Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure
Citrus polyploidy is associated with a wide range of morphological, genetic, and physiological changes that are often advantageous for breeding. Citrus triploid hybrids are very interesting as new seedless varieties. However, tetraploid rootstocks promote adaptation to different abiotic stresses and...
| Autores principales: | , , , , , , , |
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| Formato: | article |
| Lenguaje: | Inglés |
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Frontiers Media
2024
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| Materias: | |
| Acceso en línea: | https://hdl.handle.net/20.500.11939/8773 https://www.frontiersin.org/articles/10.3389/fpls.2023.1327872/full |
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| author | Aleza, Pablo Garavello, Miguel Rouiss, Houssem Benedict, Ana Cristina García-Lor, Andrés Hernández, María Navarro, Luis Ollitrault, Patrick |
| author_browse | Aleza, Pablo Benedict, Ana Cristina Garavello, Miguel García-Lor, Andrés Hernández, María Navarro, Luis Ollitrault, Patrick Rouiss, Houssem |
| author_facet | Aleza, Pablo Garavello, Miguel Rouiss, Houssem Benedict, Ana Cristina García-Lor, Andrés Hernández, María Navarro, Luis Ollitrault, Patrick |
| author_sort | Aleza, Pablo |
| collection | ReDivia |
| description | Citrus polyploidy is associated with a wide range of morphological, genetic, and physiological changes that are often advantageous for breeding. Citrus triploid hybrids are very interesting as new seedless varieties. However, tetraploid rootstocks promote adaptation to different abiotic stresses and promote resilience. Triploid and tetraploid hybrids can be obtained through sexual hybridizations using tetraploid parents (2x × 4x, 4x × 2x, or 4x × 4x), but more knowledge is needed about the inheritance pattern of tetraploid parents to optimize the efficiency of triploid varieties and tetraploid rootstock breeding strategies. In this work, we have analyzed the inheritance pattern of three tetraploid genotypes: ‘Chandler’ pummelo (Citrus maxima) and ‘Cleopatra’ mandarin (Citrus reticulata), which represent two clear examples of autotetraploid plants constituted by the genome of a single species, and the ‘Sevillano’ sour orange, which is an allotetraploid interspecific hybrid between C. maxima and C. reticulata. Polymorphic simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers were used to estimate parental heterozygosity restitution, and allele frequencies for centromeric loci were used to calculate the preferential pairing rate related to the proportion of disomic and tetrasomic segregation. The tetraploid pummelo and mandarin displayed tetrasomic segregation. Sour orange evidenced a clear intermediate inheritance for five of the nine chromosomes (1, 2, 5, 7, and 8), a slight tendency toward tetrasomic inheritance on chromosome 3, and intermediate inheritance with a tendency toward disomy for chromosomes 4, 6, and 9. These results indicate that the interspecific versus intraspecific phylogenomic origin affects preferential pairing and, therefore, the inheritance patterns. Despite its high level of heterozygosity, the important preferential chromosome pairing observed in sour orange results in a limited diversity of the genotypic variability of its diploid gametes, and consequently, a large part of the genetic value of the original diploid sour orange is transferred to the tetraploid progenies. |
| format | article |
| id | ReDivia8773 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Frontiers Media |
| publisherStr | Frontiers Media |
| record_format | dspace |
| spelling | ReDivia87732025-04-25T14:49:27Z Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure Aleza, Pablo Garavello, Miguel Rouiss, Houssem Benedict, Ana Cristina García-Lor, Andrés Hernández, María Navarro, Luis Ollitrault, Patrick Triploid Tetrasomic Disomic SSR and SNP markers F30 Plant genetics and breeding F60 Plant physiology and biochemistry F63 Plant physiology - Reproduction Q01 Food science and technology Citrus Seedless varieties Rootstocks Breeding Citrus polyploidy is associated with a wide range of morphological, genetic, and physiological changes that are often advantageous for breeding. Citrus triploid hybrids are very interesting as new seedless varieties. However, tetraploid rootstocks promote adaptation to different abiotic stresses and promote resilience. Triploid and tetraploid hybrids can be obtained through sexual hybridizations using tetraploid parents (2x × 4x, 4x × 2x, or 4x × 4x), but more knowledge is needed about the inheritance pattern of tetraploid parents to optimize the efficiency of triploid varieties and tetraploid rootstock breeding strategies. In this work, we have analyzed the inheritance pattern of three tetraploid genotypes: ‘Chandler’ pummelo (Citrus maxima) and ‘Cleopatra’ mandarin (Citrus reticulata), which represent two clear examples of autotetraploid plants constituted by the genome of a single species, and the ‘Sevillano’ sour orange, which is an allotetraploid interspecific hybrid between C. maxima and C. reticulata. Polymorphic simple sequence repeat (SSR) and single-nucleotide polymorphism (SNP) markers were used to estimate parental heterozygosity restitution, and allele frequencies for centromeric loci were used to calculate the preferential pairing rate related to the proportion of disomic and tetrasomic segregation. The tetraploid pummelo and mandarin displayed tetrasomic segregation. Sour orange evidenced a clear intermediate inheritance for five of the nine chromosomes (1, 2, 5, 7, and 8), a slight tendency toward tetrasomic inheritance on chromosome 3, and intermediate inheritance with a tendency toward disomy for chromosomes 4, 6, and 9. These results indicate that the interspecific versus intraspecific phylogenomic origin affects preferential pairing and, therefore, the inheritance patterns. Despite its high level of heterozygosity, the important preferential chromosome pairing observed in sour orange results in a limited diversity of the genotypic variability of its diploid gametes, and consequently, a large part of the genetic value of the original diploid sour orange is transferred to the tetraploid progenies. 2024-01-18T08:41:56Z 2024-01-18T08:41:56Z 2023 article publishedVersion Aleza, P., Garavello, M., Rouiss, H., Benedict, A. C., Garcia-Lor, A., Hernandez, M. ... & Ollitrault, P. (2023). Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure. Frontiers in Plant Science, 14, 1327872. 1664-462X https://hdl.handle.net/20.500.11939/8773 10.3389/fpls.2023.1327872 https://www.frontiersin.org/articles/10.3389/fpls.2023.1327872/full en This research was supported by project PID2021-128115OR-I00, funded by the Ministry of Science and Innovation, MCIN/AEI/10.13039/ 501100011033/FEDER, UE and for the project IVIA-GVA 52201 AgroAlimVal. from Instituto Valenciano de Investigaciones Agrarias (this project is subject to being co-financed by the European Union through the ERDF Program 2021-2027 Comunitat Valenciana). info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52201/ES/Relanzando la agroalimentación valenciana para una producción y consumo sostenibles y seguros/AgroAlimVal info:eu-repo/grantAgreement/AEI/Programa Estatal de Generación de Conocimiento y Fortalecimiento Científico y Tecnológico del Sistema de I+D+i/PID2021-128115OR-I00 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Frontiers Media electronico |
| spellingShingle | Triploid Tetrasomic Disomic SSR and SNP markers F30 Plant genetics and breeding F60 Plant physiology and biochemistry F63 Plant physiology - Reproduction Q01 Food science and technology Citrus Seedless varieties Rootstocks Breeding Aleza, Pablo Garavello, Miguel Rouiss, Houssem Benedict, Ana Cristina García-Lor, Andrés Hernández, María Navarro, Luis Ollitrault, Patrick Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure |
| title | Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure |
| title_full | Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure |
| title_fullStr | Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure |
| title_full_unstemmed | Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure |
| title_short | Inheritance pattern of tetraploids pummelo, mandarin, and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure |
| title_sort | inheritance pattern of tetraploids pummelo mandarin and their interspecific hybrid sour orange is highly influenced by their phylogenomic structure |
| topic | Triploid Tetrasomic Disomic SSR and SNP markers F30 Plant genetics and breeding F60 Plant physiology and biochemistry F63 Plant physiology - Reproduction Q01 Food science and technology Citrus Seedless varieties Rootstocks Breeding |
| url | https://hdl.handle.net/20.500.11939/8773 https://www.frontiersin.org/articles/10.3389/fpls.2023.1327872/full |
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