Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci

With the advent of high-throughput sequencing, large sets of insect-infecting RNA viruses producing apparent asymptomatic infections are being discovered. In the Mediterranean fruit fly (medfly) Ceratitis capitata, an agricultural key pest of a wide range of fruits, 13 different RNA viruses have bee...

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Autores principales: Hernández-Pelegrín, Luis, García-Martínez, Ricardo, Llácer, Elena, Nieves-Carretero, Lorena, Llopis-Giménez, Ángel, Català-Oltra, Marta, Dembilio, Óscar, Pérez-Hedo, Mertixell, Urbaneja, Alberto, Ros, Vera, Beitia, Francisco J., Herrero, Salvador
Formato: article
Lenguaje:Inglés
Publicado: Springer 2023
Materias:
Acceso en línea:https://hdl.handle.net/20.500.11939/8738
https://link.springer.com/article/10.1007/s10340-023-01617-5
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author Hernández-Pelegrín, Luis
García-Martínez, Ricardo
Llácer, Elena
Nieves-Carretero, Lorena
Llopis-Giménez, Ángel
Català-Oltra, Marta
Dembilio, Óscar
Pérez-Hedo, Mertixell
Urbaneja, Alberto
Ros, Vera
Beitia, Francisco J.
Herrero, Salvador
author_browse Beitia, Francisco J.
Català-Oltra, Marta
Dembilio, Óscar
García-Martínez, Ricardo
Hernández-Pelegrín, Luis
Herrero, Salvador
Llopis-Giménez, Ángel
Llácer, Elena
Nieves-Carretero, Lorena
Pérez-Hedo, Mertixell
Ros, Vera
Urbaneja, Alberto
author_facet Hernández-Pelegrín, Luis
García-Martínez, Ricardo
Llácer, Elena
Nieves-Carretero, Lorena
Llopis-Giménez, Ángel
Català-Oltra, Marta
Dembilio, Óscar
Pérez-Hedo, Mertixell
Urbaneja, Alberto
Ros, Vera
Beitia, Francisco J.
Herrero, Salvador
author_sort Hernández-Pelegrín, Luis
collection ReDivia
description With the advent of high-throughput sequencing, large sets of insect-infecting RNA viruses producing apparent asymptomatic infections are being discovered. In the Mediterranean fruit fly (medfly) Ceratitis capitata, an agricultural key pest of a wide range of fruits, 13 different RNA viruses have been described so far. Recent analysis demonstrated a wide distribution of these viruses in different medfly strains collected worldwide, but little is known about the interactions between those viruses and the medfly host. Previous studies suggested that a higher abundance of Ceratitis capitata nora virus (CcaNV) correlated with a shorter lifespan in adults. Here, we investigated the effect of CcaNV on a broad range of parameters related to host fitness and its interaction with other trophic levels. CcaNV purified from a naturally infected medfly strain was added to the larval diet. Pupal weight, adult emergence, flying ability, and longevity were monitored after oral infections. Our results revealed detrimental effects associated with a CcaNV infection in the medfly, in terms of reduced pupal weight and reduced adult longevity. Moreover, we tested the influence of a CcaNV infection in medflies on the parasitism performance of Aganaspis daci, an endoparasitoid used in biological control programs against medflies. Our results showed that A. daci progeny increased when parasitizing on CcaNV-infected larvae. Overall, we proved that covert RNA viruses can impact the insect ecology, directly affecting its insect host biology and indirectly influencing multitrophic interactions.
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spelling ReDivia87382025-04-25T14:49:25Z Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci Hernández-Pelegrín, Luis García-Martínez, Ricardo Llácer, Elena Nieves-Carretero, Lorena Llopis-Giménez, Ángel Català-Oltra, Marta Dembilio, Óscar Pérez-Hedo, Mertixell Urbaneja, Alberto Ros, Vera Beitia, Francisco J. Herrero, Salvador Covert infections Multitrophic effects Insects ecology RNA viruses Aganaspis daci H10 Pests of plants U30 Research methods Fruit flies Tephritidae Parasitism Ceratitis capitata With the advent of high-throughput sequencing, large sets of insect-infecting RNA viruses producing apparent asymptomatic infections are being discovered. In the Mediterranean fruit fly (medfly) Ceratitis capitata, an agricultural key pest of a wide range of fruits, 13 different RNA viruses have been described so far. Recent analysis demonstrated a wide distribution of these viruses in different medfly strains collected worldwide, but little is known about the interactions between those viruses and the medfly host. Previous studies suggested that a higher abundance of Ceratitis capitata nora virus (CcaNV) correlated with a shorter lifespan in adults. Here, we investigated the effect of CcaNV on a broad range of parameters related to host fitness and its interaction with other trophic levels. CcaNV purified from a naturally infected medfly strain was added to the larval diet. Pupal weight, adult emergence, flying ability, and longevity were monitored after oral infections. Our results revealed detrimental effects associated with a CcaNV infection in the medfly, in terms of reduced pupal weight and reduced adult longevity. Moreover, we tested the influence of a CcaNV infection in medflies on the parasitism performance of Aganaspis daci, an endoparasitoid used in biological control programs against medflies. Our results showed that A. daci progeny increased when parasitizing on CcaNV-infected larvae. Overall, we proved that covert RNA viruses can impact the insect ecology, directly affecting its insect host biology and indirectly influencing multitrophic interactions. 2023-11-15T07:55:03Z 2023-11-15T07:55:03Z 2023 article publishedVersion Hernández-Pelegrín, L., García-Martínez, R., Llácer, E., Nieves, L., Llopis-Giménez, Á., Catalá-Oltra, M., ... & Herrero, S. (2024). Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci. Journal of Pest Science, 97(1), 269-280. 1612-4766 (Electronic ISSN) 1612-4758 (Print ISSN) https://hdl.handle.net/20.500.11939/8738 10.1007/s10340-023-01617-5 https://link.springer.com/article/10.1007/s10340-023-01617-5 en Open Access funding provided thanks to the CRUE-CSIC agreement with Springer Nature. This study was supported by the INSECT DOCTORS program, funded under the European Union Horizon 2020 Framework Program for Research and Innovation (Marie Sklodowska-Curie Grant Agreement 859850) and projects IVIA-52202 and IVIA-51916 from Instituto Valenciano de Investigaciones Agrarias (both projects are susceptible of being co-financed by the European Union through the FEDER). V.I.D.R. is supported by a VIDI-grant of the Dutch Research Council (NWO; VI. Vidi. 192.041). info:eu-repo/grantAgreement/ERDF/PCV 2021-2027/52202/ES/Control biológico de plagas y enfermedades como pieza clave en el impulso de la Sostenibilidad de los cultivos agrarios de la Comunidad Valenciana/SOSTENIBLE info:eu-repo/grantAgreement/EC/H2020/859850 Attribution-NonCommercial-NoDerivatives 4.0 Internacional Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess Springer electronico
spellingShingle Covert infections
Multitrophic effects
Insects ecology
RNA viruses
Aganaspis daci
H10 Pests of plants
U30 Research methods
Fruit flies
Tephritidae
Parasitism
Ceratitis capitata
Hernández-Pelegrín, Luis
García-Martínez, Ricardo
Llácer, Elena
Nieves-Carretero, Lorena
Llopis-Giménez, Ángel
Català-Oltra, Marta
Dembilio, Óscar
Pérez-Hedo, Mertixell
Urbaneja, Alberto
Ros, Vera
Beitia, Francisco J.
Herrero, Salvador
Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci
title Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci
title_full Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci
title_fullStr Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci
title_full_unstemmed Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci
title_short Covert infection with an RNA virus affects medfly fitness and the interaction with its natural parasitoid Aganaspis daci
title_sort covert infection with an rna virus affects medfly fitness and the interaction with its natural parasitoid aganaspis daci
topic Covert infections
Multitrophic effects
Insects ecology
RNA viruses
Aganaspis daci
H10 Pests of plants
U30 Research methods
Fruit flies
Tephritidae
Parasitism
Ceratitis capitata
url https://hdl.handle.net/20.500.11939/8738
https://link.springer.com/article/10.1007/s10340-023-01617-5
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