nesidiocoris tenuis established in both groups with no significant differences between the two treatments. However, as hypothesized, the damage caused by N. tenuis was significantly lower in the plants exposed to (Z)-3-HP. Gene expression analysis of salicylic, jasmonic, and abscisic acids, along with histochemical staining methods, was used to compare the defensive responses of tomato plants infested solely with N. tenuis versus those infested with N. tenuis and exposed to (Z)-3-HP. Our findings confirm the influence of (Z)-3-HP exposure on differential defensive activation between treatments and reduced callose deposition in (Z)-3-HP-exposed plants. These results pave the way for improved management of N. tenuis by enhancing the plant’s defenses based on inter-plant communication. 2024-05-08T09:08:04Z 2024-05-08T09:08:04Z 2024 article publishedVersion Depalo, L., Gallego, C., Ortells-Fabra, R., Salas, C., Montalt, R., Urbaneja, A., & Pérez-Hedo, M. (2024). Advancing tomato crop protection: green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage. Biological Control, 105517. 1049-9644 https://hdl.handle.net/20.500.11939/8881 10.1016/j.biocontrol.2024.105517 https://www.sciencedirect.com/science/article/pii/S1049964424000823 en The research leading to these results was partially funded by the Spanish Ministry of Economy and Competitiveness MCIN (PID2020- 113234RR-I00) and by Instituto Valenciano de Investigaciones Agrarias from Conselleria d’Agricultura, Pesca i Alimentaci´o de la Generalitat Valenciana (project “Sostenible” IVIA-52202B, which is susceptible of being co-financed by the European Union through the ERDF Operational Program). R.O.-F. was supported by a Ph.D. contract from MCIN (PRE2021-100879). L.D. was supported by a fellowship awarded by OECD Co-operative Research Programme. info:eu-repo/grantAgreement/MINECO/ Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i y de I+D+i Orientada a los Retos de la Sociedad/PID2020-113234RR-I00/ES/EXPOSICION A VOLATILES INDUCIDOS POR PLANTAS: UNA VIA SOSTENIBLE DE MANEJO DE PLAGAS Y ENFERMEDADES A TRAVES DE LA INDUCCION DEFENSIVA DE LAS PLANTAS info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52202/ES/Control biológico de plagas y enfermedades como pieza clave en el impulso de la Sostenibilidad de los cultivos agrarios de la Comunidad Valenciana/SOSTENIBLE Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Elsevier electronico |
| spellingShingle | Necrotic rings Callose deposition HIPV Tissue regeneration Defensive response H10 Pests of plants Biological control Depalo, Laura Gallego, Carolina Ortells-Fabra, Raúl Salas, Carlos Montalt, Rafael Urbaneja, Alberto Pérez-Hedo, Mertixell Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage |
| title | Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage |
| title_full | Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage |
| title_fullStr | Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage |
| title_full_unstemmed | Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage |
| title_short | Advancing tomato crop protection: Green leaf volatile-mediated defense mechanisms against Nesidiocoris tenuis plant damage |
| title_sort | advancing tomato crop protection green leaf volatile mediated defense mechanisms against nesidiocoris tenuis plant damage |
| topic | Necrotic rings Callose deposition HIPV Tissue regeneration Defensive response H10 Pests of plants Biological control |
| url | https://hdl.handle.net/20.500.11939/8881 https://www.sciencedirect.com/science/article/pii/S1049964424000823 |
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