Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus
The excessive accumulation of chloride (Cl−) in leaves due to salinity is frequently related to decreased yield in citrus. Two salt tolerance experiments to detect quantitative trait loci (QTLs) for leaf concentrations of Cl−, Na+, and other traits using the same reference progeny derived from the s...
| Main Authors: | , , , , , , , , , , |
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| Format: | Artículo |
| Language: | Inglés |
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MDPI
2023
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| Online Access: | https://hdl.handle.net/20.500.11939/8737 https://www.mdpi.com/1422-0067/24/21/15759 https://www.ebi.ac.uk/ena/browser/view/PRJEB61142 https://www.ncbi.nlm.nih.gov/bioproject/1070151 |
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| author | Asins, María J. Bullones, Amanda Raga, Verónica Romero-Aranda, María R. Espinosa, Jesús Triviñi, Juan C. Bernet, Guillermo P. Traverso, Jose A. Carbonell, Emilio A. Claros-Díaz, Manuel Gonzalo Belver, Andres |
| author_browse | Asins, María J. Belver, Andres Bernet, Guillermo P. Bullones, Amanda Carbonell, Emilio A. Claros-Díaz, Manuel Gonzalo Espinosa, Jesús Raga, Verónica Romero-Aranda, María R. Traverso, Jose A. Triviñi, Juan C. |
| author_facet | Asins, María J. Bullones, Amanda Raga, Verónica Romero-Aranda, María R. Espinosa, Jesús Triviñi, Juan C. Bernet, Guillermo P. Traverso, Jose A. Carbonell, Emilio A. Claros-Díaz, Manuel Gonzalo Belver, Andres |
| author_sort | Asins, María J. |
| collection | ReDivia |
| description | The excessive accumulation of chloride (Cl−) in leaves due to salinity is frequently related to decreased yield in citrus. Two salt tolerance experiments to detect quantitative trait loci (QTLs) for leaf concentrations of Cl−, Na+, and other traits using the same reference progeny derived from the salt-tolerant Cleopatra mandarin (Citrus reshni) and the disease-resistant donor Poncirus trifoliata were performed with the aim to identify repeatable QTLs that regulate leaf Cl− (and/or Na+) exclusion across independent experiments in citrus, as well as potential candidate genes involved. A repeatable QTL controlling leaf Cl− was detected in chromosome 6 (LCl-6), where 23 potential candidate genes coding for transporters were identified using the C. clementina genome as reference. Transcriptomic analysis revealed two important candidate genes coding for a member of the nitrate transporter 1/peptide transporter family (NPF5.9) and a major facilitator superfamily (MFS) protein. Cell wall biosynthesis- and secondary metabolism-related processes appeared to play a significant role in differential gene expression in LCl-6. Six likely gene candidates were mapped in LCl-6, showing conserved synteny in C. reshni. In conclusion, markers to select beneficial Cleopatra mandarin alleles of likely candidate genes in LCl-6 to improve salt tolerance in citrus rootstock breeding programs are provided. |
| format | Artículo |
| id | ReDivia8737 |
| institution | Instituto Valenciano de Investigaciones Agrarias (IVIA) |
| language | Inglés |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | MDPI |
| publisherStr | MDPI |
| record_format | dspace |
| spelling | ReDivia87372025-04-25T14:49:25Z Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus Asins, María J. Bullones, Amanda Raga, Verónica Romero-Aranda, María R. Espinosa, Jesús Triviñi, Juan C. Bernet, Guillermo P. Traverso, Jose A. Carbonell, Emilio A. Claros-Díaz, Manuel Gonzalo Belver, Andres QTL analysis Citrus reshni Rootstock breeding Root growth Cl-Homeostasis F30 Plant genetics and breeding U30 Research methods F62 Plant physiology - Growth and development P33 Soil chemistry and physics QTL (quantitative trait loci) Poncirus trifoliata Yields Plasticity The excessive accumulation of chloride (Cl−) in leaves due to salinity is frequently related to decreased yield in citrus. Two salt tolerance experiments to detect quantitative trait loci (QTLs) for leaf concentrations of Cl−, Na+, and other traits using the same reference progeny derived from the salt-tolerant Cleopatra mandarin (Citrus reshni) and the disease-resistant donor Poncirus trifoliata were performed with the aim to identify repeatable QTLs that regulate leaf Cl− (and/or Na+) exclusion across independent experiments in citrus, as well as potential candidate genes involved. A repeatable QTL controlling leaf Cl− was detected in chromosome 6 (LCl-6), where 23 potential candidate genes coding for transporters were identified using the C. clementina genome as reference. Transcriptomic analysis revealed two important candidate genes coding for a member of the nitrate transporter 1/peptide transporter family (NPF5.9) and a major facilitator superfamily (MFS) protein. Cell wall biosynthesis- and secondary metabolism-related processes appeared to play a significant role in differential gene expression in LCl-6. Six likely gene candidates were mapped in LCl-6, showing conserved synteny in C. reshni. In conclusion, markers to select beneficial Cleopatra mandarin alleles of likely candidate genes in LCl-6 to improve salt tolerance in citrus rootstock breeding programs are provided. 2023-11-15T07:52:23Z 2023-11-15T07:52:23Z 2023 article publishedVersion Asins, M. J., Bullones, A., Raga, V., Romero-Aranda, M. R., Espinosa, J., Triviño, J. C. et al. (2023). Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus. International Journal of Molecular Sciences, 24(21), 15759. 1422-0067 https://hdl.handle.net/20.500.11939/8737 10.3390/ijms242115759 https://www.mdpi.com/1422-0067/24/21/15759 https://www.ebi.ac.uk/ena/browser/view/PRJEB61142 https://www.ncbi.nlm.nih.gov/bioproject/1070151 en The study was co-funded by grants RTA2011-00132-C2 (M.J.A.), AGL2014-56675-R (M.J.A.), AGL2017-82452-C2 (A.Be., M.J.A.), and PID2021-124599OB-I00 (A.Be.) from the Spanish Ministry of Science and Innovation/the Spanish Research Agency, by grant 51917 (M.J.A.) from the Generalitat Valenciana, as well as the European Regional Development Fund (ERDF), MCIN/AEI/10.13039/501100011033, and ERDF “A way of making Europe”. info:eu-repo/grantAgreement/MICINN/Programa Nacional de Investigación Fundamental/RTA2011-00132-C02-02/ES/Pre-Mejora genética en limonero info:eu-repo/grantAgreement/MINECO/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2014-56675-R/ES/Aprovechamiento de recursos fitogenéticos para la diversificación de patrones eficientes en la adquisición de hierro info:eu-repo/grantAgreement/AEI/Programa Estatal de I+D+I Orientada a los Retos de la Sociedad/AGL2017- 82452-C2/ES/Transportadores de iones probablemente implicados en QTLS de gran efecto sobre la tolerancia a salinidad en términos de producción en cítricos y tomate info:eu-repo/grantAgreement/AEI/Proyectos de Generación de Conocimiento/PID2021-124599OB-100 Attribution-NonCommercial-NoDerivatives 4.0 Internacional http://creativecommons.org/licenses/by-nc-nd/4.0/ openAccess MDPI electronico |
| spellingShingle | QTL analysis Citrus reshni Rootstock breeding Root growth Cl-Homeostasis F30 Plant genetics and breeding U30 Research methods F62 Plant physiology - Growth and development P33 Soil chemistry and physics QTL (quantitative trait loci) Poncirus trifoliata Yields Plasticity Asins, María J. Bullones, Amanda Raga, Verónica Romero-Aranda, María R. Espinosa, Jesús Triviñi, Juan C. Bernet, Guillermo P. Traverso, Jose A. Carbonell, Emilio A. Claros-Díaz, Manuel Gonzalo Belver, Andres Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus |
| title | Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus |
| title_full | Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus |
| title_fullStr | Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus |
| title_full_unstemmed | Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus |
| title_short | Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus |
| title_sort | combining genetic and transcriptomic approaches to identify transporter coding genes as likely responsible for a repeatable salt tolerance qtl in citrus |
| topic | QTL analysis Citrus reshni Rootstock breeding Root growth Cl-Homeostasis F30 Plant genetics and breeding U30 Research methods F62 Plant physiology - Growth and development P33 Soil chemistry and physics QTL (quantitative trait loci) Poncirus trifoliata Yields Plasticity |
| url | https://hdl.handle.net/20.500.11939/8737 https://www.mdpi.com/1422-0067/24/21/15759 https://www.ebi.ac.uk/ena/browser/view/PRJEB61142 https://www.ncbi.nlm.nih.gov/bioproject/1070151 |
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