Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness
The influence of light and darkness incubation on ethylene forming activity from 1-aminocyclopropane-1-carboxylic acid (ACC) in citrus (Citrus sinensis L. Osbeck cv. 'Salustiana) mature leaf discs was studied. Leaf discs incubated 48 hours inlight produced 20 times greater ethylene than in darkness....
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| Formato: | Objeto de conferencia |
| Lenguaje: | Inglés |
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International Society of Citriculture
2024
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| Acceso en línea: | https://hdl.handle.net/20.500.11939/8769 |
| _version_ | 1855492555908579328 |
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| author | Zacarias, Lorenzo Tudela, D. Primo-Millo, Eduardo |
| author2 | Goren, R. |
| author_browse | Goren, R. Primo-Millo, Eduardo Tudela, D. Zacarias, Lorenzo |
| author_facet | Goren, R. Zacarias, Lorenzo Tudela, D. Primo-Millo, Eduardo |
| author_sort | Zacarias, Lorenzo |
| collection | ReDivia |
| description | The influence of light and darkness incubation on ethylene forming activity from 1-aminocyclopropane-1-carboxylic acid (ACC) in citrus (Citrus sinensis L. Osbeck cv. 'Salustiana) mature leaf discs was studied. Leaf discs incubated 48 hours inlight produced 20 times greater ethylene than in darkness. Twenty-four hours light and darkness alternative incubations were carried out. In any case, transference of discs from the light to the dark resulted in inhibition of ethylene forming activity. Effects of DCMU (3-(3,4-
dichlorophenyl)-1,1-dimethylurea, inhibitor of photosynthetic electron flow) and KCN (inhibitor of cytochrome oxidase) were studied,, DCMU at 0.1 mM concentration inhibited ethylene forming activity after 48 h incubation in light at 95%.However, ethylene forming activity was not affected by DCMU in the dark. On the other hand, 1mMKCN stimulated considerably ethylene forming activity both in the light and dark. Incubation in a CO, enriched atmosphere did not affect ethylene forming activity in light. Therefore, respiratory CO, release could not be the responsible of ethylene forming activity inhibition in the dark. Increase on ethylene production in light from ACC in mature leaf discs is related with the ethylene forming enzyme (EFE) because of CO, + ion
(inhibitor of EFE activity) reduced highly ethylene production from ACC both in the light and dark. Likewise mannitol (stimulator of EFE activity and ACC synthesis) enhanced ethylene production from ACC both in the light and in the dark. Cycloheximide (inhibitor of protein synthesis) also inhibited ethylene production from ACC. Therefore, enzyme synthesis could be required for the ethylene forming activity from ACC. |
| format | Objeto de conferencia |
| id | ReDivia8769 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | International Society of Citriculture |
| publisherStr | International Society of Citriculture |
| record_format | dspace |
| spelling | ReDivia87692025-04-25T14:51:00Z Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness Zacarias, Lorenzo Tudela, D. Primo-Millo, Eduardo Goren, R. Citrus ethylene F60 Plant physiology and biochemistry CO2 The influence of light and darkness incubation on ethylene forming activity from 1-aminocyclopropane-1-carboxylic acid (ACC) in citrus (Citrus sinensis L. Osbeck cv. 'Salustiana) mature leaf discs was studied. Leaf discs incubated 48 hours inlight produced 20 times greater ethylene than in darkness. Twenty-four hours light and darkness alternative incubations were carried out. In any case, transference of discs from the light to the dark resulted in inhibition of ethylene forming activity. Effects of DCMU (3-(3,4- dichlorophenyl)-1,1-dimethylurea, inhibitor of photosynthetic electron flow) and KCN (inhibitor of cytochrome oxidase) were studied,, DCMU at 0.1 mM concentration inhibited ethylene forming activity after 48 h incubation in light at 95%.However, ethylene forming activity was not affected by DCMU in the dark. On the other hand, 1mMKCN stimulated considerably ethylene forming activity both in the light and dark. Incubation in a CO, enriched atmosphere did not affect ethylene forming activity in light. Therefore, respiratory CO, release could not be the responsible of ethylene forming activity inhibition in the dark. Increase on ethylene production in light from ACC in mature leaf discs is related with the ethylene forming enzyme (EFE) because of CO, + ion (inhibitor of EFE activity) reduced highly ethylene production from ACC both in the light and dark. Likewise mannitol (stimulator of EFE activity and ACC synthesis) enhanced ethylene production from ACC both in the light and in the dark. Cycloheximide (inhibitor of protein synthesis) also inhibited ethylene production from ACC. Therefore, enzyme synthesis could be required for the ethylene forming activity from ACC. 2024-01-18T08:36:05Z 2024-01-18T08:36:05Z 1988 conferenceObject Zacarias, L., Tudela, D. & Primo-Millo, E. (1988). Ethylene forming activity from ACC in citrus leaf discs: influence of light and darkness. Proceedings of the Sixth International Citrus Congress, 315-323. 3-8236-1136-4 https://hdl.handle.net/20.500.11939/8769 en 1988-03 Sixth International Citrus Congress Tel Aviv, Israel Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess International Society of Citriculture electronico |
| spellingShingle | Citrus ethylene F60 Plant physiology and biochemistry CO2 Zacarias, Lorenzo Tudela, D. Primo-Millo, Eduardo Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness |
| title | Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness |
| title_full | Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness |
| title_fullStr | Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness |
| title_full_unstemmed | Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness |
| title_short | Ethylene Forming Activity from ACC in Citrus Leaf Discs: Influence of Light and Darkness |
| title_sort | ethylene forming activity from acc in citrus leaf discs influence of light and darkness |
| topic | Citrus ethylene F60 Plant physiology and biochemistry CO2 |
| url | https://hdl.handle.net/20.500.11939/8769 |
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